Characterisation of partially purified milk-clotting enzyme from Solanum dubium Fresen seeds

Ahmed, Isam Ali Mohamed; Morishima, Isao; Babiker, Elfadil E.; Mori, Nobuhiro

doi:10.1016/j.foodchem.2008.11.072

Cited by 100Enzyme Production and CharacterizationPineapple and bromelain studiesEnzyme Catalysis and Immobilization

Characterisation of partially purified milk-clotting enzyme from Solanum dubium Fresen seeds

Ahmed, Isam Ali Mohamed, Morishima, Isao, Babiker, Elfadil E., Mori, Nobuhiro·2009·Food Chemistry

DOI 10.1016/j.foodchem.2008.11.072

Open this paper in CoChat — free account

Abstract

The seeds of Solanum dubium were blended and extracted using different types of buffers. The most reliable, quick, and efficient buffer was found to be 5% NaCl in acetate buffer (pH 5.0) which was used throughout the study. The extract was filtered and fractionated twice with ammonium sulphate. The partially purified enzyme was characterised by SDS-PAGE which showed a band of molecular weight of 66kDa with the presence of other bands of low density. When compared with other plant enzymes, S. dubium enzyme was found to have higher clotting and proteolytic activities. The activity of the enzyme was steadily increased with enzyme and substrate concentration. The enzyme was found to be very stable against a wide range of pH values as well as a wide range of temperature (20-90°C). The results of substrate specificity of the enzyme showed that the partially purified enzyme preferred both hydrophilic and hydrophobic amino acid residues at P1 position. The catalytic efficiency of the purified enzyme was enhanced by an aliphatic amino acids (Leu) compared to aromatic residue (Phe) at P1 position at the same site.

Integrity signals

Registry

Crossref · 10.1016/j.foodchem.2008.11.072

Cites

20 of 20 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Ahmed et al. (2009a) found that milk-clotting activity of enzyme extract from Solanum dubium was 880 MCU and calf rennet was 2,496 MCU at 37 °C.
“Ahmed et al. (2009a) compared the milk-clotting activity of enzyme extract from Solanum dubium with calf rennet and found that the milk-clotting activity was 880 and 2,496 MCU at 37 °C, respectively.”
From Plant proteases as milk-clotting enzymes in cheesemaking: a review (2014) · cites this paper as Ahmed et al. (2009a)

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
Laemmli, U. K. (1970) · Nature · Cited by 5,673 · see citing works
The preparation and properties of two new chromogenic substrates of trypsin
Erlanger, B. F., Kokowsky, N., & Cohen, W. (1961) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(61)90145-x · Cited by 3,346 · see citing works
Serine Protease Mechanism and Specificity
Hedstrom, L. (2002) · Chemical Reviews · doi:10.1021/cr000033x · Cited by 1,841 · see citing works
Cheesemaking with vegetable coagulants—the use of <i>Cynara</i> L. for the production of ovine milk cheeses
Roseiro, L. B., Barbosa, M., Ames, J. M., & Wilbey, R. A. (2003) · International Journal of Dairy Technology · doi:10.1046/j.1471-0307.2003.00080.x · Cited by 200 · see citing works
Purification and partial characterization of milk clotting proteases from flowers of Cynara cardunculus
Heimgartner, U., Pietrzak, M., Geertsen, R., Brodelius, P., da Silva Figueiredo, A. C., & Pais, M. S. S. (1990) · Phytochemistry · doi:10.1016/0031-9422(90)80090-4 · Cited by 185 · see citing works
[30] Milk-clotting enzyme from Mucor pusillus var. Lindt
Arima, Κ., Yu, J., & Iwasaki, S. (1970) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(70)19033-1 · Cited by 170 · see citing works
The vegetable rennet of Cynara cardunculus L. contains two proteinases with chymosin and pepsin-like specificities
Ver�ssimo, P., Esteves, C., Faro, C., & Pires, E. (1995) · Biotechnology Letters · doi:10.1007/bf00129389 · Cited by 148 · see citing works
The use of the direct linear plot for determining initial velocities
Cornish-Bowden, A. J. (1975) · Biochemical Journal · doi:10.1042/bj1490305 · Cited by 124 · see citing works
Highly stable glycosylated serine protease from the medicinal plant Euphorbia milii
Yadav, S. C., Pande, M., & Jagannadham, M. V. (2006) · Phytochemistry · doi:10.1016/j.phytochem.2006.06.002 · Cited by 94 · see citing works
Purification and characterization of a serine protease from Cucumis trigonus Roxburghi
Asif-Ullah, M., Kim, K., & Yu, Y. G. (2006) · Phytochemistry · doi:10.1016/j.phytochem.2006.02.020 · Cited by 81 · see citing works
Purification of cynarases from artichoke (Cynara scolymus L.): enzymatic properties of cynarase A
Sidrach, L., García-Cánovas, F., Tudela, J., & Neptuno Rodríguez-López, J. (2005) · Phytochemistry · doi:10.1016/j.phytochem.2004.10.005 · Cited by 74 · see citing works
Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports
Lamas, E. M., Barros, R., Balcão, V. M., & Malcata, F. X. (2001) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(01)00308-8 · Cited by 73 · see citing works
SEP-1 - a subtilisin-like serine endopeptidase from germinated seeds of Hordeum vulgare L. cv. Morex
Fontanini, D., & Jones, B. L. (2002) · Planta · doi:10.1007/s00425-002-0823-4 · Cited by 48 · see citing works
Isolation and Characterization of a Possible Native Cucumisin from Developing Melon Fruits and Its Limited Autolysis to Cucumisin
Yamagata, H., Ueno, S., & Iwasaki, T. (1989) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1989.10869404 · Cited by 29 · see citing works
Proteases from the sarcocarp of yellow snake-gourd
Uchikoba, T., Horita, H., & Kaneda, M. (1990) · Phytochemistry · doi:10.1016/0031-9422(90)85032-b · Cited by 23 · see citing works
Cleavage specificity of cucumisin, a serine protease, with synthetic substrates
Arima, K., Yonezawa, H., Uchikoba, T., Shimada, M., & Kaneda, M. (2000) · Phytochemistry · doi:10.1016/s0031-9422(00)00157-6 · Cited by 18 · see citing works
Effect of an enzymatic complex from pineapple pulp on the primary clotting phase
Cattaneo, T. M. P., Nigro, F., Messina, G., & Giangiacomo, R. (1994) · Milk science international/Milchwissenschaft · Cited by 16 · see citing works
Milk Clotting Enzyme from Solanm Dobium
Yousif, B. H., McMahon, D. J., & Shammet, K. M. (1996) · Digital Commons - USU (Utah State University) · doi:10.1016/0958-6946(95)00025-9

