Cited by 224Proteins in Food SystemsProtein Hydrolysis and Bioactive PeptidesProtein Structure and Dynamics

The aggregation, structures and emulsifying properties of soybean protein isolate induced by ultrasound and acid

Huang, Liurong, Ding, Xiaona, Li, Yunliang, Ma, Haile·2019·Food Chemistry

Abstract

The effects of ultrasound and acid on the aggregation, structures and emulsifying properties of soybean protein isolate (SPI) were investigated. Results of solubility showed that ultrasonic treatments at 0.001 M HCl increased the content of soluble SPI. The particle size of soluble aggregates subjected to ultrasonication and acid was initially decreased and then increased with increasing ultrasonic time. Secondary structure analysis, by circular dichroism, indicated lower a-helix and higher random coil amounts in SPI treated with short ultrasonic time, in contrast to the higher a-helix and lower random coil in SPI treated with longer time (more than 20 min). Emulsions prepared with SPI by 10 min of ultrasonication demonstrated significantly (P < 0.05) small droplet sizes and long term stability in comparison with their untreated counterparts. These results highlight that the emulsifying properties of SPI can be significantly improved by the synergistic effect of ultrasound and acid.

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Crossref · 10.1016/j.foodchem.2018.11.147

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Authors
Huang, L., Ding, X., Li, Y., & Ma, H.
Affiliations
Jiangsu University (CN)
Corresponding author
Huang, Liurong
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2019-05
Volume
279
Pages
114-119
ISSN
0308-8146 (ISSN-L)
OpenAlex
W2903645087
Cited by
224
References
37
Citations by year
2026: 34 · 2025: 37 · 2024: 45 · 2023: 34 · 2022: 25 · 2021: 23 · 2020: 19 · 2019: 7
FWCI
11.53
Type
journal article
Language
en
Open access
closed
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31701540)
  • Natural Science Foundation of Jiangsu Province (31701538, BK20170539)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
soy protein isolate, ultrasound treatment, acid treatment, protein aggregation, protein secondary structure, emulsifying properties, emulsion stability, droplet size, protein solubility, helix content
MeSH terms
Circular Dichroism, Emulsions, Hydrochloric Acid, Particle Size, Solubility, Sonication, Spectrophotometry, Soybean Proteins, Protein Aggregates

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