Caseinolytic and milk-clotting activities from Moringa oleifera flowers

Pontual, Emmanuel V.; Carvalho, Belany E.A.; Bezerra, Ranilson S.; Coelho, Luana C.B.B.; Napoleão, Thiago H.; Paiva, Patrícia M.G.

doi:10.1016/j.foodchem.2012.06.087

Cited by 97Moringa oleifera research and applicationsEnzyme Production and CharacterizationProteins in Food Systems

Caseinolytic and milk-clotting activities from Moringa oleifera flowers

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Pontual, Emmanuel V., Carvalho, Belany E.A., Bezerra, Ranilson S., Coelho, Luana C.B.B., Napoleão, Thiago H., Paiva, Patrícia M.G.·2012·Food Chemistry

DOI 10.1016/j.foodchem.2012.06.087

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Abstract

This work reports the detection and characterization of caseinolytic and milk-clotting activities from Moringa oleifera flowers. Proteins extracted from flowers were precipitated with 60% ammonium sulphate. Caseinolytic activity of the precipitated protein fraction (PP) was assessed using azocasein, as well as α(s)-, β- and κ-caseins as substrates. Milk-clotting activity was analysed using skim milk. The effects of heating (30-100°C) and pH (3.0-11.0) on enzyme activities were determined. Highest caseinolytic activity on azocasein was detected after previous incubation of PP at pH 4.0 and after heating at 50°C. Milk-clotting activity, detected only in the presence of CaCl(2), was highest at incubation of PP at pH 3.0 and remained stable up to 50°C. The pre-treatment of milk at 70°C resulted in highest clotting activity. Enzyme assays in presence of protease inhibitors indicated the presence of aspartic, cysteine, serine and metallo proteases. Aspartic proteases appear to be the main enzymes involved in milk-clotting activity. PP promoted extensive cleavage of κ-casein and low level of α(s)- and β-caseins hydrolysis. The milk-clotting activity indicates the application of M. oleifera flowers in dairy industry.

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Uniacke‐Lowe, T., & Fox, P. F. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-417012-4.00004-1 · Cited by 34 · see citing works
Utility of Milk Coagulant Enzyme of Moringa oleifera Seed in Cheese Production from Soy and Skim Milks
Sánchez-Muñoz, M. A., Valdez-Solana, M. A., Avitia-Domínguez, C. I., Ramírez-Baca, P., Candelas-Cadillo, M. G., Aguilera-Ortíz, M., Meza-Velázquez, J. A., Téllez‐Valencia, A., & Sierra‐Campos, E. (2017) · Foods · doi:10.3390/foods6080062 · Cited by 29 · see citing works
“In addition, the MCA/PA ratio (2995:1) for seed extract was observed, and it was much higher than that reported in most previous studies [33,34].”