Flagged first, then by citations

1 of the 21 references couldn’t be matched to a known paper.

Sorted by citations

Recent Advances in Milk Clotting Enzymes
JACOB, M., JAROS, D., & ROHM, H. (2011) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2010.00633.x · Cited by 295 · see citing works
Plant proteases as milk-clotting enzymes in cheesemaking: a review
Shah, M. A., Mir, S. A., & Paray, M. A. (2014) · Dairy Science & Technology · doi:10.1007/s13594-013-0144-3 · Cited by 209 · see citing works
Milk-clotting properties of plant rennets and their enzymatic, rheological, and sensory role in cheese making: A review
Amira, A. B., Besbes, S., Attia, H., & Blecker, C. S. (2017) · International Journal of Food Properties · doi:10.1080/10942912.2017.1289959 · Cited by 129 · see citing works
Biodiversity of Lactic Acid Bacteria
Liu, W., Pang, H., Zhang, H., & Cai, Y. (2014) · Springer eBooks · doi:10.1007/978-94-017-8841-0_2 · Cited by 119 · see citing works
Production of Plant Proteases and New Biotechnological Applications: An Updated Review
Troncoso, F. D., Sánchez, D. A., & Ferreira, M. L. (2022) · ChemistryOpen · doi:10.1002/open.202200017 · Cited by 127 · see citing works
Caseinolytic and milk-clotting activities from Moringa oleifera flowers
Pontual, E. V., Carvalho, B. E. A., Bezerra, R. S., Coelho, L. C. B. B., Napoleão, T. H., & Paiva, P. M. G. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2012.06.087 · Cited by 97 · see citing works
Plant Milk-Clotting Enzymes for Cheesemaking
Nicosia, F. D., Puglisi, I., Pino, A., Caggia, C., & Randazzo, C. L. (2022) · Foods · doi:10.3390/foods11060871 · Cited by 95 · see citing works
“0) as well as a wide range of temperature (20–90 ◦C) [62].”
Background
Purification and characterization of a milk-clotting aspartic protease from Withania coagulans fruit
Salehi, M., Aghamaali, M. R., Sajedi, R. H., Asghari, S. M., & Jorjani, E. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.02.034 · Cited by 74 · see citing works
Religiosin B, a milk-clotting serine protease from Ficus religiosa
Kumari, M., Sharma, A., & Jagannadham, M. V. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2011.09.122 · Cited by 69 · see citing works
Production and biochemical properties of proteases secreted by Aspergillus niger under solid state fermentation in response to different agroindustrial substrates
de Castro, R. J. S., Nishide, T. G., & Sato, H. H. (2014) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2014.06.001 · Cited by 67 · see citing works
Valorization, extraction optimization and technology advancements of artichoke biowastes: Food and non-food applications
Zayed, A., & Farag, M. A. (2020) · LWT · doi:10.1016/j.lwt.2020.109883 · Cited by 60 · see citing works
A new milk-clotting enzyme produced by Bacillus sp. P45 applied in cream cheese development
Lemes, A. C., Pavón, Y., Lazzaroni, S., Rozycki, S., Brandelli, A., & Kalil, S. J. (2016) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2015.10.038 · Cited by 52 · see citing works
Three phase partitioning, a scalable method for the purification and recovery of cucumisin, a milk-clotting enzyme, from the juice of Cucumis melo var . reticulatus
Gagaoua, M., Ziane, F., Rabah, S. N., Boucherba, N., Ait-Kaki, A., Bouanane‐Darenfed, A., & Hafid, K. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.04.060 · Cited by 50 · see citing works
Enzymatic milk clotting activity in artichoke (Cynara scolymus) leaves and alpine thistle (Carduus defloratus) flowers. Immobilization of alpine thistle aspartic protease
Esposito, M., Pierro, P. D., Dejonghe, W., Mariniello, L., & Porta, R. (2016) · Food Chemistry · doi:10.1016/j.foodchem.2016.02.060 · Cited by 48 · see citing works
Exploring the applications of plant-based coagulants in cheese production: A review
Mohsin, A. Z., Norsah, E., Marzlan, A. A., Rahim, M. H. A., & Hussin, A. S. M. (2023) · International Dairy Journal · doi:10.1016/j.idairyj.2023.105792 · Cited by 43 · see citing works
Characterisation of a milk-clotting extract from Balanites aegyptiaca fruit pulp
Beka, R. G., Krier, F., Botquin, M., Guiama, V. D., Donn, P., Libouga, D. G., Mbofung, C. M., Dimitrov, K., Slomianny, M., Guillochon, D., et al. (2013) · International Dairy Journal · doi:10.1016/j.idairyj.2013.06.013 · Cited by 42 · see citing works
Proteolytic and antioxidant activity of protein fractions of seeds of Cucurbita moschata
Dash, P., & Ghosh, G. (2016) · Food Bioscience · doi:10.1016/j.fbio.2016.12.004 · Cited by 42 · see citing works
