The cartilage specific microRNA‐140 targets histone deacetylase 4 in mouse cells
Abstract
MicroRNAs (miRNA) are short RNA molecules regulating the expression of specific mRNAs. We investigated the expression pattern and potential targets of mouse miR‐140 and found that miR‐140 is specifically expressed in cartilage tissues of mouse embryos during both long and flat bone development. MiR‐140 expression was detected in the limbs of E11.5 embryos in the primorida of future bones both in the fore and hindlimb and across autopod, zeugopod and stylopod. All digits of E14.5 fore‐ and hindlimbs showed accumulation of miR‐140, except the first digit of the hindlimb. MiR‐140 expression was also detected in the cartilagenous base of E17.5 skulls and in the sternum, the proximal rib heads and the developing vertebral column of E15.5 embryos. A potential target of miR‐140, histone deacetylase 4, was validated experimentally and the possible role of miR‐140 in long bone development is discussed.
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miR-140-5p and miR-140-3p are abundantly and relatively specifically expressed in chondrocytes.
“miR-140-5p and miR-140-3p (collectively miR-140) are abundantly and relatively specifically expressed in chondrocytes14,15”
From Gain-of-function mutation of microRNA-140 in human skeletal dysplasia (2019) · cites this paper as [15]
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“…evolutionarily conserved cell type-specific function 11–13 . miR-140-5p and miR-140-3p (collectively miR-140) are abundantly and relatively specifically expressed in chondrocytes 14,15 , and loss of miR-140, encoded by Mir140, in mice leads to short stature and craniofacial abnormalities 6,16 .”
Epigenetic genes and epilepsy — emerging mechanisms and clinical applications
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- Authors
- Tuddenham, L., Wheeler, G., Ntounia-Fousara, S., Waters, J., Hajihosseini, M. K., Clark, I., & Dalmay, T.
- Affiliations
- University of East Anglia (GB)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2006-07-05
- Volume
- 580
- Issue
- 17
- Pages
- 4214-4217
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2014028055
- Cited by
- 422
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 12 · 2023: 16 · 2022: 13 · 2021: 15 · 2020: 23 · 2019: 17 · 2018: 12 · 2017: 15 · 2016: 31 · 2015: 40 · 2014: 37 · 2013: 31 · 2012: 44
- FWCI
- 10.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Funders
- Wellcome Trust (068167)
- Topics
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- miR-140, microRNA, HDAC4, cartilage, long bone development, limb development, embryonic development, gene expression patterns, miRNA target validation, mouse embryo
- MeSH terms
- Animals, Cartilage, Hindlimb, Histone Deacetylases, Osteogenesis, Gene Expression Regulation, Enzymologic, In Situ Hybridization, Gene Expression Regulation, Developmental, Skull Base, MicroRNAs, Mice
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