The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Limitations of oxygen delivery to cells in culture: An underappreciated problem in basic and translational research
Open accessAbstract
Molecular oxygen is one of the most important variables in modern cell culture systems. Fluctuations in its concentration can affect cell growth, differentiation, signaling, and free radical production. In order to maintain culture viability, experimental validity, and reproducibility, it is imperative that oxygen levels be consistently maintained within physiological "normoxic" limits. Use of the term normoxia, however, is not consistent among scientists who experiment in cell culture. It is typically used to describe the atmospheric conditions of a standard incubator, not the true microenvironment to which the cells are exposed. This error may lead to the situation where cells grown in a standard "normoxic" oxygen concentration may actually be experiencing a wide range of conditions ranging from hyperoxia to near-anoxic conditions at the cellular level. This apparent paradox is created by oxygen's sluggish rate of diffusion through aqueous medium, and the generally underappreciated effects that cell density, media volume, and barometric pressure can have on pericellular oxygen concentration in a cell culture system. This review aims to provide an overview of this phenomenon we have termed "consumptive oxygen depletion" (COD), and includes a basic review of the physics, potential consequences, and alternative culture methods currently available to help circumvent this largely unrecognized problem.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Specifically, O2 first dissolves at the air-medium interface and then diffuses through the liquid (at least several millimeters) to reach and oxygenate cell microenvironments (Place et al., 2017) even as cells undergo exponential growth.”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
The cited source says what the claim attributes to it.
In the citing paper
“In concert, these processes interact to create a changing environmental gradient from the surface of the medium down to the microenvironment of the cells (Place et al., 2017).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
The cited source says what the claim attributes to it.
In the citing paper
“In the case of O2, medium deoxygenation is caused by the disparity between rates of O2 consumption via cellular metabolism and the replenishment of O2 at the air-medium interface (Place et al., 2017; Al-Ani et al., 2018).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Ki-67 protein: From the known and the unknown
Scholzen, T. E., & Gerdes, J. (2000) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(200003)182:3 · Cited by 4,707
Kim, J.-W., Tchernyshyov, I., Semenza, G. L., & Dang, C. V. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.02.002 · Cited by 3,905
HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption
Papandreou, I., Cairns, R. A., Fontana, L., Lim, A. L., & Denko, N. C. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.01.012 · Cited by 2,267
THE THEORY AND APPLICATIONS OF THE EXCHANGE OF INERT GAS AT THE LUNGS AND TISSUES
Kety, S. S. (1951) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)07246-1 · Cited by 1,665
Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices
Halldorsson, S., Lucumi, E., Gómez-Sjöberg, R., & Fleming, R. M. T. (2015) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.07.029 · Cited by 1,027
(1943) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/4.2.165 · Cited by 833
Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor
Hirsilä, M., Koivunen, P., Günzler, V., Kivirikko, K. I., & Myllyharju, J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304982200 · Cited by 794
Krogh, A. (1919) · The Journal of Physiology · doi:10.1113/jphysiol.1919.sp001838 · Cited by 633
The Multiple Origins of Complex Multicellularity
Knoll, A. H. (2011) · Annual Review of Earth and Planetary Sciences · doi:10.1146/annurev.earth.031208.100209 · Cited by 562
The supply of oxygen to the tissues and the regulation of the capillary circulation
Krogh, A. (1919) · The Journal of Physiology · doi:10.1113/jphysiol.1919.sp001844 · Cited by 530
Cited by