BackgroundResult
Impact of gastric coagulation on the kinetics of release of fat globules from milk of different species
Roy, D., Ye, A., Moughan, P. J., & Singh, H. (2021) · Food & Function · doi:10.1039/d0fo02870c · Cited by 28 · see citing works
Proteomic analysis of Moringa oleifera Lam. leaf extract provides insights into milk-clotting proteases
Shi, Y., Prabakusuma, A. S., Zhao, Q., Wang, X., & Huang, A. (2019) · LWT · doi:10.1016/j.lwt.2019.04.035 · Cited by 26 · see citing works
Milk-Clotting Plant Proteases for Cheesemaking
Mazorra-Manzano, M. A., Moreno-Hernández, J. M., & Ramírez-Suarez, J. C. (2018) · Biotechnological Applications of Plant Proteolytic Enzymes · doi:10.1007/978-3-319-97132-2_2 · Cited by 28 · see citing works
Clotting Properties of Onopordum tauricum (Willd.) Aqueous Extract in Milk of Different Species
Mozzon, M., Foligni, R., Mannozzi, C., Zamporlini, F., Raffaelli, N., & Aquilanti, L. (2020) · Foods · doi:10.3390/foods9060692 · Cited by 24 · see citing works
Purification and thermodynamic characterization of acid protease with novel properties from Melilotus indicus leaves
Zaman, U., Khan, S. U., Alem, S. F. M., Rehman, K. U., Almehizia, A. A., Naglah, A. M., Al‐Wasidi, A. S., Refat, M. S. E.-D., Saeed, S., & Zaki, M. E. A. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.123217 · Cited by 21 · see citing works
Comparative proteome analysis of matured dry and germinating Moringa oleifera seeds provides insights into protease activity during germination
Wang, X., Shi, Y., Chen, Y., Fan, J., Pu, Y., & Huang, A. (2020) · Food Research International · doi:10.1016/j.foodres.2020.109332 · Cited by 21 · see citing works
PARTIAL PURIFICATION OF MILK-CLOTTING ENZYME FROM THE SEEDS OF MORINGA OLEIFERA
Tajalsir, A. E., Ebraheem, A. S. A., Abdallah, A. M., Khider, F. J., Elsamani, M. O., & Ahmed, I. A. M. (2014) · Journal of Microbiology Biotechnology and Food Sciences · doi:10.15414/jmbfs.2014.4.1.58-62 · Cited by 21 · see citing works
Determination and characterisation of milk-clotting activity of two Solanum tuberosum aspartic proteases (StAPs)
Tito, F. R., Pepe, A., Tonón, C. V., Daleo, G. R., & Guevara, M. G. (2020) · International Dairy Journal · doi:10.1016/j.idairyj.2020.104645 · Cited by 20 · see citing works
Kinetic and thermodynamic study of aspartic protease extracted from Withania coagulans
Ahmadi, S., Salehi, M., & Ausi, S. (2020) · International Dairy Journal · doi:10.1016/j.idairyj.2020.104960 · Cited by 17 · see citing works
Plant Proteases in Food Processing
Shah, M. A., & Mir, S. A. (2019) · Reference series in phytochemistry · doi:10.1007/978-3-319-78030-6_68 · Cited by 17 · see citing works
Utility of Moringa oleifera waste as a coagulant in goat soft cheese production
Abdeen, E.-S. M., Ibrahim, O. A. E.-H., & Kholif, A. M. M. (2021) · Heliyon · doi:10.1016/j.heliyon.2021.e07536 · Cited by 16 · see citing works
“These findings are agreement with MCE from various plants has been reported (Mohamed Ahmed et al., 2009; Mazorra-Manzano et al., 2013; Pontual et al., 2012; Abd El-Salam et al., 2017).”
BackgroundResult
Exogenous enzymes in dairy technology: acidic proteases from processing discards of shrimp Pleoticus muelleri and their use as milk‐clotting enzymes for cheese manufacture
Pereira, N. D. L. Á., & Fernández-Giménez, A. V. (2016) · International Journal of Food Science & Technology · doi:10.1111/ijfs.13285 · Cited by 16 · see citing works
“Curd is formed due to associations between these proteins and calcium (Pontual et al., 2012).”
Background
Purification and characterization of a protease from the visceral mass of Mytella charruana and its evaluation to obtain antimicrobial peptides
Dornelles, L. P., de Souza, M. D. F. D., da Silva, P. M., Procópio, T. F., Filho, R. S. R., Lima, T. D. A., de Oliveira, A. P. S., Zingali, R. B., Paiva, P. M. G., Pontual, E. V., et al. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.11.044 · Cited by 16 · see citing works
Self-citation
Mitochondrial polymorphisms contribute to aging phenotypes in MNX mouse models
Vivian, C. J., Hagedorn, T. M., Jensen, R. A., Brinker, A. E., & Welch, D. R. (2018) · Cancer and Metastasis Reviews · doi:10.1007/s10555-018-9773-6 · Cited by 12 · see citing works
“CF male mice also exhibited enlarged seminal vesicles in older mice [61].”
