Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites
Open accessAbstract
Objective To investigate whether gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) are expressed at tubal ectopic pregnancy sites, and to study the potential role of GnRH signaling in regulating immortalized human trophoblast cell viability.Design Immunohistochemical and experimental studies.Setting Academic research laboratory.Patient(s) Fallopian tube implantation sites (n = 25) were collected from women with ectopic pregnancy. First-trimester human placenta biopsies (n = 5) were obtained from elective terminations of pregnancy.Intervention(s) None.Main outcome measure(s) GnRH and GnRHR expression was examined by means of immunohistochemistry and histoscoring. Trophoblastic BeWo choriocarcinoma and immortalized extravillous trophoblast (HTR-8/SVneo) cell viability was examined by means of cell counting after incubation with GnRH and/or GnRH antagonist (Antide).Result(s) GnRH and GnRHR immunoreactivity was detected in cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast in all women with tubal pregnancy. GnRH immunoreactivity was higher and GnRHR immunoreactivity lower in syncytiotrophoblast compared with cytotrophoblast. GnRH and GnRHR immunoreactivity was detected in adjacent fallopian tube epithelium. Whereas neither GnRH nor Antide altered HTR-8/SVneo cell viability, treatment with GnRH significantly increased the overall cell viability of BeWo cells at 48 and 72 hours, and these effects were abolished by pretreatment with Antide.Conclusion(s) GnRH and GnRHR are expressed in trophoblast cell populations and fallopian tube epithelium at tubal ectopic pregnancy sites. GnRH increases BeWo cell viability, an effect mediated by the GnRHR. Further work is required to investigate the potential role of GnRH signaling in ectopic pregnancy.
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GnRHR has been shown to be important in placental development and function; it is even expressed at the implantation sites of ectopic pregnancy.
“GnRHR has been shown to be important in placental development and function; it is even expressed at the implantation sites of ectopic pregnancy63.”
From Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse (2019) · cites this paper as [63]
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Cites
Hypothalamic programming of systemic ageing involving IKK-β, NF-κB and GnRH
Zhang, G., Li, J., Purkayastha, S., Tang, Y., Zhang, H., Yin, Y., Li, B., Liu, G., & Cai, D. (2013) · Nature · doi:10.1038/nature12143 · Cited by 834 · see citing works
Gonadotropin-Releasing Hormone Receptors
Millar, R. P., Lu, Z.-L., Pawson, A. J., Flanagan, C. A., Morgan, K., & Maudsley, S. R. (2004) · Endocrine Reviews · doi:10.1210/er.2003-0002 · Cited by 791 · see citing works
Farquhar, C. M. (2005) · The Lancet · doi:10.1016/s0140-6736(05)67103-6 · Cited by 472 · see citing works
Millar, R. P. (2005) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2005.05.032 · Cited by 423 · see citing works
Diagnosis and management of ectopic pregnancy
Sivalingam, V. N., Duncan, W. C., Kirk, E., et al. (2011) · Journal of Family Planning and Reproductive Health Care · doi:10.1136/jfprhc-2011-0073 · Cited by 328 · see citing works
Molecular Biology of Gonadotropin-Releasing Hormone (GnRH)-I, GnRH-II, and Their Receptors in Humans
Cheng, C. K., & Leung, P. C. K. (2005) · Endocrine Reviews · doi:10.1210/er.2003-0039 · Cited by 298 · see citing works
Self-citationGnRH Receptors in Cancer: From Cell Biology to Novel Targeted Therapeutic Strategies