Proteolysis and Rheological Properties of Cream Cheese Made with a Plant-Derived Coagulant from Solanum elaeagnifolium
Gutiérrez‐Méndez, N., Balderrama-Carmona, A., García-Sandoval, S. E., Ramírez-Vigil, P., Leal‐Ramos, M. Y., & García-Triana, A. (2019) · Foods · doi:10.3390/foods8020044 · Cited by 43 · see citing works
Effect of extraction pH on techno-functional properties of crude extracts from wild cardoon (Cynara cardunculus L.) flowers
Amira, A. B., Makhlouf-Gafsi, I., Petrut, R. F., Francis, F., Bauwens, J., Attia, H., Besbes, S., & Blecker, C. S. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.01.040 · Cited by 35 · see citing works
Characterization of partially purified milk‐clotting enzyme from sunflower (Helianthus annuus) seeds
Nasr, A. I. A. M., Ahmed, I. A. M., & Hamid, O. I. A. (2016) · Food Science & Nutrition · doi:10.1002/fsn3.338 · Cited by 35 · see citing works
Self-citation
High Milk-Clotting Activity Expressed by the Newly Isolated Paenibacillus spp. Strain BD3526
Hang, F., Liu, P., Wang, Q., Han, J., Wu, Z., Gao, C., Liu, Z., Zhang, H., & Chen, W. (2016) · Molecules · doi:10.3390/molecules21010073 · Cited by 34 · see citing works
Three phase partitioning to concentrate milk clotting proteases from Wrightia tinctoria R. Br and its characterization
Rajagopalan, A., & Sukumaran, B. O. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.06.042 · Cited by 35 · see citing works
Partial Characterization of the Proteolytic Properties of an Enzymatic Extract From “Aguama” Bromelia pinguin L. Fruit Grown in Mexico
Moreno-Hernández, J. M., Hernández-Mancillas, X. D., Coss-Navarrete, E. L., Mazorra-Manzano, M. A., Osuna‐Ruíz, I., Rodríguez-Tirado, V. A., & Salazar‐Leyva, J. A. (2016) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-016-2319-x · Cited by 31 · see citing works
Utility of Milk Coagulant Enzyme of Moringa oleifera Seed in Cheese Production from Soy and Skim Milks
Sánchez-Muñoz, M. A., Valdez-Solana, M. A., Avitia-Domínguez, C. I., Ramírez-Baca, P., Candelas-Cadillo, M. G., Aguilera-Ortíz, M., Meza-Velázquez, J. A., Téllez‐Valencia, A., & Sierra‐Campos, E. (2017) · Foods · doi:10.3390/foods6080062 · Cited by 29 · see citing works
Purification and characterization of a novel milk-clotting metalloproteinase from Paenibacillus spp. BD3526
Hang, F., Wang, Q., Hong, Q., Liu, P., Wu, Z., Liu, Z., Zhang, H., & Chen, W. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.01.028 · Cited by 27 · see citing works
Biochemical and rheological characterization of a protease from fruits of Withania coagulans with a milk-clotting activity
Beigomi, M., Mohammadifar, M. A., Hashemi, M., rohani, M. G., Senthil, K., & Valizadeh, M. (2014) · Food Science and Biotechnology · doi:10.1007/s10068-014-0247-5 · Cited by 27 · see citing works
Proteomic analysis of Moringa oleifera Lam. leaf extract provides insights into milk-clotting proteases
Shi, Y., Prabakusuma, A. S., Zhao, Q., Wang, X., & Huang, A. (2019) · LWT · doi:10.1016/j.lwt.2019.04.035 · Cited by 26 · see citing works
Milk-Clotting Plant Proteases for Cheesemaking
Mazorra-Manzano, M. A., Moreno-Hernández, J. M., & Ramírez-Suarez, J. C. (2018) · Biotechnological Applications of Plant Proteolytic Enzymes · doi:10.1007/978-3-319-97132-2_2 · Cited by 28 · see citing works
Plant latex as vegetable source for milk clotting enzymes and their use in cheese preparation
Mahajan, R. T., & Chaudhari, G. (2014) · Cited by 25 · see citing works
Efficient valorization of feather waste by Bacillus cereus IIPK35 for concomitant production of antioxidant keratin hydrolysate and milk-clotting metallo-serine keratinase
Jana, A., Kakkar, N., Halder, S. K., Das, A. J., Bhaskar, T., Ray, A., & Ghosh, D. (2022) · Journal of Environmental Management · doi:10.1016/j.jenvman.2022.116380 · Cited by 25 · see citing works
Protease activity of enzyme extracts from tamarillo fruit and their specific hydrolysis of bovine caseins
Zhao, L., Scott, K., Hémar, Y., & Otter, D. E. (2018) · Food Research International · doi:10.1016/j.foodres.2018.04.039 · Cited by 25 · see citing works
Religiosin C, a cucumisin-like serine protease from Ficus religiosa
Sharma, A., Kumari, M., & Jagannadham, M. V. (2012) · Process Biochemistry · doi:10.1016/j.procbio.2012.02.015 · Cited by 23 · see citing works
Comparison of buffalo cottage cheese made from aqueous extract of W ithania coagulans with commercial calf rennet