Proteomics analysis of the bio-functions of Dregea sinensis stems provides insights regarding milk-clotting enzyme
Zhao, Q., Zhao, C., Shi, Y., Wei, G., Yang, K., Wang, X., & Huang, A. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110340 · Cited by 13 · see citing works
Initial growth response of Moringa oleifera seedlings to different soil amendments
William, J. A., Ochire-Boadu, K., & Baatuuwie, N. B. (2012) · African Journal of Agricultural Research · doi:10.5897/ajar11.2306 · Cited by 13 · see citing works
“Moringa has a great potential to become one of the most economically important crops for the tropics and subtropics considering its use in many fields as a medicine (Peixoto et al., 2011), food (Pontual et al., 2012) and fodder plant.”
Background
Enzymes in Food Processing
Fernandes, P. C. D. B., & Carvalho, F. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802392-1.00008-3 · Cited by 13 · see citing works
Production of highly active fungal milk-clotting enzyme by solid-state fermentation
Chinmayee, C. V., Vidya, C., Rani, A., & Singh, S. A. (2019) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2019.1630647 · Cited by 12 · see citing works
“Any pH greater than 8 has been found to affect casein structure and, in turn, affects the clotting time.([28,29]) This could be erroneously interpreted as effect of pH on enzyme activity.”
Background
Milk-clotting properties on bovine caseins of a novel cysteine peptidase from germinated Moringa oleifera seeds
Wang, X., Zhao, Q., Li, H., Shi, Y., Fan, J., Chen, Y., & Huang, A. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21415 · Cited by 12 · see citing works
New Coagulant Proteases for Cheesemaking from Leaves and Latex of the Spontaneous Plant Pergularia tomentosa: Biochemical Characterization of Coagulants and Sensorial Evaluation of Cheese
Leulmi, I., Zidoune, M. N., Hafid, K., Djeghim, F., Bourekoua, H., Dziki, D., & Różyło, R. (2023) · Foods · doi:10.3390/foods12132467 · Cited by 12 · see citing works
“Other clotting plant enzymes yielded similar results [20,44].”
Result
Production of a Milk-Clotting Enzyme by Glutinous Rice Fermentation and Partial Characterization of the Enzyme: Production and Characterization of a Milk-Clotting Enzyme
Zhao, X., Wang, J., Zheng, Z., Zhao, A., & Yang, Z. (2015) · Journal of Food Biochemistry · doi:10.1111/jfbc.12108 · Cited by 11 · see citing works
Milk clotting enzymes from marine resources and their role in cheese-making: A mini review
Arbita, A. A., & Zhao, J. J. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2220030 · Cited by 11 · see citing works
“Caseinolytic and milk-clotting activities (CA and MCA) measure the ability of a protease to hydrolyze casein and clot milk respectively and, thus, are closely linked to its potential for application in cheese making (Jacob, Jaros, and Rohm 2011; Pontual et al. 2012).”
Clotting Activities of Partially Purified Extracts of Moringa oleifera L. on Dromedary Camel Milk
Terefe, M. A., Kebede, A., & Kebede, M. (2018) · National Academic Digital Repository of Ethiopia · doi:10.20372/eajs.v11i2.407 · Cited by 11 · see citing works
INFLUENCE OF DREGEA SINENSIS HEMSL. PROTEASE ON THE QUALITY OF MOZZARELLA CHEESE FROM BUFFALO MILK
Wang, H., Wang, Y., & Huang, A. (2017) · Emirates Journal of Food and Agriculture · doi:10.9755/ejfa.2016-09-1227 · Cited by 10 · see citing works
“M. oleifera flowers contain caseinolytic and milk-clotting activities, which is a new source of plane proteases with potential use for cheese production, since it promotes extensive hydrolysis of κ-casein and low degradation of αs- and β-caseins (Pontual et al., 2012).”