Sorted by citations
Items 101–200 of 434
Kurniawan, D. A., Leo, S., Inamatsu, M., Funaoka, S., Aihara, T., Aiko, M. C., Rei, I., Sakura, T., Arakawa, H., Kato, Y., et al. (2024) · PNAS Nexus · doi:10.1093/pnasnexus/pgae070 · Cited by 23
Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches
Farré, R., Almendros, I., Montserrat, J. M., Gozal, D., & Navajas, D. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.01803 · Cited by 23
Boylin, K., Aquino, G. V., Purdon, M., Abedi, K., Kasendra, M. J., & Barrile, R. (2024) · Biofabrication · doi:10.1088/1758-5090/ad4c08 · Cited by 21
UVC-Emitting LuPO4:Pr3+ Nanoparticles Decrease Radiation Resistance of Hypoxic Cancer Cells
Müller, M., Espinoza, S., Jüstel, T., Held, K. D., Anderson, R. R., & Purschke, M. (2019) · Radiation Research · doi:10.1667/rr15491.1 · Cited by 22
Callaghan, N. I., Lee, S., Hadipour‐Lakmehsari, S., Lee, X., Siraj, M. A., Driouchi, A., Yip, C. M., Husain, M., Simmons, C. A., & Gramolini, A. O. (2020) · Communications Biology · doi:10.1038/s42003-020-0946-9 · Cited by 22
Electrochemical laser induced graphene-based oxygen sensor
Hossain, M. F., McCracken, S., & Slaughter, G. E. (2021) · Journal of Electroanalytical Chemistry · doi:10.1016/j.jelechem.2021.115690 · Cited by 22
Gas-modulating microcapsules for spatiotemporal control of hypoxia
Molley, T. G., Jiang, S., Ong, L. J. Y., Kopecky, C., Ranaweera, C. D., Jalandhra, G. K., Milton, L. A., Kardia, E., Zhou, Z., Rnjak‐Kovacina, J., et al. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2217557120 · Cited by 25
Soft Biological Actuators for Meter-Scale Homeostatic Biohybrid Robots
Heisser, R. H., Bawa, M., Shah, J., Bu, A., & Raman, R. (2025) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00785 · Cited by 23
Mimicking and surpassing the xenograft model with cancer-on-chip technology
Komen, J., van Neerven, S. M., van den Berg, A., Vermeulen, L., & van der Meer, A. D. (2021) · EBioMedicine · doi:10.1016/j.ebiom.2021.103303 · Cited by 21
Li, W., Jia, M. X., Deng, J., Wang, J. H., Zuberi, Z., Yang, S., Ba, J., & Zhu, C. Y. (2020) · Cancers · doi:10.3390/cancers12051236 · Cited by 21
Sugimoto, H., Murahashi, Y., Chijimatsu, R., Miwa, S., Yano, F., Tanaka, S., & Saito, T. (2020) · Biomedical Research · doi:10.2220/biomedres.41.43 · Cited by 20
Multi-cellular aggregates, a model for living matter
Ackermann, J., Amar, M. B., & Joanny, J. (2021) · Physics Reports · doi:10.1016/j.physrep.2021.05.001 · Cited by 24
Hypoxic preconditioning in renal ischaemia–reperfusion injury: a review in pre-clinical models
Bruzzese, L., Lumet, G., Vairo, D., Guiol, C., Guieu, R., & Faure, A. (2021) · Clinical Science · doi:10.1042/cs20210615 · Cited by 21
Scalable fabrication, compartmentalization and applications of living microtissues
Schot, M., Araújo‐Gomes, N., van Loo, B., Kamperman, T., & Leijten, J. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.04.005 · Cited by 21
Schumacher, A., Roumans, N. J. T., Rademakers, T., Joris, V., Eischen-Loges, M. J., van Griensven, M., & LaPointe, V. L. S. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.860138 · Cited by 20
Mitochondrial respiration reduces exposure of the nucleus to oxygen
Mori, M. P., Penjweini, R., Ma, J., Alspaugh, G., Andreoni, A., Kim, Y., Wang, P., Knutson, J. R., & Hwang, P. M. (2023) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2023.103018 · Cited by 19
Zablotskii, V., Polyakova, T., & Dejneka, A. (2020) · Bioelectromagnetics · doi:10.1002/bem.22309 · Cited by 20
A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
Martinez, C.-A., Cistulli, P. A., & Cook, K. M. (2019) · BIO-PROTOCOL · doi:10.21769/bioprotoc.3371 · Cited by 19
Sphabmixay, P., Raredon, M. S. B., Wang, A. J.-S., Lee, H., Hammond, P. T., Fang, N. X., & Griffith, L. G. (2021) · Biofabrication · doi:10.1088/1758-5090/ac23aa · Cited by 20
A DNA-structured mathematical model of cell-cycle progression in cyclic hypoxia
Celora, G. L., Bader, S. B., Hammond, E. M., Maini, P. K., Pitt‐Francis, J., & Byrne, H. M. (2022) · Journal of Theoretical Biology · doi:10.1016/j.jtbi.2022.111104 · Cited by 17
Farré, R., Almendros, I., Martínez‐García, M. Á., & Gozal, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232214430 · Cited by 21
