Structural basis of eukaryotic gene transcription
Abstract
An RNA polymerase II promoter has been isolated in transcriptionally activated and repressed states. Topological and nuclease digestion analyses have revealed a dynamic equilibrium between nucleosome removal and reassembly upon transcriptional activation, and have further shown that nucleosomes are removed by eviction of histone octamers rather than by sliding. The promoter, once exposed, assembles with RNA polymerase II, general transcription factors, and Mediator in a ∼3 MDa transcription initiation complex. X‐ray crystallography has revealed the structure of RNA polymerase II, in the act of transcription, at atomic resolution. Extension of this analysis has shown how nucleotides undergo selection, polymerization, and eventual release from the transcribing complex. X‐ray and electron crystallography have led to a picture of the entire transcription initiation complex, elucidating the mechanisms of promoter recognition, DNA unwinding, abortive initiation, and promoter escape.
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CHO cells are refractory to infection by human viruses, which minimizes biosafety risks for commercial production.
“Furthermore, these cells are refractory to infection by human viruses, which minimizes biosafety risks for commercial production purpose (Boeger et al., 2005).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Boeger et al., 2005
Cites
Structural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
Cramer, P., Bushnell, D. A., & Kornberg, R. D. (2001) · Science · doi:10.1126/science.1059493 · Cited by 1,247 · see citing works
Self-citationA novel mediator between activator proteins and the RNA polymerase II transcription apparatus
Kelleher, R. J., Flanagan, P. M., & Kornberg, R. D. (1990) · Cell · doi:10.1016/0092-8674(90)90685-8 · Cited by 450 · see citing works
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In vivo dynamics of RNA polymerase II transcription
Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., & Singer, R. H. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1280 · Cited by 665 · see citing works
Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Boeger, H., Bushnell, D. A., Davis, R., Griesenbeck, J., Lorch, Y., Strattan, J. S., Westover, K. D., & Kornberg, R. D.
- Affiliations
- Stanford Medicine (US)
- Corresponding author
- Kornberg, Roger D.
- ORCID
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-11-23
- Volume
- 579
- Issue
- 4
- Pages
- 899-903
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2043457799
- Cited by
- 151
- References
- 2
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2022: 7 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 9 · 2012: 6
- FWCI
- 6.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- RNA polymerase II, promoter structure, transcription initiation complex, histone eviction, Mediator complex, general transcription factors, transcriptional activation, transcriptional repression, nucleotide selection, DNA unwinding, abortive initiation, promoter escape, promoter identification
- MeSH terms
- Eukaryotic Cells, Nucleosomes, Promoter Regions, Genetic, RNA Polymerase II, Transcription, Genetic, Molecular Structure, Crystallography, X-Ray
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