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Cited by 105Algal biology and biofuel productionFatty Acid Research and HealthSeaweed-derived Bioactive Compounds

The DOI matches its Crossref record; 28 of the 29 references it cites were found in a registry or an index.

Fractionation of lipids and purification of γ-linolenic acid (GLA) from Spirulina platensis

Sajilata, M.G., Singhal, R.S., Kamat, M.Y.·2008·Food Chemistry
DOI 10.1016/j.foodchem.2008.01.005
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Abstract

The microalgae, Spirulina platensis, is an excellent source of γ-linolenic acid (GLA), an essential polyunsaturated fatty acid and a potent nutraceutical. The fatty acid composition of S. platensis ARM 740 was determined after transmethylation by gas chromatography (GC). Lipid fractionation was achieved on silica gel column chromatography and preparative TLC. Neutral lipids, glycolipids and phospholipids accounted for 77.0%, 15.6% and 7.4%, respectively, of the total lipid fraction. S. platensis ARM 740 was found to contain 94% of the total GLA in the glycolipid fraction. Attempts were made to purify GLA methyl ester by using urea to form inclusion complexes with the saturated and the less unsaturated FAMEs (fatty acid methyl esters), which enhanced the purity of GLA methyl ester to 84%. A further approach to isolate GLA methyl ester with higher purity involved the use of argentated silica gel chromatography. An initial PUFA concentration step frequently adopted by most researchers to increase GLA purity was not necessary in the isolation of GLA from S. platensis. A GLA methyl ester with a purity of >96% and a recovery of 66% was obtained. Purity of the isolated GLA methyl ester was confirmed by GC and IR analysis with respect to authentic standard.

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Crossref · 10.1016/j.foodchem.2008.01.005

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  1. Takemoto, 1984, Inclusion compounds of urea, thiourea and selenourea, Vol. 2, 47

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Metadata

Authors
Sajilata, M. G., Singhal, R. S., & Kamat, M. Y.
Affiliations
Institute of Chemical Technology (IN)
Corresponding author
Singhal, R.S.
ORCID
Singhal, R.S.
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2008-08
Volume
109
Issue
3
Pages
580-586
ISSN
0308-8146 (ISSN-L)
DOI
10.1016/j.foodchem.2008.01.005
OpenAlex
W2097947545
Cited by
105
References
29
Citations by year
2026: 2 · 2025: 6 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 6 · 2020: 12 · 2019: 7 · 2018: 4 · 2017: 4 · 2016: 8 · 2015: 5 · 2014: 5 · 2013: 4 · 2012: 5
FWCI
3.98
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
Food Chemistry (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords
gamma-linolenic acid, Spirulina platensis, lipid fractionation, fatty acid composition, glycolipids, phospholipids, neutral lipids, microalgae, nutraceuticals, polyunsaturated fatty acids

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