Khan, R. S., & Masud, T. (2013) · International Journal of Dairy Technology · doi:10.1111/1471-0307.12048 · Cited by 23 · see citing works
Purification and characterization of Bacillussubtilis milk-clotting enzyme from Tibet Plateau and its potential use in yak dairy industry
Li, Y., Liang, S., Zhi, D.-J., Chen, P., Su, F., & Li, H.-Y. (2012) · European Food Research and Technology · doi:10.1007/s00217-012-1663-5 · Cited by 22 · see citing works
Milk‐clotting enzymes produced by Aspergillus flavo furcatis strains on Amazonic fruit waste
Alecrim, M. M., Palheta, R. A., Teixeira, M. F. S., & Oliveira, I. M. D. A. (2014) · International Journal of Food Science & Technology · doi:10.1111/ijfs.12677 · Cited by 22 · see citing works
Peynir Üretimi İçin Sütü Pıhtılaştıran Enzimlere Genel Bir Bakış ve Güncel Gelişmeler
Çakmakçı, S., Cantürk, A., & Çakır, Y. (2017) · Akademik Gıda · doi:10.24323/akademik-gida.370264 · Cited by 21 · see citing works
Purification and thermodynamic characterization of acid protease with novel properties from Melilotus indicus leaves
Zaman, U., Khan, S. U., Alem, S. F. M., Rehman, K. U., Almehizia, A. A., Naglah, A. M., Al‐Wasidi, A. S., Refat, M. S. E.-D., Saeed, S., & Zaki, M. E. A. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.123217 · Cited by 21 · see citing works
PARTIAL PURIFICATION OF MILK-CLOTTING ENZYME FROM THE SEEDS OF MORINGA OLEIFERA
Tajalsir, A. E., Ebraheem, A. S. A., Abdallah, A. M., Khider, F. J., Elsamani, M. O., & Ahmed, I. A. M. (2014) · Journal of Microbiology Biotechnology and Food Sciences · doi:10.15414/jmbfs.2014.4.1.58-62 · Cited by 21 · see citing works
Self-citation
Milk-clotting mechanism of Dregea sinensis Hemsl. protease
Zhang, Y., Wang, H., Tao, L., & Huang, A. (2015) · Journal of Dairy Science · doi:10.3168/jds.2015-9851 · Cited by 20 · see citing works
Comparison of Quality of Sudanese White Cheese (Gibna bayda) Manufactured with Solanum dubium Fruit Extract and Rennet
Kheir, S. E. O., Osman Ali Osman El, O., & Ab, M. O. M. (2011) · Pakistan Journal of Nutrition · doi:10.3923/pjn.2011.106.111 · Cited by 20 · see citing works
Surface display of acid protease on the cells of Yarrowia lipolytica for milk clotting
Yu, X., Madzak, C., Li, H., Chi, Z., & Li, J. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2549-8 · Cited by 19 · see citing works
Kinetic and thermodynamic study of aspartic protease extracted from Withania coagulans
Ahmadi, S., Salehi, M., & Ausi, S. (2020) · International Dairy Journal · doi:10.1016/j.idairyj.2020.104960 · Cited by 17 · see citing works
Plant Proteases in Food Processing
Shah, M. A., & Mir, S. A. (2019) · Reference series in phytochemistry · doi:10.1007/978-3-319-78030-6_68 · Cited by 17 · see citing works
Utility of Moringa oleifera waste as a coagulant in goat soft cheese production
Abdeen, E.-S. M., Ibrahim, O. A. E.-H., & Kholif, A. M. M. (2021) · Heliyon · doi:10.1016/j.heliyon.2021.e07536 · Cited by 16 · see citing works
Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent
da Silva, A. C., Nascimento, T. C. E. D. S., da Silva, S. A., Herculano, P. N., & Moreira, K. A. (2013) · Food Science and Technology · doi:10.1590/s0101-20612013005000075 · Cited by 16 · see citing works
Plant-derived rennet: research progress, novel strategies for their isolation, identification, mechanism, bioactive peptide generation, and application in cheese manufacturing
Zhang, X.-T., Tao, L., Wei, G.-Q., Yang, M., Wang, Z.-L., Shi, C.-Y., Shi, Y.-N., & Huang, A.-X. (2023) · Critical reviews in food science and nutrition · doi:10.1080/10408398.2023.2275295 · Cited by 17 · see citing works
Proteomics analysis of the bio-functions of Dregea sinensis stems provides insights regarding milk-clotting enzyme
Zhao, Q., Zhao, C., Shi, Y., Wei, G., Yang, K., Wang, X., & Huang, A. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110340 · Cited by 13 · see citing works
Use of caprifig tree extract as a substitute for calf rennet in goat’s fresh cheese production
Hachana, Y., Aloui, O., & Fortina, R. (2021) · Small Ruminant Research · doi:10.1016/j.smallrumres.2021.106382 · Cited by 13 · see citing works
Application of actinidin as coagulants to produce Iranian white brined cheese: investigating the technological, textural, and sensorial properties
Dehkordi, A. M., Bari, M. R., Babaie, G., & Amiri, S. (2021) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-021-01219-8 · Cited by 12 · see citing works