Kim, D., Nguyen, Q. T. T., Lee, S., Choi, K.-M., Lee, E., & Park, J. Y. (2023) · npj Microgravity · doi:10.1038/s41526-023-00311-1 · Cited by 19
Using the heme peroxidase APEX2 to probe intracellular H2O2 flux and diffusion
Eid, M., Barayeu, U., Sulková, K., Aranda-Vallejo, C., & Dick, T. P. (2024) · Nature Communications · doi:10.1038/s41467-024-45511-9 · Cited by 19
Oxygenation as a driving factor in epithelial differentiation at the air–liquid interface
Kouthouridis, S., Goepp, J., Martini, C., Matthes, E., Hanrahan, J. W., & Moraes, C. (2021) · Integrative Biology · doi:10.1093/intbio/zyab002 · Cited by 18
Raab, N., Zeh, N., Schlossbauer, P., Mathias, S., Lindner, B., Stadermann, A., Gamer, M., Fischer, S., Holzmann, K., Handrick, R., et al. (2021) · New Biotechnology · doi:10.1016/j.nbt.2021.10.005 · Cited by 19
Wesseler, M. F., Johansen, M. N., Kızıltay, A., Mortensen, K. I., & Larsen, N. B. (2022) · Lab on a Chip · doi:10.1039/d2lc00063f · Cited by 19
Building blocks of microphysiological system to model physiology and pathophysiology of human heart
Vuorenpää, H., Björninen, M., Välimäki, H., Ahola, A., Kroon, M., Honkamäki, L., Koivumäki, J. T., & Pekkanen-Mattila, M. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1213959 · Cited by 23
Wulftange, W. J., Rose, M. A., Garmendia‐Cedillos, M., da Silva, D., Poprawski, J. E., Srinivasachar, D., Sullivan, T., Lim, L., Bliskovsky, V. V., Hall, M. D., et al. (2019) · Journal of Cellular Physiology · doi:10.1002/jcp.28665 · Cited by 21
DNA mismatch repair protects the genome from oxygen-induced replicative mutagenesis
Lózsa, R., Németh, E., Gervai, J. Z., Márkus, B. G., Kollarics, S., Gyüre, Z., Tóth, J. S., Simon, F., & Szüts, D. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad775 · Cited by 17
HIF-stabilizing biomaterials: from hypoxia-mimicking to hypoxia-inducing
Colombani, T., Bhatt, K., Epel, B., Kotecha, M., & Bencherif, S. A. (2023) · Materials Advances · doi:10.1039/d3ma00090g · Cited by 17
Rogers, Z. J., Colombani, T., Khan, S., Bhatt, K., Nukovic, A., Zhou, G., Woolston, B. M., Taylor, C. T., Gilkes, D. M., Slavov, N., et al. (2024) · Advanced Science · doi:10.1002/advs.202402557 · Cited by 20
Cristea, D., Krishtul, S., Kuppusamy, P., Baruch, L., Machluf, M., & Blank, A. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.10.032 · Cited by 17
Microwell bag culture for large-scale production of homogeneous islet-like clusters
Suenaga, R., Konagaya, S., Yamaura, J., Ito, R., Tanaka, S., Ishizaki, Y., & Toyoda, T. (2022) · Scientific Reports · doi:10.1038/s41598-022-09124-w · Cited by 16
Pachane, B. C., Nunes, A. C. C., Cataldi, T. R., Micocci, K. C., Moreira, B. C., Labate, C. A., Selistre-de-Araújo, H. S., & Altei, W. F. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232012646 · Cited by 15
Soft bioreactor systems: a necessary step toward engineered MSK soft tissue?
Dvorak, N., Liu, Z., & Mouthuy, P. (2024) · Frontiers in Robotics and AI · doi:10.3389/frobt.2024.1287446 · Cited by 17
Generation, interrogation, and future applications of microglia-containing brain organoids
Stefano, J. D., Marco, F. D., Cicalini, I., FitzGerald, U., Pieragostino, D., Verhoye, M., Ponsaerts, P., & Breedam, E. V. (2024) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-00921 · Cited by 17
Dinger, T. F., Chen, O., Dittfeld, C., Hetze, L., Hüther, M., Wondrak, M., Löck, S., Eicheler, W., Breier, G., & Kunz‐Schughart, L. A. (2020) · Theranostics · doi:10.7150/thno.39893 · Cited by 16
Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors
Gallego‐Murillo, J. S., Iacono, G., van der Wielen, L. A. M., van den Akker, E., Lindern, M. V., & Wahl, A. (2022) · Biotechnology and Bioengineering · doi:10.1002/bit.28193 · Cited by 16
A cryopreservation method for bioengineered 3D cell culture models
Herrero-Gómez, A., Azagra, M., & Marco‐Rius, I. (2022) · Biomedical Materials · doi:10.1088/1748-605x/ac76fb · Cited by 15
Wagner, K. T., Lu, R. X. Z., Landau, S., Shawky, S. A., Zhao, Y., Bodenstein, D. F., Vargas, L. F. J., Jiang, R., Okhovatian, S., Wang, Y., et al. (2024) · Biofabrication · doi:10.1088/1758-5090/ad76d9 · Cited by 17
Koochana, P. K., Mohanty, A., Parida, A., Behera, N., Behera, P. M., Dixit, A., & Behera, R. K. (2021) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-021-01850-2 · Cited by 17