Background
Nutritional Values, Anti-Nutritional Factors and Molar Ratio of Minerals to Anti-Nutrients of Plant-Based Yoghurt from Bambaranut, Soybean and Moringaoleifera Seed Milks
Ani, E., Igbabul, B. D., Ikya, J. K., & Amove, J. (2019) · Research Journal of Food and Nutrition · doi:10.22259/2637-5583.0304004 · Cited by 9 · see citing works
Influence of Soil Type, Sowing Date and Diluted Seawater Irrigation on Seed Germination, Vegetation and Chemical Constituents of Moringa oleifera, Lam.
Hegazi, M. A. (2015) · Journal of Agricultural Science · doi:10.5539/jas.v7n3p138 · Cited by 8 · see citing works
“…tree is particularly renowned for its great versatility, as its uses include being a food source for humans and animals alike, coagulant for water purification, remedy for numerous ailments as well as a source for biofuel production (Anwar et al., 2007; Peixoto et al., 2011; Pontual et al., 2012).”
Background
Antimicrobial activity of polypyrrole nanoparticles and aqueous extract ofMoringa oleiferaagainstStaphylococcusspp. carriers of multi-drug efflux system genes isolated from dairy farms
da Silva, J. G., de Barros, M., Santos, N. D. D. L., Paiva, P. M. G., Napoleão, T. H., de Sena, M. J., da Costa, M. M., de Oliveira, H. P., Moreira, M. A. S., & Mota, R. A. (2020) · Journal of Dairy Research · doi:10.1017/s0022029920000874 · Cited by 8 · see citing works
Self-citation
Optimization of Crude Papaya (Carica papaya) Protease in Soft-Unripened Cheese Preparation
Maskey, B., & Shrestha, N. K. (2020) · Journal of Food Science and Technology Nepal · doi:10.3126/jfstn.v12i12.30139 · Cited by 8 · see citing works
Production, characterization, and application of a new chymotrypsin-like protease from Pycnoporus sanguineus
Ferreira, A. N., da Silva, A. T., Nascimento, J., de Souza, C. B., Silva, M. D. C., Grillo, L. A. M., da Luz, J. M. R., & Pereira, H. J. V. (2023) · Biocatalysis and Biotransformation · doi:10.1080/10242422.2023.2196362 · Cited by 9 · see citing works
“Milk is composed of casein micelles, which are colloidal complexes of phosphoproteins formed by hydrophobic a s1-, a s2-, and b -caseins and stabilized in the solution by the k-casein hydrophilic domain (Pontual et al. 2012).”
Background
The Physicochemical Characteristics and Protein Profile of Cottage Cheese Produced by Using Crude Bromelain Enzyme Extracted from Ananas Comosus
Komansilan, S., Rosyidi, D., Radiati, L. E., Purwadi, P., & Evanuarini, H. (2021) · Current Research in Nutrition and Food Science Journal · doi:10.12944/crnfsj.9.2.21 · Cited by 7 · see citing works
Plant extracts as enzymes
Verma, V., Singhal, G. S., Joshi, S., Choudhary, M., & Srivastava, N. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822475-5.00009-0 · Cited by 7 · see citing works
Identification, structure, and caseinolytic properties of milk-clotting proteases from Moringa oleifera flowers
Zhao, Q., Huang, A., Wu, G., Guo, Q., Li, M., & Wang, X. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111598 · Cited by 7 · see citing works
Characterization of a Milk-clotting Enzyme from Hericium erinaceum and Its Proteolytic Action on Bovine Caseins
Sato, K., Goto, K., Suzuki, A., Miura, T., Endo, M., Nakamura, K. T., & Tanimoto, M. (2018) · Food Science and Technology Research · doi:10.3136/fstr.24.669 · Cited by 7 · see citing works
“The specificity of caseinolytic activity from Moringa oleifera flowers indicated that hydrolysis of κ-casein started after 30 min of incubation, while αs- and β-caseins were degraded after 60 min (Pontual et al., 2012).”
