Cited by 133Collagen: Extraction and CharacterizationDyeing and Modifying Textile FibersProteins in Food Systems

Characterization and comparison of collagen extracted from the skin of the Nile tilapia by fermentation and chemical pretreatment

Open access
Song, Zengliu, Liu, Huanming, Chen, Liwen, Chen, Leilei, Zhou, Chunxia, Hong, Pengzhi, Deng, Chujin·2021·Food Chemistry

Abstract

Chemical pretreatment of collagen raw materials is time-consuming and environmentally hazardous. Collagen extraction after fermentation pretreatment has not been reported. We extracted and characterized acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from Nile tilapia (Oreochromis niloticus) skin following fermentation and chemical treatments and comparatively evaluated the feasibility of fermentation. Fermentation-ASC (FASC) and fermentation-PSC (FPSC) yields (4.76 and 8.14 wt%, respectively) were slightly but not significantly higher than chemical-ASC (CASC) and chemical-PSC (CPSC) yields (4.27 and 7.60 wt%, respectively). All extracts were identified as type I collagens by SDS-PAGE and retained their triple helical structure well, as confirmed through Fourier transform infrared spectroscopy. All collagen microstructures under scanning electron microscopy were multi-layered aggregates. These collagens also had similar biochemical properties (i.e. denatured between 36.5 and 37.1 °C, high soluble at pH 1-4 and at <3% [w/v] NaCl). Therefore, fermentation method is a viable alternative for pretreating collagen extraction materials.

Integrity signals

Registry

Crossref · 10.1016/j.foodchem.2020.128139

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Nile tilapia skin fermentation followed by extraction could be applied as a time-saving and environmentally friendly method for obtaining fish-based collagen with potential application in skin-care products.

    “Nile tilapia (Oreochromis niloticus) skin fermentation followed by extraction could also be applied as a time-saving and environmentally friendly method for obtaining fish-based collagen with a potential application in skin-care products [109].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Song, Z., Liu, H., Chen, L., Chen, L., Zhou, C., Hong, P., & Deng, C.
Affiliations
Guangdong Ocean University (CN)
Corresponding author
Liu, Huanming
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2021-03
Volume
340
Pages
128139
ISSN
0308-8146 (ISSN-L)
OpenAlex
W3088098342
Cited by
133
References
34
Citations by year
2026: 12 · 2025: 26 · 2024: 33 · 2023: 22 · 2022: 28 · 2021: 12
FWCI
4.03
Type
journal article
Language
en
Open access
Locations
  • Food Chemistry (acceptedVersion)
  • PubMed (publishedVersion)
Funders
  • State Oceanic Administration
  • Department of Agriculture of Guangdong Province
  • Guangdong Science and Technology Department
  • Ministry of Education of the People's Republic of China
Topics
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Physical Sciences › Engineering › Building and Construction › Dyeing and Modifying Textile Fibers
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
collagen extraction, Nile tilapia skin, Oreochromis niloticus, chemical pretreatment, acid-soluble collagen, pepsin-soluble collagen, type I collagen, triple-helical structure, collagen microstructure, collagen yield, fish skin by-products
MeSH terms
Amino Acids, Animals, Bacillus, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Pepsin A, Skin, Sodium Chloride, Solubility, Spectroscopy, Fourier Transform Infrared, Cichlids, Collagen Type I, Fish Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review