The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Comparison of the milk-clotting properties of three plant extracts
Abstract
Several proteases from plant sources have been proposed as milk coagulants, however, limited research has been done on their milk-clotting properties. The effect of temperature on the milk-clotting activity of kiwi fruit, melon and ginger extracts was evaluated, as well as the effects of the different extracts on curd properties. Melon extracts showed high milk-clotting activity over a broad temperature range (45-75 °C) while kiwi fruit and ginger extracts showed high activity over a narrower temperature range, with a maximum at 40 and 63 °C, respectively. Curds produced using kiwi extracts had textural properties comparable with those obtained using commercial rennet, while melon extracts produced a fragile gel and low curd yield. The milk-clotting behavior of the three plant extracts was related to the protease specificity present in these extracts. The kiwi proteases displayed chymosin-like properties and thus hold the best potential for use as a milk coagulant in cheese production.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Mazorra-Manzano et al. [58] compared proteases extracted from ginger (Zingiber officinale) named zingibaine, melon (Cucumis melo) named cucumisin, and kiwifruit (Actinidia deliciosa) named actinidin, revealing that actinidin has the highest MCA/PA ratio compared to other vegetable coagulants.”
From Plant Milk-Clotting Enzymes for Cheesemaking (2022) · cites this paper as [58]
In the citing paper
“On the contrary, curds obtained using melon extracts had different textural properties as reflected by the low values of hardness, cohesiveness, chewiness, and springiness, possibly due to the higher PA and the lower MCA/PA ratio [58].”
From Plant Milk-Clotting Enzymes for Cheesemaking (2022) · cites this paper as [58]
Citations
Cites
CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR
Kunitz, M. (1947) · The Journal of General Physiology · doi:10.1085/jgp.30.4.291 · Cited by 2,459
Recent Advances in Milk Clotting Enzymes
JACOB, M., JAROS, D., & ROHM, H. (2011) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2010.00633.x · Cited by 295
Cheesemaking with vegetable coagulants—the use of Cynara L. for the production of ovine milk cheeses
Roseiro, L. B., Barbosa, M., Ames, J. M., & Wilbey, R. A. (2003) · International Journal of Dairy Technology · doi:10.1046/j.1471-0307.2003.00080.x · Cited by 200
Native and Biotechnologically Engineered Plant Proteases with Industrial Applications
Feijoo-Siota, L., & Villa, T. G. (2010) · Food and Bioprocess Technology · doi:10.1007/s11947-010-0431-4 · Cited by 171
[30] Milk-clotting enzyme from Mucor pusillus var. Lindt
Arima, Κ., Yu, J., & Iwasaki, S. (1970) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(70)19033-1 · Cited by 170
Ver�ssimo, P., Esteves, C., Faro, C., & Pires, E. (1995) · Biotechnology Letters · doi:10.1007/bf00129389 · Cited by 148
Egito, A. S., Girardet, J.-M., Laguna, L. E., Poirson, C., Mollé, D., Miclo, L., Humbert, G., & Gaillard, J.-L. (2007) · International Dairy Journal · doi:10.1016/j.idairyj.2006.09.012 · Cited by 138
GINGER RHIZOME: A NEW SOURCE OF PROTEOLYTIC ENZYME
Thompson, E. H., Wolf, I. D., & Allen, C. E. (1973) · Journal of Food Science · doi:10.1111/j.1365-2621.1973.tb02836.x · Cited by 112
Lobato‐Calleros, C., Reyes‐Hernández, J., Beristain, C. I., HORNELAS‐URIBE, Y., Sánchez-García, J. E., & Vernon‐Carter, E. J. (2006) · Food Research International · doi:10.1016/j.foodres.2006.10.011 · Cited by 106
Isolation and Characterization of a Proteinase from the Sarcocarp of Melon Fruit
Kaneda, M., & Tominaga, N. (1975) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a131026 · Cited by 97
Research works printed but not linked: 1
- Hashinaga, 1986, Activity and distribution of proteases in kiwifruit during ripening, Bulletin of the Faculty of Agriculture Kagoshima University, 36, 65
Cited by
Sorted by citations
Plant proteases for bioactive peptides release: A review
Mazorra-Manzano, M. A., Ramírez‐Suárez, J. C., & Yada, R. Y. (2017) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2017.1308312 · Cited by 202
Self-citationAmira, A. B., Besbes, S., Attia, H., & Blecker, C. S. (2017) · International Journal of Food Properties · doi:10.1080/10942912.2017.1289959 · Cited by 135
Production of Plant Proteases and New Biotechnological Applications: An Updated Review
Troncoso, F. D., Sánchez, D. A., & Ferreira, M. L. (2022) · ChemistryOpen · doi:10.1002/open.202200017 · Cited by 127
Plant Milk-Clotting Enzymes for Cheesemaking
Nicosia, F. D., Puglisi, I., Pino, A., Caggia, C., & Randazzo, C. L. (2022) · Foods · doi:10.3390/foods11060871 · Cited by 95
“On the contrary, curds obtained using melon extracts had different textural properties as reflected by the low values of hardness, cohesiveness, chewiness, and springiness, possibly due to the higher PA and the lower MCA/PA ratio [58].”
BackgroundTrends in kiwifruit and byproducts valorization
Sanz, V., López‐Hortas, L., Torres, M. D., & Domı́nguez, H. (2020) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2020.11.010 · Cited by 81
Metadata
- Authors
- Mazorra-Manzano, M. A., Perea-Gutiérrez, T. C., Lugo-Sánchez, M. E., Ramirez-Suarez, J. C., Torres-Llanez, M. J., González-Córdova, A. F., & Vallejo-Cordoba, B.
- Affiliations
- Centro de Investigación en Alimentación y Desarrollo (MX)
- Corresponding author
- Mazorra-Manzano, Miguel A.
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 141
- Issue
- 3
- Pages
- 1902-1907
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2060044435
- Cited by
- 117
- References
- 44
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 14 · 2023: 15 · 2022: 12 · 2021: 9 · 2020: 16 · 2019: 13 · 2018: 10 · 2017: 8 · 2016: 5 · 2015: 2 · 2014: 1
- FWCI
- 3.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Science and Technology National Council (CONACyT)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pineapple and bromelain studies
- Keywords
- plant proteases, milk coagulants, ginger extract, milk-clotting activity, temperature dependence, cheese production
- MeSH terms
- Animals, Cattle, Cheese, Food Additives, Hydrogen-Ion Concentration, Milk, Plant Extracts, Temperature, Cucurbitaceae, Zingiber officinale, Actinidia
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review