Background
Proteases from Calotropis gigantea stem, leaf and calli as milk coagulant source
Rajagopalan, A., Soundararajan, M., & Sukumaran, B. O. (2018) · Turkish Journal of Biochemistry · doi:10.1515/tjb-2017-0268 · Cited by 6 · see citing works
Purification and Characterization of Milk Clotting Enzyme from Edible Mushroom (Pleurotus florida)
(2021) · Letters in Applied NanoBioScience · doi:10.33263/lianbs112.33623373 · Cited by 7 · see citing works
“However, low saturation of ammonium sulfate is superior to high concentrations usually used to purify MCE from different plants [29, 30].”
Background
METALLOPROTEASE FROM THE CULTURAL LIQUID OF Pleurotus osreatus
Sakovich, V. V. (2019) · Biotechnologia Acta · doi:10.15407/biotech12.06.035 · Cited by 6 · see citing works
Moringa oleifera Lam. Proteins: Properties and Food Applications
D’Auria, G., Nitride, C., & Ferranti, P. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-823960-5.00005-6 · Cited by 6 · see citing works
Characterization of Ficus benjamina and Artocarpus heterophyllus Proteases as Potential Rennet Alternatives
Sukmana, A. B. A., Widyaningrum, I., Lani, R. K., & Kasmiyati, S. (2020) · Biosaintifika Journal of Biology & Biology Education · doi:10.15294/biosaintifika.v12i2.23516 · Cited by 5 · see citing works
“Milk coagulation screening assay Milk coagulation potential for the screening was conducted by mixing 20 μl crude enzyme extract with 5 ml 10% skim milk containing 10 mM CaCl2 in phosphate buffer pH 7.0 (Pontual et al., 2012).”
MethodInfluential
Proteomics-guided isolation of a novel serine protease with milk-clotting activity from tamarillo (Solanum betaceum Cav.)
Zhang, T., Yang, Z., Zhang, Y., Yi, L., Duan, F., Zhao, Q., Gu, Y., & Wang, S. (2024) · Food Chemistry · doi:10.1016/j.foodchem.2024.141956 · Cited by 5 · see citing works
Development of a cereal-based product using residual Moringa oleifera Lam. seed powder biomass and pseudo-plastic behavior of the dough mixtures
Paz, R., Landázuri, A. C., & Vernaza, M. G. (2020) · Nutrition & Food Science · doi:10.1108/nfs-05-2020-0161 · Cited by 4 · see citing works
Optimisation of soft camel cheese production coagulated with green carob extract using response surface methodology: Yield, physicochemical, microbial, sensorial, and rheological properties
Omrani, A., Sboui, A., Hannachi, H., Hamouda, M., Dbara, M., Hammadi, M., Khorchani, T., & Maqsood, S. (2024) · International Journal of Dairy Technology · doi:10.1111/1471-0307.13118 · Cited by 4 · see citing works
Optimizing glutamate production from microalgae extracts sourced from the Caspian Sea Basin: A cost-effective and sustainable approach with potential applications
Mirsalami, S. M., & Mirsalami, M. (2024) · Food Chemistry Advances · doi:10.1016/j.focha.2024.100742 · Cited by 4 · see citing works
Initial growth of Moringa oleifera Lam. under different planting densities in autumn/winter in south Brazil
Poliana, F. D. C., Borsoi, A., Edmar, S. D. V., & Piano, J. T. (2015) · African Journal of Agricultural Research · doi:10.5897/ajar2013.7549 · Cited by 4 · see citing works
Improvement of camel milk-clotting: Usefulness of crude extract from green pods of carob (Ceratonia siliqua. L) as a substitute for commercial rennet.
Omrani, A., Sboui, A., Mars, W., Hamouda, M., Dbara, M., Hammadi, M., & Khorchani, T. (2023) · Cellular and Molecular Biology · doi:10.14715/cmb/2023.69.13.14 · Cited by 1 · see citing works
“…Liang et al. (34) showed an increase in SA in pineapple peels as the temperature increased, with a maximum value of 55 °C. Similar results were obtained with Moringa oleifera flower and Opuntia ficus-indica fruit extracts with optimal clotting activity at 50 °C and 55 °C, respectively (35,36).”