New Coagulant Proteases for Cheesemaking from Leaves and Latex of the Spontaneous Plant Pergularia tomentosa: Biochemical Characterization of Coagulants and Sensorial Evaluation of Cheese
Leulmi, I., Zidoune, M. N., Hafid, K., Djeghim, F., Bourekoua, H., Dziki, D., & Różyło, R. (2023) · Foods · doi:10.3390/foods12132467 · Cited by 12 · see citing works
Isolation and identification of a fungal strain QY229 producing milk-clotting enzyme
Zhang, Z., Wang, C., Yao, Z., Zhao, J., Lü, F., Yu, G., Lan, W., & Lu, Z. (2011) · European Food Research and Technology · doi:10.1007/s00217-011-1454-4 · Cited by 11 · see citing works
Clotting Activities of Partially Purified Extracts of Moringa oleifera L. on Dromedary Camel Milk
Terefe, M. A., Kebede, A., & Kebede, M. (2018) · National Academic Digital Repository of Ethiopia · doi:10.20372/eajs.v11i2.407 · Cited by 11 · see citing works
Milk clotting and proteolytic activity of enzyme preparation from Pediococcus acidilactici SH for dairy products
Mohamed, M. I., Zaiton, H., Mohamed, M. A., Brahim, M. E., & Belal, J. M. (2015) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb2014.13974 · Cited by 11 · see citing works
Evidence of structural changes of an enzymatic extract entrapped into alginate beads
Illanes, C. O., Quiroga, E., Camí, G., & Ochoa, N. A. (2012) · Biochemical Engineering Journal · doi:10.1016/j.bej.2012.07.026 · Cited by 10 · see citing works
INFLUENCE OF DREGEA SINENSIS HEMSL. PROTEASE ON THE QUALITY OF MOZZARELLA CHEESE FROM BUFFALO MILK
Wang, H., Wang, Y., & Huang, A. (2017) · Emirates Journal of Food and Agriculture · doi:10.9755/ejfa.2016-09-1227 · Cited by 10 · see citing works
Future Perspective and Technological Innovation in Cheese Making Using Artichoke (Cynara scolymus) as Vegetable Rennet: A Review
Bolívar, M. S. B., Pasini, F., Marzocchi, S., Ravagli, C., & Tedeschi, P. (2023) · Foods · doi:10.3390/foods12163032 · Cited by 9 · see citing works
Structural insight into a novel neutral metalloproteinase from Paenibacillus spp. BD3526: Implications for mechanisms of rapid inactivation and calcium-dependent stability
Hang, F., Wang, Q., Hong, Q., Gao, C., Zhang, H., & Chen, W. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.10.098 · Cited by 9 · see citing works
Efficient three phase partitioning of actinidin from kiwifruit ( Actinidia deliciosa) and its characterization
Maskey, B., & Karki, D. B. (2023) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2023.2209877 · Cited by 8 · see citing works
Identification, structure, and caseinolytic properties of milk-clotting proteases from Moringa oleifera flowers
Zhao, Q., Huang, A., Wu, G., Guo, Q., Li, M., & Wang, X. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111598 · Cited by 8 · see citing works
Ficus palmata Forskål (beles adgi) as a source of milk clotting agent: a preliminary research
Sbhatu, D. B., Tekle, H. T., & Haddish, K. (2020) · BMC Research Notes · doi:10.1186/s13104-020-05293-x · Cited by 7 · see citing works
Purification and Characterization of Milk Clotting Enzyme from Edible Mushroom (Pleurotus florida)
(2021) · Letters in Applied NanoBioScience · doi:10.33263/lianbs112.33623373 · Cited by 7 · see citing works
Milk-Gelling Properties of Proteases Extracted from the Fruits of Solanum Elaeagnifolium Cavanilles
Nájera‐Domínguez, C., Gutiérrez‐Méndez, N., Carballo, D. E., Peralta-Pérez, M. D. R., Sánchez‐Ramírez, B., Nevárez‐Moorillón, G. V., Quintero‐Ramos, A., García-Triana, A., & Delgado, E. (2022) · International Journal of Food Science · doi:10.1155/2022/4625959 · Cited by 7 · see citing works
Usefulness of Moringa oleifera seed extract as Coagulant for the production of fresh camel cheese
Fguiri, I., Sboui, A., Yousfi, N., Arroum, S., Dbara, M., Hammadi, M., & Khorchani, T. (2024) · Italian Journal of Food Science · doi:10.15586/ijfs.v36i3.2517 · Cited by 5 · see citing works
Proteomics-guided isolation of a novel serine protease with milk-clotting activity from tamarillo (Solanum betaceum Cav.)
Zhang, T., Yang, Z., Zhang, Y., Yi, L., Duan, F., Zhao, Q., Gu, Y., & Wang, S. (2024) · Food Chemistry · doi:10.1016/j.foodchem.2024.141956 · Cited by 5 · see citing works
Effects of different coagulant enzymes used on quality of traditional Örgü cheese (Braided cheese)
Çelebi, M. (2015) · Mljekarstvo/Mljekarstvo.com · doi:10.15567/mljekarstvo.2015.0108 · Cited by 5 · see citing works
Purification and characterization of a novel milk-clotting enzyme from Brassica napus seeds.
Elsayed, S., & Azzouny, R. A. A. (2013) · AUSTRALIAN JOURNAL OF BASIC AND APPLIED SCIENCES · Cited by 5 · see citing works
