Cited by 105Algal biology and biofuel productionFatty Acid Research and HealthSeaweed-derived Bioactive Compounds

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Fractionation of lipids and purification of γ-linolenic acid (GLA) from Spirulina platensis

Sajilata, M.G., Singhal, R.S., Kamat, M.Y.·2008·Food Chemistry

DOI 10.1016/j.foodchem.2008.01.005

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Abstract

The microalgae, Spirulina platensis, is an excellent source of γ-linolenic acid (GLA), an essential polyunsaturated fatty acid and a potent nutraceutical. The fatty acid composition of S. platensis ARM 740 was determined after transmethylation by gas chromatography (GC). Lipid fractionation was achieved on silica gel column chromatography and preparative TLC. Neutral lipids, glycolipids and phospholipids accounted for 77.0%, 15.6% and 7.4%, respectively, of the total lipid fraction. S. platensis ARM 740 was found to contain 94% of the total GLA in the glycolipid fraction. Attempts were made to purify GLA methyl ester by using urea to form inclusion complexes with the saturated and the less unsaturated FAMEs (fatty acid methyl esters), which enhanced the purity of GLA methyl ester to 84%. A further approach to isolate GLA methyl ester with higher purity involved the use of argentated silica gel chromatography. An initial PUFA concentration step frequently adopted by most researchers to increase GLA purity was not necessary in the isolation of GLA from S. platensis. A GLA methyl ester with a purity of >96% and a recovery of 66% was obtained. Purity of the isolated GLA methyl ester was confirmed by GC and IR analysis with respect to authentic standard.

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Authors: Sajilata, M. G., Singhal, R. S., & Kamat, M. Y.
Affiliations: Institute of Chemical Technology (IN)
Corresponding author: Singhal, R.S.
ORCID: Singhal, R.S.
Venue: Food Chemistry
Publisher: Elsevier BV
Published: 2008-08
Volume: 109
Issue: 3
Pages: 580-586
ISSN: 0308-8146 (ISSN-L)
DOI: 10.1016/j.foodchem.2008.01.005
OpenAlex: W2097947545
Cited by: 105
References: 29
Citations by year: 2026: 2 · 2025: 6 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 6 · 2020: 12 · 2019: 7 · 2018: 4 · 2017: 4 · 2016: 8 · 2015: 5 · 2014: 5 · 2013: 4 · 2012: 5
FWCI: 3.98
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Food Chemistry (publishedVersion)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords: gamma-linolenic acid, Spirulina platensis, lipid fractionation, fatty acid composition, glycolipids, phospholipids, neutral lipids, microalgae, nutraceuticals, polyunsaturated fatty acids

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Fractionation of lipids and purification of γ-linolenic acid (GLA) from Spirulina platensis

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