Result
Yoghurt fortification with Moringa oleifera: nutritional and production aspects
Adepoju, F. O. (2023) · Mljekarstvo/Mljekarstvo.com · doi:10.15567/mljekarstvo.2024.0101 · Cited by 3 · see citing works
“Leaf extracts of M. oleifera have been shown to be useful in diabetes treatment, with the ability to lower blood sugar levels and improve antioxidant status (Olurishe et al., 2016; Pontual et al., 2012).”
Characteristics of Coagulants from Moringa Seed Extract (Moringa oleifera) with Microwave-Assisted Extraction (MAE) Method
Putri, G. M., Manab, A., & Awwaly, K. U. A. (2022) · Asian Food Science Journal · doi:10.9734/afsj/2022/v21i930457 · Cited by 2 · see citing works
“The MCA was determined using [23] 10% skimmed milk (w/v).”
Method
From Beer to Cheese: Characterization of Caseinolytic and Milk-Clotting Activities of Proteases Derived from Brewer’s Spent Grain (BSG)
Villegas, M. M., SILVA, J. N. F., Tito, F. R., Tonón, C. V., Muñoz, F. F., Pepe, A., & Guevara, M. G. (2024) · Foods · doi:10.3390/foods13223658 · Cited by 2 · see citing works
“BSG’s endopeptidases activity on bovine α -, β -, and k-casein subunits was determined according to the method described by Pontual [32].”
Plant Proteases in Food Processing
Shah, M. A., & Mir, S. A. (2018) · Reference series in phytochemistry · doi:10.1007/978-3-319-54528-8_68-1 · Cited by 2 · see citing works
Chromatographic purification of the enzyme preparation from the cultural liquid of Pleurotus ostreatus
Sakovich, V. V., Hrusha, A. M., Revenko, V. V., & Zhernossekov, D. D. (2019) · Proceedings of the National Academy of Sciences of Belarus Biological Series · doi:10.29235/1029-8940-2019-64-4-467-471 · Cited by 2 · see citing works
RESPONSE OF MORINGA OLEIFERA TO ORGANIC BIOFERTILIZERS AND MINERAL NPK FERTILIZATIONS
Abdou, M. A. H., El-Sayed, A., Taha, G., & Mosaad, S. (2016) · Scientific Journal of Flowers and Ornamental Plants /Scientific Journal of Flowers and Ornamental Plants · doi:10.21608/sjfop.2016.5120 · Cited by 2 · see citing works
“Moringa has a great potential to become one of the most economically important crops for the tropics and subtropics considering its use in many fields as a medicine (Peixoto et al., 2011), food (Pontual et al., 2012) and fodder plant.”
BackgroundResult
Effect of Moringa oleifera Leaves on Human Blood Coagulation Process
Sander, Y., Ariesanti, Y., & Roeslan, M. O. (2021) · IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce) · doi:10.1109/inhence52833.2021.9537224 · Cited by 2 · see citing works
Use of Proteolytic Activity of Ficus carica in Milk Coagulation
Priyashantha, H., Ranadheera, C. S., Senadheera, T. R. L., Hettiarachchi, H. T. M., Jayarathna, S., & Vidanarachchi, J. K. (2023) · Springer eBooks · doi:10.1007/978-3-031-16493-4_33 · Cited by 2 · see citing works
Screening and isolation of milk-clotting enzymes from seaweed
Arbita, A. A., Paul, N. A., Cox, J. M., & Zhao, J. J. (2023) · Journal of Applied Phycology · doi:10.1007/s10811-023-02975-4 · Cited by 2 · see citing works
“Several studies conducted only single stage of ammonium sulphate precipitation, e.g., for enzymes extracted from Moringa oleifera and tamarillo fruit (Pontual et al. 2012; Li et al. 2018a).”