Limonta, P., Marelli, M. M., Mai, S., Motta, M., Martini, L., & Moretti, R. M. (2012) · Endocrine Reviews · doi:10.1210/er.2012-1014 · Cited by 162 · see citing works
Kamwendo, F. (2000) · Sexually Transmitted Infections · doi:10.1136/sti.76.1.28 · Cited by 140 · see citing works
Diagnosis and management of ectopic pregnancy
Jurkovic, D., & Wilkinson, H. (2011) · BMJ · doi:10.1136/bmj.d3397 · Cited by 140 · see citing works
Nenutil, R., Šmardová, J., Pavlová, Š., Hanzelková, Z., Müller, P., Fabián, P., Hrstka, R., Janotova, P., Radina, M., Lane, D. P., et al. (2005) · The Journal of Pathology · doi:10.1002/path.1838 · Cited by 135 · see citing works
Ruan, H.-C., Zhu, X., Luo, Q., et al. (2006) · Human Reproduction · doi:10.1093/humrep/del215 · Cited by 109 · see citing works
Ehsanian, R., Brown, M. S., Lü, H., Yang, X., Pattatheyil, A., Yan, B., Duggal, P., Chuang, R. J., Doondeea, J. B., Feller, S. M., et al. (2010) · Oncogene · doi:10.1038/onc.2010.339 · Cited by 94 · see citing works
Wolfahrt, S. (1998) · Molecular Human Reproduction · doi:10.1093/molehr/4.10.999 · Cited by 69 · see citing works
Chou, C.-S., Beristain, A. G., MacCalman, C. D., et al. (2004) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-031636 · Cited by 57 · see citing works
Liu, J., MacCalman, C. D., Wang, Y., & Leung, P. C. K. (2009) · Molecular Endocrinology · doi:10.1210/me.2008-0451 · Cited by 56 · see citing works
Self-citationPeng, B., Zhu, H., Ma, L., Wang, Y., Klausen, C., & Leung, P. C. K. (2015) · Endocrinology · doi:10.1210/en.2014-1871 · Cited by 56 · see citing works
Self-citationTanrıverdi, F., González-Martínez, D. A., Hu, Y., et al. (2005) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.2005.02904.x · Cited by 55 · see citing works
Gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling in the placenta
Sasaki, K., & Norwitz, E. R. (2011) · Current Opinion in Endocrinology Diabetes and Obesity · doi:10.1097/med.0b013e32834cd3b0 · Cited by 43 · see citing works
Bützow, R. C. (1982) · International Journal of Cancer · doi:10.1002/ijc.2910290103 · Cited by 40 · see citing works
GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/9 expression
Peng, B., Zhu, H., Klausen, C., et al. (2015) · Molecular Human Reproduction · doi:10.1093/molehr/gav070 · Cited by 40 · see citing works
Self-citationPeng, B., Zhu, H., & Leung, P. C. K. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-1897 · Cited by 38 · see citing works
Self-citationThe expression of receptivity markers in the fallopian tube epithelium
Makrigiannakis, A., Karamouti, M., Petsas, G. K., et al. (2009) · Histochemistry and Cell Biology · doi:10.1007/s00418-009-0593-1 · Cited by 35 · see citing works
Casañ, E. M., Raga, F., Krüssel, J.-S., Wen, Y., NEZHAT, C. R., & Polan, M. L. (1998) · Fertility and Sterility · doi:10.1016/s0015-0282(98)00125-3 · Cited by 32 · see citing works
The role of leukemia inhibitory factor in tubal ectopic pregnancy
Krishnan, T., Winship, A., Sonderegger, S., et al. (2013) · Placenta · doi:10.1016/j.placenta.2013.09.003 · Cited by 30 · see citing works
Mechanisms of disease: the endocrinology of ectopic pregnancy
Horne, A. W., & Critchley, H. (2012) · Expert Reviews in Molecular Medicine · doi:10.1017/erm.2011.2 · Cited by 28 · see citing works
Self-citationBarneal, E. R., Kaplan, M., & Naor, Z. (1991) · Human Reproduction · doi:10.1093/oxfordjournals.humrep.a137485 · Cited by 26 · see citing works
Currie, W. D., Steele, G. L., Yuen, B. H., et al. (1992) · Endocrinology · doi:10.1210/endo.130.5.1572299 · Cited by 26 · see citing works
Role of GnRH–GnRH receptor signaling at the maternal-fetal interface