Peptidases used in dairy technology: Current knowledge and relevant applications
Paiva, V. N., Martins, E., Machado, S. G., & de Carvalho, A. F. (2022) · Research Society and Development · doi:10.33448/rsd-v11i7.30367 · Cited by 4 · see citing works
CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.
Guzmán-Ceferino, J., Cobos-Puc, L. E., Rivera, C. A. S., Contreras‐Esquivel, J. C., Durán-Mendoza, T., & Belmares., S. Y. S. (2019) · Polibotánica · doi:10.18387/polibotanica.47.10 · Cited by 4 · see citing works
Performance of Solanum incunum Linnaeus as natural coagulant and disinfectant for drinking water
Kihampa, C., Mwegoha, W. J. S., Kaseva, M. E., & Marobhe, N. J. (2011) · African Journal of Environmental Science and Technology · doi:10.4314/ajest.v5i10.72092 · Cited by 4 · see citing works
EXTRACTION, PARTIAL PURIFICATION AND SOME FACTORS AFFECTING MILK CLOTTING ACTIVITY OF A MILK COAGULANT PREPARED FROM SUNFLOWER (Helianthus annus) SEEDS
Ramadan, I., El-Wahed, E. A., Rabie, A., & Baky, A. A. (2019) · Zagazig Journal of Agricultural Research · doi:10.21608/zjar.2019.33399 · Cited by 4 · see citing works
THE INVESTIGATION OF THE POTENTIONAL COMPLEX FROM PLANTAGO MAJOR TO COAGULATE MILK PROTEINS
Grek, O., Krasulya, O., Chubenko, L., & Tymchuk, A. (2018) · Institutional Repository of National University of Food Technologies · Cited by 3 · see citing works
Proteolytic, milk-clotting, and stability properties of proteases from Solanum elaeagnifolium fruit
Teutle-Paredes, J. G., Mazorra-Manzano, M. A., Martínez‐Porchas, M., Ramírez‐Suárez, J. C., Vallejo‐Córdoba, B., Torres‐Llanez, M. J., & González-Córdova, A. F. (2024) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2024.103068 · Cited by 3 · see citing works
Milk-clotting proteases from Onopordum macracanthum flowers: purification, characterization, and potential applications in cheesemaking
Zikiou, A., Saci, F., Fiala, S., & Dahdah, K. (2025) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-025-03297-4 · Cited by 3 · see citing works
Proteolytic and Milk Clotting Activity of Fractionated Protein of Lagenaria siceraria
Dash, P., Maharana, L., & Ghosh, G. (2015) · Journal of Applied Pharmaceutical Science · doi:10.7324/japs.2015.501214 · Cited by 3 · see citing works
ENZIMAS VEGETAIS: EXTRAÇÃO E APLICAÇÕES BIOTECNOLÓGICAS
Antunes, R. S., Lopes, F. M., Brito, A. O., Garcia, L. F., de Sousa, D. F., & Gil, E. D. S. (2017) · Infarma - Ciências Farmacêuticas · doi:10.14450/2318-9312.v29.e3.a2017.pp181-198 · Cited by 3 · see citing works
Preparation and Evaluation of Cottage Cheese using Enzyme Extracted from Mustard Oilseed Cake
Jain, S., Gupta, S., Kumar, A., Bangar, Y., & Ahlawat, S. S. (2019) · International Journal of Current Microbiology and Applied Sciences · doi:10.20546/ijcmas.2019.812.148 · Cited by 2 · see citing works
Plant Proteases in Food Processing
Shah, M. A., & Mir, S. A. (2018) · Reference series in phytochemistry · doi:10.1007/978-3-319-54528-8_68-1 · Cited by 2 · see citing works
Use of Proteolytic Activity of Ficus carica in Milk Coagulation
Priyashantha, H., Ranadheera, C. S., Senadheera, T. R. L., Hettiarachchi, H. T. M., Jayarathna, S., & Vidanarachchi, J. K. (2023) · Springer eBooks · doi:10.1007/978-3-031-16493-4_33 · Cited by 2 · see citing works
Screening and isolation of milk-clotting enzymes from seaweed
Arbita, A. A., Paul, N. A., Cox, J. M., & Zhao, J. J. (2023) · Journal of Applied Phycology · doi:10.1007/s10811-023-02975-4 · Cited by 2 · see citing works
Milk-Coagulating Extract Produced from Solanum aethiopicum Shum Fruits: Multivariate Techniques of Preparation, Thermal Stability and Effect on Milk Solids Recovery in Curd
Guiama, V. D., Beka, R. G., Ngah, E., Libouga, D. G., Vercaigne‐Marko, D., & Mbofung, C. M. (2014) · International Journal of Food Engineering · doi:10.1515/ijfe-2012-0214 · Cited by 2 · see citing works
CHARACTERIZATION OF MILK CLOTTING PROPERTIES OF ADULT CATTLE RENNET MODIFIED WITH Moringa oleifera SEEDS AND ITS SUITABILITY AS CALF RENNET ALTERNATIVE IN CHEESE MAKING
Ismail, H. A. (2019) · Zagazig Journal of Agricultural Research · doi:10.21608/zjar.2019.40962 · Cited by 2 · see citing works
Genome wide identification of bromelain-like cysteine proteases in Puya raimondiiPreprint
Cabrera, I. C., Hernández, M., Chandía, D. R., Dagnino‐Leone, J., & Reyes, M. F. (2024) · Research Square · doi:10.21203/rs.3.rs-3848808/v1 · Cited by 2 · see citing works
