MiR‐483–5p suppresses the proliferation of glioma cells via directly targeting ERK1

Abstract

MicroRNAs (miRNAs) exhibit tumor‐specific expression signatures and play crucial roles in tumorigenesis by targeting oncogenes. Here, through analyzing the miRNA‐array profiles of human glioblastoma tissues and the adjacent normal brain tissues, we found miR‐483–5p was significantly down‐regulated in gliomas, which was confirmed in both human glioma specimens and cell lines. The overexpression of miR‐483–5p suppressed glioma cell proliferation and induced a G0/G1 arrest. In contrast, miR‐483–5p inhibition promoted cell proliferation. Furthermore, by a dual‐luciferase reporter assay and expression analysis, we identified extracellular signal‐regulated kinase 1 (ERK1) as a direct target of miR‐483–5p. ERK1 knockdown can block cell proliferation induced by miR‐483–5p inhibition. Thus, our findings provide the first evidence that miR‐483–5p can serve as a tumor suppressor in gliomas.