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Cited by 71Bee Products Chemical AnalysisEssential Oils and Antimicrobial ActivityPhytochemistry and Biological Activities

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Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum

Yang, S.Z., Peng, L.T., Su, X.J., Chen, F., Cheng, Y.J., Fan, G., Pan, S.Y.·2011·Food Chemistry
DOI 10.1016/j.foodchem.2010.12.011
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Abstract

The present study was aimed at identification of antifungal components against Penicillium italicum from Chinese propolis with bioassay-guided fractionation technique. Propolis ethanolic extract (PEE) was separated and purified by liquid–liquid extraction and thin layer chromatography (TLC) and the most active band was subjected to HPLC–MS/MS to identify the antifungal compounds. The results showed PEE and its fractions had strong antifungal activity against P. italicum. Among the fractions of PEE partitioned by petroleum ether, ethyl acetate, n-butanol and water, ethyl acetate fraction (E-Fr) exhibited the most effective activity against P. italicum. Further bioautographic TLC assay showed Band I, with Rf value of 0.70, had an inhibitive zone, which showed the strongest antifungal activity and completely inhibited the growth of P.italicum at 200mg/L. Bioactive components found in Band I were further identified as pinobanksin, pinocembrine, chrysin and galangin. This study exhibited Chinese propolis and its main flavonoids was potential natural alternatives for the control of citrus blue mould caused by P.italicum.

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Metadata

Authors
Yang, S. Z., Peng, L. T., Su, X. J., Chen, F., Cheng, Y. J., Fan, G., & Pan, S. Y.
Affiliations
  • Huazhong Agricultural University (CN)
  • Hunan Agricultural University (CN)
  • Clemson University (US)
Corresponding author
  • Yang, S.Z.
  • Pan, S.Y.
ORCID
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2011-07
Volume
127
Issue
1
Pages
210-215
ISSN
0308-8146 (ISSN-L)
DOI
10.1016/j.foodchem.2010.12.011
OpenAlex
W2065022898
Cited by
71
References
0
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 4 · 2020: 8 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 5 · 2014: 3 · 2013: 4 · 2012: 6
FWCI
4.17
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
Food Chemistry (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
Keywords
propolis, Penicillium italicum, antifungal activity, bioassay-guided fractionation, flavonoids, pinobanksin, chrysin, galangin, natural fungicides, ethanolic propolis extract

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CoChat. (2026). Citation check: Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum. https://app.cochat.ai/papers/10.1016/j.foodchem.2010.12.011
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Yang, S. Z., Peng, L. T., Su, X. J., Chen, F., Cheng, Y. J., Fan, G., & Pan, S. Y. (2011). Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum. Food Chemistry. https://doi.org/10.1016/j.foodchem.2010.12.011