EFFECT OF BIO-FERTILIZATION AND GROWING MEDIA ON THE CHEMICAL COMPOSITION OF MORINGA OLEIFERA TREES
Sarhan, A. Z., Arfa, A., & Gonaw, H. (2017) · Scientific Journal of Flowers and Ornamental Plants /Scientific Journal of Flowers and Ornamental Plants · doi:10.21608/sjfop.2017.11322 · Cited by 2 · see citing works
“It is also a fast growing plant (Amaglo et al., 2007; Peixoto et al., 2011; Pontual et al., 2012).”
Background
CHARACTERIZATION OF MILK CLOTTING PROPERTIES OF ADULT CATTLE RENNET MODIFIED WITH Moringa oleifera SEEDS AND ITS SUITABILITY AS CALF RENNET ALTERNATIVE IN CHEESE MAKING
Ismail, H. A. (2019) · Zagazig Journal of Agricultural Research · doi:10.21608/zjar.2019.40962 · Cited by 2 · see citing works
“It has been concluded that Moringa oleifera plant is an exciting source of milk clotting enzymes such as aspartic enzyme in different parts (Pontual et al., 2012).”
BackgroundResultInfluential
Chymosin, Pepsins, and Other Aspartyl Proteinases: Structures, Functions, Catalytic Mechanism and Milk-Clotting Properties
Uniacke‐Lowe, T., & Fox, P. F. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-15956-5.00027-0 · Cited by 1 · see citing works
Influence of sowing date and organic fertilizers on productivity of Moringa oleifera plant under Aswan governorate conditions, Egypt
Mahmoud, H., Hassan, E., & Aboelfetoh, M. (2021) · Archives of Agriculture Sciences Journal/Archives of Agriculture Sciences Journal · doi:10.21608/aasj.2021.179861 · Cited by 1 · see citing works
“Moringa has a great potential to become one of the most economically important crops for the tropics and subtropics considering its use in many fields as a medicine (Peixoto et al., 2011), food (Pontual et al., 2012) and fodder plant.”
Background
Preparação de flores de Moringa oleifera causa mortalidade de Nasutitermes corniger (Isoptera: Termitidae) por bloquear a atividade de tripsina e inibir o crescimento da microbiota intestinal dos insetos
de Almeida, W. A., de Moura, M. C., Lima, T. D. A., de Albuquerque, L. P., Napoleão, T. H., Paiva, P. M. G., & Pontual, E. V. (2016) · Revista Arrudea - A revista do Jardim Botânico do Recife · doi:10.55513/arrudea0010 · Cited by 1 · see citing works
Self-citation
Optimization of caseinolytic and coagulating activities of Solanum tuberosum rennets for cheese making
Tito, F. R., Pepe, A., Tonón, C. V., Daleo, G. R., & Guevara, M. G. (2023) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.12780 · Cited by 1 · see citing works
SHOBHANJANA (MORINGA OLEIFERA LAM.) FLOWER: A PHARMACOLOGICAL REVIEW
Khobragade, V., Singh, R. K., Tawalare, K. A., Joshi, P. K., & Chauhan, N. S. (2024) · International Ayurvedic Medical Journal · doi:10.46607/iamj13p8062024 · Cited by 1 · see citing works
Ethnomedicinal Uses of the Flora as an Alternative During the Treatment of SARS-CoV-2 in the Mayan Communities of the Yucatan Peninsula, Mexico
Ríos-Oviedo, Á., Cetzal-Ix, W. R., López-Castilla, H. M. J., Basu, S. K., Tamayo‐Cen, I., & la Torre-Espinosa, Z. Y. D. (2025) · Journal of Herbs Spices & Medicinal Plants · doi:10.1080/10496475.2025.2498921 · Cited by 1 · see citing works
“[8] These compounds can inhibit virus proliferation by blocking essential enzymes or viral entry into host cells.”
PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS
Dash, P., & Ghosh, G. (2015) · International Journal of Pharmacy and Pharmaceutical Sciences
Effect of high isostatic pressure processing on milk-clotting enzymes
Júnior, B. R. D. C. L. (2017) · doi:10.47749/t/unicamp.2017.980662
Effect of pH on Clotting Properties from Moringa Oleifera Seeds with Focused Microwave-Assisted Soxhlet Extraction Method as Milk Coagulant
Aprilianty, A., Nuraini, R., Pebriani, N., Apriani, G., Manab, A., Umam, K., Mustakim, & Andriani, R. D. (2023) · Advances in biological sciences research/Advances in Biological Sciences Research · doi:10.2991/978-94-6463-116-6_49
Production of Glutamate by Pseudomonas Putida Using Microalgae Extract of Caspian Sea Basin as SubstratePreprint
Mirsalami, S. M., & Mirsalami, M. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4635440
Moringa oleifera
Abubakar, A. S., & Ismail, B. B. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13769-3.00010-8
Exploiting pitaya cladodes for protease Extraction: Immobilization on nanomaterials and its role in antioxidant production
Alves, A. N., Santos, I. A., Nascimento, P. A., Porfírio, M. C. P., Boas, E. V. D. B. V., Fontan, R. D. C. I., & Bonomo, R. C. F. (2025) · Separation and Purification Technology · doi:10.1016/j.seppur.2025.134994
An Overview of West African Traditional Soft Cheese: Processing, Safety, and Quality Characteristics
Tossou, M. W., Achaglinkame, M. A., Dari, L., Sossa-Vihotogbé, C., Bodor, Z., & Mörlein, D. (2025) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.70301
Edible Tree Flowers as Emerging Functional Foods: A Comprehensive Review of Nutritional Composition, Bioactive Phytochemicals, Therapeutic Potential, and Safety Profile
Hiremath, V. M., Yadav, R., Tyagi, S. K., Pooja, A., Lakshmi Durga, M., Rajvardhan, P., Tomar, M., J, S. A., Urhe, S. B., Kawar, P. G., et al. (2026) · Food Nutrition · doi:10.1016/j.fnutr.2026.100097

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Authors: Pontual, E. V., Carvalho, B. E. A., Bezerra, R. S., Coelho, L. C. B. B., Napoleão, T. H., & Paiva, P. M. G.
Affiliations: Universidade Federal de Pernambuco (BR)
Corresponding author: Paiva, Patrícia M.G.
ORCID: Pontual, Emmanuel V.
Coelho, Luana C.B.B.
Napoleão, Thiago H.
Paiva, Patrícia M.G.
Venue: Food Chemistry
Publisher: Elsevier BV
Published: 2012-12
Volume: 135
Issue: 3
Pages: 1848-1854
ISSN: 0308-8146 (ISSN-L)
DOI: 10.1016/j.foodchem.2012.06.087
OpenAlex: W2081770308
Semantic Scholar: 0d6e9ca8a1e33e42b05006250704381fa3c9e60f
Cited by: 97
References: 53
Citations by year: 2026: 1 · 2025: 4 · 2024: 6 · 2023: 12 · 2022: 6 · 2021: 8 · 2020: 11 · 2019: 10 · 2018: 10 · 2017: 12 · 2016: 7 · 2015: 5 · 2014: 1 · 2013: 3 · 2012: 1
FWCI: 3.63
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 237 d)
Locations: Food Chemistry (publishedVersion)
PubMed (publishedVersion)
Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Moringa oleifera research and applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords: Moringa oleifera, caseinolytic activity, milk-clotting activity, plant proteases, aspartic protease, κ-casein hydrolysis, protease inhibitors, pH and temperature stability
MeSH terms: Animals, Caseins, Cattle, Hydrogen-Ion Concentration, Hydrolysis, Milk, Plant Extracts, Moringa oleifera, Flowers

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Caseinolytic and milk-clotting activities from Moringa oleifera flowers

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