Inhibitory action of novel aromatic diamine compound on lipopolysaccharide‐induced nuclear translocation of NF‐κB without affecting IκB degradation
Abstract
4‐Methyl‐N 1‐(3‐phenyl‐propyl)‐benzene‐1,2‐diamine (JSH‐23) is a novel chemically synthetic compound. The aromatic diamine JSH‐23 compound exhibited inhibitory effect with an IC 50 value of 7.1 μM on nuclear factor (NF)‐κB transcriptional activity in lipopolysaccharide (LPS)‐stimulated macrophages RAW 264.7, and interfered LPS‐induced nuclear translocation of NF‐κB without affecting IκB degradation. This mechanism of action is very rare for controlling NF‐κB activation. Furthermore, the compound inhibited not only LPS‐induced expressions of tumor necrosis factor‐α, interleukin (IL)‐1β, IL‐6 and inducible nitric oxide synthase and cyclooxygenase‐2 but also LPS‐induced apoptosis of the RAW 264.7 cells.
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JSH-23 suppresses NF-κB signaling and SB-431542 suppresses TGF-β1/Smad3 signaling.
“JSH-23 and SB-431542 can suppress NF-κB and TGF-β1/Smad3 signaling pathways respectively and thus might inhibit platelet aggregation in endometriosis91,92.”
From Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells (2020) · cites this paper as [91]
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- Authors
- Shin, H.-M., Kim, M.-H., Kim, B. H., Jung, S.-H., Kim, Y. S., Park, H. J., Hong, J. T., Min, K. R., & Kim, Y.
- Affiliations
- Chungbuk National University (KR)
- Chungnam National University (KR)
- Seoul National University (KR)
- Corresponding author
- Kim, Youngsoo
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-07
- Volume
- 571
- Issue
- 1-3
- Pages
- 50-54
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2086559549
- Cited by
- 269
- References
- 24
- Citations by year
- 2026: 19 · 2025: 20 · 2024: 18 · 2023: 14 · 2022: 12 · 2021: 25 · 2020: 15 · 2019: 10 · 2018: 18 · 2017: 14 · 2016: 9 · 2015: 18 · 2014: 11 · 2013: 7 · 2012: 8
- FWCI
- 2.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korea Science and Engineering Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- JSH-23, aromatic diamines, NF-κB nuclear translocation, lipopolysaccharide, macrophages, RAW 264.7 macrophages, IκB degradation, NF-κB transcriptional activity, TNF-α, IL-1β, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, apoptosis, anti-inflammatory agents
- MeSH terms
- NF-KappaB Inhibitor alpha, Animals, Cell Line, Cell Nucleus, Cell Survival, Escherichia coli, Gene Expression Regulation, Inflammation, Kinetics, Lipopolysaccharides, Macrophages, Cytokines, NF-kappa B, Nitric Oxide Synthase, Reverse Transcriptase Polymerase Chain Reaction, I-kappa B Proteins, Protein Transport, Mice, Nitric Oxide Synthase Type II
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