Evaluation of Minerals Content in SolaniumDubium Collected From White Nilein Central Sudan
Mohammed, A. M. E. (2017) · IOSR Journal of Applied Chemistry · doi:10.9790/5736-1002010111 · Cited by 1 · see citing works
QUALITY AND PROPERTIES EVALUATION OF WHITE SOFT CHEESE MADE BY FREE OR IMMOBILIZED COAGULANT ENZYMES DURING STORAGE
(2021) · Egyptian Journal of Applied Science · doi:10.21608/ejas.2021.195264 · Cited by 1 · see citing works
Exploring the Antibiotic Potential of a Serine Protease from Solanum trilobatum Against Staphylococcus aureus Biofilms
Radhakrishnan, M., Balu, K. E., Lakshminarayanan, K., Nagampalli, R. S. K., Nadendla, E. K., & Gunasekaran, K. (2025) · Infectious Disease Reports · doi:10.3390/idr17030050 · Cited by 1 · see citing works
Chymosin, Pepsins, and Other Aspartyl Proteinases: Structures, Functions, Catalytic Mechanism and Milk-Clotting Properties
Uniacke‐Lowe, T., & Fox, P. F. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-15956-5.00027-0 · Cited by 1 · see citing works
Caseinolytic Mechanism of Serine Protease MCP746 from Tamarillo ( Solanum betacea Cav.) and Potential Application in Fresh Cheese
Zhang, T., Bao, X., Yang, S., Lu, C., Zhao, Q., Gu, Y., & Wang, S. (2025) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5c04993 · Cited by 1 · see citing works
HIDROLISIS DE PROTEÍNAS POR UN EXTRACTO ENZIMÁTICO DE FLOR DE NARANJO AGRIO (Citrus aurantium) Y SU POTENCIAL COAGULANTE PARA PRODUCCIÓN DE QUESOS
Mazorra-Manzano, M. A., Moreno-Hernández, J. M., Torres‐Llanez, M. J., Ramírez‐Suárez, J. C., González-Córdova, A. F., & Vallejo‐Córdoba, B. (2013) · BIOtecnia · doi:10.18633/bt.v15i3.155 · Cited by 1 · see citing works
Effectiveness of Fe tablets on anemia and its relationship with nutritional status and learning outcomes
Atikah, N., Fardani, R. A., Idawati, S., Pertiwi, A. D., Aini, A., Sukmana, D. J., Utami, E. F., Setiyani, R. P. I., & Cahyadin, A. (2023) · AIP conference proceedings · doi:10.1063/5.0124523 · Cited by 1 · see citing works
Elaboración de queso tipo panela con extracto de hoja de ortiga y su comparación de parámetros fisicoquimicos
González-Lemus, U., Lira, A. Q., Piloni‐Martini, J., Campos-Montiel, R. G., Reyes-Munguía, A., Martínez-Trujillo, M. A., & Vera, N. G. (2019) · Boletín de Ciencias Agropecuarias del ICAP · doi:10.29057/icap.v5i9.3787
PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS
Dash, P., & Ghosh, G. (2015) · International Journal of Pharmacy and Pharmaceutical Sciences
Analisis Hubungan Kekerabatan Fenetik Famili Euphorbiaceae di Kota Salatiga berdasarkan Karakter Morfologi serta Sifat Kimia
Prasgi, H. C., & Kasmiyati, S. (2022) · Jurnal Biologi Indonesia · doi:10.47349/jbi/18012022/67
Initial characterization of potential proteolytic enzyme in crude extract of terong para Solanum virginianum
Saputri, D. S., Mikhratunnisa, Yanti, S., Rosmayana, A., Insani, S. R., Lestari, M., & Walfadiah, N. (2022) · AIP conference proceedings · doi:10.1063/5.0104031
Development of a plant-based milk-clotting enzyme and a debittering microbial culture for the formulation of halal-certified healthy cheese
Nicosia, F. D. (2024) · doi:10.47328/ufvbbt.2024.152
Beef Tenderizing Potential of Solanum dubium (Gubbain) protease
Biraima, A. D. A., & Webb, E. C. (2025) · Meat and Muscle Biology · doi:10.22175/mmb.18980
Comparative Quality Assessment of Sudanese White Cheese Coagulated With Acacia nubica Bark Extract and Rennet
Gadein, K. A., Mustafa, S. E., Babiker, W. A. M., & Abuagla, M. A. A. (2025) · Journal of Food Quality · doi:10.1155/jfq/7868250
Sycamore fig (Ficus sycomorus) fruit-derived Milk-clotting enzyme as a partial rennet substitute: Effects on physicochemical, textural, and sensory properties of soft white cheese
Elkot, W. F., Ismail, H. A., Alsulami, T. S., Mostafa, M. S., Althawab, S. A., Alohali, B. M., Abu-Taha, H. L., Al-Farga, A., & Elmahdy, A. M. (2026) · Food Research International · doi:10.1016/j.foodres.2026.119015
Phytochemical Profile and Pharmacological Potentials of Solanum dubium (Fresen.)
Osman, W., Mirghani, M., Kosar, A. M., Mohamed, F. I., Khadijah, K., Alqarni, M. H., Foudah, A. I., Ashour, A. A., Aljarba, T. M., & Samra, R. M. (2026) · Natural Resources for Human Health · doi:10.53365/nrfhh/224632
Plant peptidases: applications, challenges and perspectives in the food industry sectors
Santos, P. T. M., Salgado, C. A., Fernandes, F. J. N., Matos, Y. A., Machado, S. G., Randazzo, C. L., & de Carvalho, A. F. (2026) · Ciência Rural · doi:10.1590/0103-8478cr20250356

