The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Highly efficient protein trans‐splicing by a naturally split DnaE intein from Nostoc punctiforme
Open accessAbstract
Protein trans‐splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non‐native exteins in Escherichia coli. Npu DnaE possesses robust trans‐splicing activity with an efficiency of >98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 (Ssp DnaE). Both the N‐ and C‐terminal parts of the split Npu DnaE intein can be substituted with the corresponding fragment of Ssp DnaE without loss of trans‐splicing activity. Protein splicing with the Npu DnaE N is also more tolerant of amino acid substitutions in the C‐terminal extein sequence.
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Cites
Production of cyclic peptides and proteins in vivo
Scott, C. P., Abel-Santos, E., Wall, M., Wahnon, D. C., & Benkovic, S. J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13638 · Cited by 402
Cited by
Inteins: nature's gift to protein chemists
Shah, N. H., & Muir, T. W. (2014) · Chem. Sci. · doi:10.1039/c3sc52951g · Cited by 404
Continuous evolution of base editors with expanded target compatibility and improved activity
Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt, J. R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0193-0 · Cited by 347
Tornabene, P., Trapani, I., Minopoli, R., Centrulo, M., Lupo, M., de Simone, S., Tiberi, P., Dell’Aquila, F., Marrocco, E., Iodice, C., et al. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav4523 · Cited by 192
Nature's recipe for splitting inteins
Aranko, A. S., Wlodawer, A., & Iwai, H. (2014) · Protein Engineering Design and Selection · doi:10.1093/protein/gzu028 · Cited by 116
Self-citationExtein Residues Play an Intimate Role in the Rate-Limiting Step of Protein Trans -Splicing
Shah, N. H., Eryilmaz, E., Cowburn, D., & Muir, T. W. (2013) · Journal of the American Chemical Society · doi:10.1021/ja401015p · Cited by 84
Metadata
- Authors
- Iwai, H., Züger, S., Jin, J., & Tam, P.-H.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2006-02-24
- Volume
- 580
- Issue
- 7
- Pages
- 1853-1858
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2170501485
- Cited by
- 273
- References
- 1
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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