Cited by 30Plant tissue culture and regenerationBiochemical and biochemical processesPlant biochemistry and biosynthesis

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

Abstract

In this study Ocimum basilicum L. (OB) and Ocimum tenuiflorum L. (OT) in vitro culture standardisation for increasing eugenol distribution, in comparison to their respective field grown parts was carried out. Eugenol was quantified using an optimised HPLC method and its relation with the total phenolic content (TPC) was measured. In vitro grown leaves and somatic embryos, of both OB and OT were found to contain similar quantities of eugenol (85μg/g approximately), higher than OB and OT field-grown leaves (30.2μg/g and 25.1μg/g respectively). It was also determined that in vitro grown leaves were richer in TPC than the field-grown intact organs. Results demonstrated the prominence of in vitro cultures for eugenol extraction. This study underlines that important food flavouring metabolites (e.g. vanillin, vanillic acids) might be produced, via the eugenol pathway, in Ocimum species that may be a good potential source of eugenol.

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Crossref · 10.1016/j.foodchem.2015.07.136

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  1. Anamika, 2013, Evaluation of ethanolic extract of Ocimum tenuiflorum for antiepileptic activity, Indian Journal of Pharmacology, 45, S235

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Metadata

Authors
Bhuvaneshwari, K., Gokulanathan, A., Jayanthi, M., Govindasamy, V., Milella, L., Lee, S., Yang, D. C., & Girija, S.
Affiliations
  • Bharathiar University (IN)
  • University of Basilicata (IT)
  • Kyung Hee University (KR)
Corresponding author
  • Yang, Deok Chun
  • Girija, Shanmugam
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2016-03
Volume
194
Pages
55-60
ISSN
0308-8146 (ISSN-L)
OpenAlex
W1130223258
Cited by
30
References
29
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 4
FWCI
1.16
Type
journal article
Language
en
Open access
closed
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • CINECA IRIS Institutional Research Information System (University of Basilicata) (submittedVersion)
Funders
  • Ministry of Knowledge Economy
  • NIPA
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Ocimum basilicum, Ocimum sanctum, in vitro culture, eugenol, secondary metabolites, total phenolic content, somatic embryos, vanillin, vanillic acid, HPLC determination
MeSH terms
Chromatography, High Pressure Liquid, Eugenol, Plant Leaves, Ocimum basilicum, In Vitro Techniques

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