Isolation, Purification and Partial Characterization of a Serine-Like Protease from Solanum dubium Seeds
Abbas, F. M., & Abdelrahman, A. E. (2021) · Biochemistry & Molecular Biology Journal · doi:10.36648/2471-8084.21.7.102 · Cited by 2 · see citing works
pH stability and influence of salts on activity of a milk-clotting enzyme from Solanum dubium seeds and its enzymatic action on bovine caseins
Mohamed Ahmed, I. A., Babiker, E. E., & Mori, N. (2010) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2009.12.011 · Cited by 62 · see citing works
Self-citation
Dubiumin, a chymotrypsin-like serine protease from the seeds of Solanum dubium Fresen
Mohamed Ahmed, I. A., Morishima, I., Babiker, E. E., & Mori, N. (2009) · Phytochemistry · doi:10.1016/j.phytochem.2009.01.016 · Cited by 71 · see citing works
Self-citation
Milk Clotting Enzyme from Solanm Dobium
Yousif, B. H., McMahon, D. J., & Shammet, K. M. (1996) · Digital Commons - USU (Utah State University) · doi:10.1016/0958-6946(95)00025-9
Characterization of partially purified milk‐clotting enzyme from sunflower (Helianthus annuus) seeds
Nasr, A. I. A. M., Ahmed, I. A. M., & Hamid, O. I. A. (2016) · Food Science & Nutrition · doi:10.1002/fsn3.338 · Cited by 35 · see citing works
Self-citation

Authors: Ahmed, I. A. M., Morishima, I., Babiker, E. E., & Mori, N.
Affiliations: Tottori University (JP)
ORCID: Ahmed, Isam Ali Mohamed
Venue: Food Chemistry
Publisher: Elsevier BV
Published: 2009-09
Volume: 116
Issue: 2
Pages: 395-400
ISSN: 0308-8146 (ISSN-L)
DOI: 10.1016/j.foodchem.2008.11.072
OpenAlex: W2037441195
Cited by: 100
References: 21
Citations by year: 2026: 3 · 2025: 6 · 2024: 5 · 2023: 9 · 2022: 8 · 2021: 6 · 2020: 3 · 2019: 8 · 2018: 6 · 2017: 10 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 6 · 2012: 4
FWCI: 1.54
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Food Chemistry (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pineapple and bromelain studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords: milk-clotting enzyme, seeds, proteolytic activity, enzyme purification, ammonium sulfate precipitation, substrate specificity, catalytic efficiency, thermal stability, pH stability, molecular weight

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Characterisation of partially purified milk-clotting enzyme from Solanum dubium Fresen seeds

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks