Cited by 27Meat and Animal Product QualityProteins in Food SystemsMicrobial Inactivation Methods

Modifying yellowfin tuna myofibrillar proteins under ultra-high pressure auxiliary heat treatment: Impact on the conformation, gel properties and digestive characteristics

Tan, Zhifeng, Yang, Xiaoqing, Zhao, Weiping, Ma, Jingting, Fan, Xin, Liu, Zhengqi, Dong, Xiuping·2025·Food Chemistry

Abstract

This study investigated the changes in physicochemical and functional properties of yellowfin tuna myofibrillar protein (MP) under various ultra-high pressure (UHP) auxiliary heat methods. The UHP-assisted heat treatment induced a rearrangement of the MP secondary structure, facilitating the formation of MP gel networks and resulting in higher storage modulus (G') values. Microstructure results revealed that MP gel produced with UHP auxiliary heat exhibited a more rigid network. As pressure increased, the regular aggregation of protein molecules enhanced the stability and water-binding capacity within the gel network, particularly under the two-stage UHP auxiliary heat (TUH) condition at 300 MPa. MP gel prepared under this condition exhibited a 1.95-fold increase in gel strength compared to the control group and the lowest creep strain. Furthermore, in vitro simulated digestion results indicated that TUH method significantly improved the digestive properties of MP gel, suggesting potential for the development of easily digestible MP-based gel foods.

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Crossref · 10.1016/j.foodchem.2025.143365

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  • This structural rearrangement from UHP led to higher mechanical strength and protein digestibility, attributed to UHP-induced exposure of additional enzymatic cleavage sites on protein molecules.

    “This structural rearrangement led to higher mechanical strength and protein digestibility, attributed to UHP‐induced exposure of additional enzymatic cleavage sites on protein molecules”

    From Designing Protein Gels for Nutrient Delivery: From Formulation to Digestion (2026) · cites this paper as Z. Tan, Yang, et al. 2025

  • UHP treatment can modulate the secondary structure of proteins, promoting molecular chain unfolding and ordered protein aggregation.

    “UHP treatment can modulate the secondary structure of proteins, promoting molecular chain unfolding and ordered protein aggregation.”

    From Designing Protein Gels for Nutrient Delivery: From Formulation to Digestion (2026) · cites this paper as Z. Tan, Yang, et al. 2025

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Authors
Tan, Z., Yang, X., Zhao, W., Ma, J., Fan, X., Liu, Z., & Dong, X.
Affiliations
  • Dalian Polytechnic University (CN)
  • Shenzhen University (CN)
Corresponding author
Dong, Xiuping
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2025-05
Volume
475
Pages
143365
ISSN
0308-8146 (ISSN-L)
OpenAlex
W4407316632
Cited by
27
References
52
Citations by year
2026: 15 · 2025: 12
FWCI
16.65
Type
journal article
Language
en
Open access
closed
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Key Research and Development Program of China (2023YFD2100200)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Keywords
yellowfin tuna, myofibrillar proteins, ultra-high pressure processing, protein secondary structure, gel properties, storage modulus, water holding capacity, gel strength, in vitro digestion, digestibility
MeSH terms
Animals, Digestion, Food Handling, Gels, Hot Temperature, Muscle Proteins, Myofibrils, Pressure, Protein Conformation, Tuna, Seafood, Fish Proteins

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