The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?
Abstract
In this study Ocimum basilicum L. (OB) and Ocimum tenuiflorum L. (OT) in vitro culture standardisation for increasing eugenol distribution, in comparison to their respective field grown parts was carried out. Eugenol was quantified using an optimised HPLC method and its relation with the total phenolic content (TPC) was measured. In vitro grown leaves and somatic embryos, of both OB and OT were found to contain similar quantities of eugenol (85μg/g approximately), higher than OB and OT field-grown leaves (30.2μg/g and 25.1μg/g respectively). It was also determined that in vitro grown leaves were richer in TPC than the field-grown intact organs. Results demonstrated the prominence of in vitro cultures for eugenol extraction. This study underlines that important food flavouring metabolites (e.g. vanillin, vanillic acids) might be produced, via the eugenol pathway, in Ocimum species that may be a good potential source of eugenol.
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“A significant number of studies have been devoted to describing Ocimum essential oils [21,29,30], but relatively few studies have been devoted to Ocimum leaves as there is a lack of a comprehensive and rapid fingerprinting approach [16,31,32].”
From Comprehensive Metabolomic Fingerprinting Combined with Chemometrics Identifies Species- and Variety-Specific Variation of Medicinal Herbs: An Ocimum Study (2023) · cites this paper as [32]
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Cites
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Current approaches toward production of secondary plant metabolites
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A phytopharmacological overview on Ocimum species with special emphasis on Ocimum sanctum
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- Anamika, 2013, Evaluation of ethanolic extract of Ocimum tenuiflorum for antiepileptic activity, Indian Journal of Pharmacology, 45, S235
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Gurav, T. P., Dholakia, B. B., & Giri, A. P. (2022) · Phytochemistry Reviews · doi:10.1007/s11101-021-09767-z · Cited by 87
Rai, A. K., Khan, S., Kumar, A., Dubey, B. K., Lal, R. K., Tiwari, A., Trivedi, P. K., Elliott, C. T., & Ch, R. (2023) · Metabolites · doi:10.3390/metabo13010122 · Cited by 33
“A significant number of studies have been devoted to describing Ocimum essential oils [21,29,30], but relatively few studies have been devoted to Ocimum leaves as there is a lack of a comprehensive and rapid fingerprinting approach [16,31,32].”
Background
Metadata
- Authors
- Bhuvaneshwari, K., Gokulanathan, A., Jayanthi, M., Govindasamy, V., Milella, L., Lee, S., Yang, D. C., & Girija, S.
- Affiliations
- Bharathiar University (IN)
- University of Basilicata (IT)
- Kyung Hee University (KR)
- Corresponding author
- Yang, Deok Chun
- Girija, Shanmugam
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2016-03
- Volume
- 194
- Pages
- 55-60
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W1130223258
- Cited by
- 30
- References
- 29
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 4
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutional Research Information System (University of Basilicata) (submittedVersion)
- Funders
- Ministry of Knowledge Economy
- NIPA
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- Ocimum basilicum, Ocimum sanctum, in vitro culture, eugenol, secondary metabolites, total phenolic content, somatic embryos, vanillin, vanillic acid, HPLC determination
- MeSH terms
- Chromatography, High Pressure Liquid, Eugenol, Plant Leaves, Ocimum basilicum, In Vitro Techniques
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