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Cited by 222Viral Infectious Diseases and Gene Expression in InsectsProtein purification and stabilityAnimal Genetics and Reproduction

Recent advances in mammalian protein production

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Bandaranayake, Ashok D., Almo, Steven C.·2014·FEBS Letters
DOI 10.1016/j.febslet.2013.11.035PMC PMC3924552
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Abstract

Mammalian protein production platforms have had a profound impact in many areas of basic and applied research, and an increasing number of blockbuster drugs are recombinant mammalian proteins. With global sales of these drugs exceeding US$120 billion per year, both industry and academic research groups continue to develop cost effective methods for producing mammalian proteins to support pre‐clinical and clinical evaluations of potential therapeutics. While a wide range of platforms have been successfully exploited for laboratory use, the bulk of recent biologics have been produced in mammalian cell lines due to the requirement for post translational modification and the biosynthetic complexity of the target proteins. In this review we highlight the range of mammalian expression platforms available for recombinant protein production, as well as advances in technologies for the rapid and efficient selection of highly productive clones.

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  • Successful apoptosis inhibition was also achieved by overexpression of other related anti-apoptotic proteins.

    “Successful apoptosis inhibition was also achieved by overexpression of other related anti-apoptotic proteins (Durocher and Butler, 2009;Bandaranayake and Almo, 2014).”

    From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Bandaranayake and Almo, 2014

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Authors
Bandaranayake, A. D., & Almo, S. C.
Affiliations
Albert Einstein College of Medicine (US)
Corresponding author
Bandaranayake, Ashok D.
ORCID
Almo, Steven C.
Venue
FEBS Letters
Publisher
Wiley
Published
2013-12-06
Volume
588
Issue
2
Pages
253-260
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
DOI
10.1016/j.febslet.2013.11.035
PMC
PMC3924552
OpenAlex
W2052407981
Cited by
222
References
2
Citations by year
2026: 10 · 2025: 8 · 2024: 12 · 2023: 14 · 2022: 10 · 2021: 18 · 2020: 28 · 2019: 33 · 2018: 25 · 2017: 18 · 2016: 21 · 2015: 18 · 2014: 6
FWCI
1.41
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institutes of Health (NIH) (GM094662, GM094665, CA013330)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords
recombinant protein expression, mammalian cell expression systems, mammalian cell lines, post-translational modification, biologics, clone selection
MeSH terms
Animals, Cell Line, Genetic Engineering, Humans, Mammals, Recombinant Proteins, Gene Transfer Techniques

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