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Cited by 189Sperm and Testicular FunctionReproductive Biology and FertilityReproductive biology and impacts on aquatic species

Impact of sperm morphology on DNA damage caused by oxidative stress induced by β-nicotinamide adenine dinucleotide phosphate

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Said, Tamer M., Agarwal, Ashok, Sharma, Rakesh K., Thomas, Anthony J., Sikka, Suresh C.·2005·Fertility and Sterility
DOI 10.1016/j.fertnstert.2004.06.056
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Abstract

Objective To investigate the role of DNA damage induced by beta-nicotinamide adenine dinucleotide phosphate (NADPH) in human spermatozoa. Design Prospective controlled study. Setting Male infertility clinic at the Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio. Patient(s) Twenty-eight men undergoing infertility screening. Intervention(s) Chemiluminescence assay and terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay coupled flow cytometry after incubating mature and immature sperm separated by density gradient with 5 mM NADPH for 0, 3, and 24 hours. Main outcome measure(s) Reactive oxygen species (ROS) generation (10(6) counted photons per minute/10(6) sperm) and percentage of spermatozoa with fragmented DNA. Result(s) Immature sperm from teratozoospermic semen samples were characterized by a statistically significant presence of cytoplasmic residues in the mid-piece when compared with mature normozoospermic samples. Increased ROS production was observed in spermatozoa rich in cytoplasmic residues that showed a statistically significant positive correlation with sperm DNA damage in a time-dependent manner. Conclusion(s) Immature sperm contain high nicotinamide adenine dinucleotide phosphate (NADPH) in cytoplasmic droplets, but it has not yet been clear whether abnormal sperm morphology plays any role in sperm DNA damage induced by oxidative stress. Our data support the role of NAPDH in ROS-mediated sperm DNA damage and suggest that abnormal sperm morphology combined with elevated ROS production may serve as a useful indicator of potential damage to sperm DNA.

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  • Causes for increased oxidative stress in teratozoospermic men include the increase of MDA, lipid peroxidation, iron, and NADPH, which is positively correlated with damage to sperm DNA.

    “Causes for increased oxidative stress in teratozoospermic men include the increase of MDA (malondialdehyde), lipid peroxidation, iron, and NADPH (nicotinamide adenine dinucleotide phosphate), which is positively correlated with the damage to sperm DNA (74,75).”

    From Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review (2024) · cites this paper as [75]

    The Discussion (page unavailable) says that cytoplasmic residues in immature sperm generate NADPH, which stimulates ROS production, and links these sperm to oxidative DNA damage in teratozoospermic samples. The cited passages do not establish increased MDA, lipid peroxidation, or iron as causes of oxidative stress in these men, or correlate those factors with sperm DNA damage. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Said, T. M., Agarwal, A., Sharma, R. K., Thomas, A. J., & Sikka, S. C.
Affiliations
  • Cleveland Clinic (US)
  • Tulane University (US)
Corresponding author
Agarwal, Ashok
ORCID
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2005-01
Volume
83
Issue
1
Pages
95-103
ISSN
0015-0282 (ISSN-L)
DOI
10.1016/j.fertnstert.2004.06.056
OpenAlex
W2125390422
Cited by
189
References
0
Citations by year
2026: 5 · 2025: 7 · 2024: 7 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 15 · 2019: 5 · 2018: 3 · 2017: 9 · 2016: 6 · 2015: 11 · 2014: 11 · 2013: 14 · 2012: 19
FWCI
8.69
Type
journal article
Language
en
Open access
Licence
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Keywords
sperm morphology, sperm DNA damage, NADPH, oxidative stress, reactive oxygen species, male infertility, teratozoospermia, sperm DNA fragmentation, chemiluminescence, TUNEL
MeSH terms
DNA Damage, Humans, Male, NADP, Prospective Studies, Spermatozoa, Oxidative Stress

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