The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin
Open accessAbstract
Black soldier fly (BSF, Hermetia illucens) constitutes an economic way to convert residual biomasses into a valuable source of biomolecules, such as proteins, lipids and chitin. The present investigation was undertaken to evaluate the feasibility of applying different extraction protocols, either chemical extractions or enzymatic assisted extraction, to recover pure fat, protein and chitin fractions. First, exact proximate composition, total amino acids, fatty acids profile, and N-acetylglucosamine content of the prepupae samples were determined. BSF prepupae biomass contained, expressed on dry weight, 32% proteins, 37% lipids, 19% minerals, 9% chitin. The lipid fraction was easily recovered by organic solvents, while the most challenging issue was the separation of protein from chitin. The best separation was obtained by alkali extraction of proteins (96% of protein recovered) albeit with loss in their integrity as indicated by the measurement of the degree of hydrolysis with the o-phthaldialdehyde method. To avoid protein damage in alkali media, a stepwise protein extraction adopting milder conditions was also explored based on Osborne fractionation method, allowing the recovery of >85% of BSF high purity and high quality proteins, and the obtainment of chitin-enriched fraction as well. The possibility of using an enzymatic assisted extraction of proteins was also explored, obtaining a maximum nitrogen solubilisation in the best case (with Bacillus licheniformis protease) of about 60%. In this latter case, the chitin fraction obtained also had a significant residual protein content.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Chitin and chitosan polymers: Chemistry, solubility and fiber formation
Pillai, C. K. S., Paul, W., & Sharma, C. P. (2009) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2009.04.001 · Cited by 2,745
Spranghers, T., Ottoboni, M., Klootwijk, C., Ovyn, A., Deboosere, S., De Meulenaer, B., Michiels, J., Eeckhout, M., De Clercq, P., & De Smet, S. (2017) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.8081 · Cited by 970
Janssen, R. H., Vincken, J.-P., van den Broek, L. A. M., Fogliano, V., & Lakemond, C. M. M. (2017) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.7b00471 · Cited by 813
The use of fly larvae for organic waste treatment
Čičková, H., Newton, G. L., Lacy, R. C., & Kozánek, M. (2015) · Waste Management · doi:10.1016/j.wasman.2014.09.026 · Cited by 624
Extraction and characterisation of protein fractions from five insect species
Yi, L., Lakemond, C. M. M., Sagis, L. M. C., Eisner-Schadler, V., van Huis, A., & van Boekel, M. A. J. S. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.05.115 · Cited by 620
(2011) · doi:10.1007/s12649-011-9079-1 · Cited by 570
A value added manure management system using the black soldier fly
Craig Sheppard, D., Larry Newton, G., Thompson, S. A., & Savage, S. (1994) · Bioresource Technology · doi:10.1016/0960-8524(94)90102-3 · Cited by 508
Rearing Methods for the Black Soldier Fly (Diptera: Stratiomyidae): Table 1
Sheppard, D. C., Tomberlin, J. K., Joyce, J. A., Kiser, B. C., & Sumner, S. M. (2002) · Journal of Medical Entomology · doi:10.1603/0022-2585-39.4.695 · Cited by 479
The proteins of the wheat kernel
Osborne, T. B. (1907) · doi:10.5962/bhl.title.26152 · Cited by 471
Spellman, D., McEvoy, E., O’Cuinn, G., & FitzGerald, R. J. (2003) · International Dairy Journal · doi:10.1016/s0958-6946(03)00053-0 · Cited by 422
Cited by
Insects as food and feed, a new emerging agricultural sector: a review
van Huis, A. (2020) · Journal of Insects as Food and Feed · doi:10.3920/jiff2019.0017 · Cited by 544
Singh, A., & Kumari, K. (2019) · Journal of Environmental Management · doi:10.1016/j.jenvman.2019.109569 · Cited by 229
Abd El-Hack, M., Shafi, M., Alghamdi, W., Abdelnour, S., Shehata, A., Noreldin, A., Ashour, E., Swelum, A., Al-Sagan, A., Alkhateeb, M., et al. (2020) · Agriculture · doi:10.3390/agriculture10080339 · Cited by 208
Smets, R., Verbinnen, B., Van De Voorde, I., Aerts, G., Claes, J., & Van Der Borght, M. (2020) · Waste and Biomass Valorization · doi:10.1007/s12649-019-00924-2 · Cited by 162
Hermetia illucens (L.) larvae as chicken manure management tool for circular economy
Bortolini, S., Macavei, L. I., Saadoun, J. H., Foca, G., Ulrici, A., Bernini, F., Malferrari, D., Setti, L., Ronga, D., & Maistrello, L. (2020) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2020.121289 · Cited by 149
Self-citation
Metadata
- Authors
- Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., Dossena, A., & Sforza, S.
- Venue
- Food Research International
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 105
- Pages
- 812-820
- ISSN
- 0963-9969 (ISSN-L)
- OpenAlex
- W2773507405
- Cited by
- 348
- References
- 33
- FWCI
- 18.02
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin. https://app.cochat.ai/papers/10.1016/j.foodres.2017.12.012
- The paper
- Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., Dossena, A., & Sforza, S. (2018). Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin. Food Research International. https://doi.org/10.1016/j.foodres.2017.12.012
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