Lee, H. J., Snegovskikh, V. V., Park, J. S., Foyouzi, N., Han, K. T., Hodgson, E. J., Guller, S., & Norwitz, E. R. (2010) · Fertility and Sterility · doi:10.1016/j.fertnstert.2010.03.016 · Cited by 21 · see citing works
Gonadotropin-Releasing Hormone Stimulates Biliary Proliferation by Paracrine/Autocrine Mechanisms
Ray, D., Han, Y., Franchitto, A., DeMorrow, S., Meng, F., Venter, J. K., McMillin, M., Kennedy, L. L., Francis, H., Onori, P., et al. (2015) · American Journal Of Pathology · doi:10.1016/j.ajpath.2014.12.004 · Cited by 20 · see citing works
Leukemia inhibitory factor (LIF) is immunohistochemically localized in tubal ectopic pregnancy
Güney, M., Erdemoğlu, E., Oral, B., et al. (2008) · Acta Histochemica · doi:10.1016/j.acthis.2007.11.002 · Cited by 18 · see citing works
Singh, R., Graves, M. L., Roskelley, C. D., et al. (2007) · Domestic Animal Endocrinology · doi:10.1016/j.domaniend.2007.09.004 · Cited by 18 · see citing works
Pregnancy: Proliferative activity in ectopic trophoblastic tissue
Klein, M. U., Graf, A.-H., Hütter, W., et al. (1995) · Human Reproduction · doi:10.1093/oxfordjournals.humrep.a136315 · Cited by 16 · see citing works
Brown, J. K., Shaw, J. L. V., Critchley, H., et al. (2011) · Molecular Human Reproduction · doi:10.1093/molehr/gar068 · Cited by 16 · see citing works
DUELLO, T. M., Tsai, S., & Ess, P. J. V. (1993) · Endocrinology · doi:10.1210/endo.133.6.8243285 · Cited by 14 · see citing works
Nio‐Kobayashi, J., Abidin, H. B. Z., Brown, J. K., Iwanaga, T., Horne, A. W., & Duncan, W. C. (2014) · Cells Tissues Organs · doi:10.1159/000439166 · Cited by 9 · see citing works
Self-citationSengupta, A., & Sridaran, R. (2007) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.7a7289.2007 · Cited by 8 · see citing works
Sengupta, A., Baker, T. M., Chakrabarti, N., Whittaker, J. A., & Sridaran, R. (2007) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.6a7135.2007 · Cited by 6 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Klein M, Graf AH, Hutter W, Hacker GW, Beck A, Staudach A, et al. Prolifer- ative activity in ectopic trophoblastic tissue. Hum Reprod 1995;10:2441-4.
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Brown, J. L., Sones, J. L., Angulo, C. N., Abbott, K., Miller, A. D., Boehm, U., & Roberson, M. S. (2019) · Scientific Reports · doi:10.1038/s41598-019-45997-0 · Cited by 13 · see citing works
- Authors
- Peng, B., Klausen, C., Campbell, L., Leung, P. C. K., Horne, A. W., & Bedaiwy, M. A.
- Affiliations
- University of British Columbia (CA)
- MRC Centre for Reproductive Health (GB)
- Corresponding author
- Bedaiwy, Mohamed A.
- Venue
- Fertility and Sterility
- Publisher
- Elsevier BV
- Published
- 2016-06
- Volume
- 105
- Issue
- 6
- Pages
- 1620-1627.e3
- ISSN
- 0015-0282 (ISSN-L)
- OpenAlex
- W2296497816
- Cited by
- 15
- References
- 37
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2020: 4 · 2019: 2 · 2017: 1
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Fertility and Sterility (publishedVersion)
- PubMed (publishedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by-nd)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Health and Contraception
- Keywords
- gonadotropin-releasing hormone, GnRH receptor, tubal pregnancy, trophoblast, syncytiotrophoblast, cytotrophoblast, extravillous trophoblast, fallopian tube epithelium, GnRH signaling, GnRH antagonist, BeWo cells, HTR-8/SVneo cells
- MeSH terms
- Cell Line, Transformed, Cell Survival, Fallopian Tubes, Female, Humans, Gonadotropin-Releasing Hormone, Embryo Implantation, Pregnancy, Pregnancy, Tubal, Receptors, LHRH, Trophoblasts, Gene Expression Regulation, Developmental, Cell Line, Tumor
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