Abreu, C. M., Reis, R. L., & Marques, A. P. (2021) · Cell Proliferation · doi:10.1111/cpr.13013 · Cited by 16
Real-Time Monitoring of Oxygen Levels within Thermoplastic Organ-on-Chip Devices
Bussooa, A., Tubbs, E., Revol-Cavalier, F., Chmayssem, A., Alessio, M., Cosnier, M.-L., & Verplanck, N. (2022) · Biosensors and Bioelectronics X · doi:10.1016/j.biosx.2022.100198 · Cited by 15
Chelenga, M., Sakaguchi, K., Abdel‐Ghani, M. A., Yanagawa, Y., Katagiri, S., & Nagano, M. (2020) · Theriogenology · doi:10.1016/j.theriogenology.2020.07.023 · Cited by 15
Capuana, E., Testa, M., Marco, C. D., Lopresti, F., & Carrubba, V. L. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202500378 · Cited by 14
Bioreactor parameters and systems for cultured meat production
Ngwa, C. J., Chiu, K., Brenner, K. J., Rodrigues, C. A. V., Peršin, Z., Vajda, J., Vihar, B., Morales-Dalmau, J., Ferreira, F. C., Henkel, M., et al. (2025) · Future Foods · doi:10.1016/j.fufo.2025.100796 · Cited by 14
Han, J., & Zúñiga‐Pflücker, J. C. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.652665 · Cited by 19
Amiri, F., Chamgordani, S. Z., & Ghourchian, H. (2024) · Scientific Reports · doi:10.1038/s41598-024-70003-7 · Cited by 14
Selvam, A. K., Wiklander, R. J., Gramignoli, R., Achour, A., Salter, H., & Björnstedt, M. (2021) · Antioxidants · doi:10.3390/antiox10071094 · Cited by 15
Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity
Xiao, C., Hull, D., Qiu, S., Yeung, J., Zheng, J., Barwell, T., Robertson, R. M., & Seroude, L. (2019) · G3 Genes Genomes Genetics · doi:10.1534/g3.119.400782 · Cited by 14
Generation of hypoxia-sensing chimeric antigen receptor T cells
Kosti, P., Larios-Martinez, K. I., Maher, J. F., & Arnold, J. N. (2021) · STAR Protocols · doi:10.1016/j.xpro.2021.100723 · Cited by 13
McDonnell, E. E., & Buckley, C. T. (2022) · JOR Spine · doi:10.1002/jsp2.1222 · Cited by 13
Fu, P., Zhu, R., Gao, W., & Gong, L. (2023) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.18096 · Cited by 12
Sacrificial strategy towards the formation of vascular‐like networks in volumetric tissue constructs
Buckley, C., Ibrahim, R., Giordano, F., Xu, N., Sems, B., & Wang, H. (2024) · BMEMat · doi:10.1002/bmm2.12118 · Cited by 13
Mahdavi, R., Hashemi‐Najafabadi, S., Ghiass, M. A., Valaskivi, S., Välimäki, H., Kreutzer, J., Blomqvist, C. H., Romeo, S., Kallio, P. J., & Adiels, C. B. (2025) · Biomedical Microdevices · doi:10.1007/s10544-025-00738-1 · Cited by 13
Wang, Y., Sims, C. E., & Allbritton, N. L. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c02016 · Cited by 13
Lee, K., Kim, D., & Kim, W. (2021) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2021.02.113 · Cited by 14
Burton, L., Scaife, P. J., Paine, S. W., Mellor, H. R., Abernethy, L., Littlewood, P., & Rauch, C. (2020) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00472.2019 · Cited by 13
The Redox-Metabolic Couple of T Lymphocytes: Potential Consequences for Hypertension
Moshfegh, C. M., & Case, A. J. (2020) · Antioxidants and Redox Signaling · doi:10.1089/ars.2020.8042 · Cited by 13
Self-citation96-Well Oxygen Control Using a 3D-Printed Device
Szmelter, A., Jacob, J., & Eddington, D. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.0c04627 · Cited by 13
Iron nitrosyl complexes are formed from nitrite in the human placenta
Mukosera, G. T., Principe, P., Mata‐Greenwood, E., Liu, T., Schroeder, H. J., Parast, M. M., & Blood, A. B. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.102078 · Cited by 11
Nguyen, C. T., Duong, V. T., Hwang, C. H., & Koo, K.-I. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v8i3.557 · Cited by 12
Remmers, S., van der Heijden, F. C., Ito, K., & Hofmann, S. (2022) · Bone Reports · doi:10.1016/j.bonr.2022.101651 · Cited by 12
Alkildani, S., Ren, Y., Liu, L., Rimashevskiy, D. V., Schnettler, R., Radenković, M. B., Najman, S. J., Stojanović, S., Jung, O., & Barbeck, M. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24076833 · Cited by 12
Microfluidic design for in-vitro liver zonation—a numerical analysis using COMSOL Multiphysics
Mahdavi, R., Hashemi‐Najafabadi, S., Ghiass, M. A., & Adiels, C. B. (2023) · Medical & Biological Engineering & Computing · doi:10.1007/s11517-023-02936-6 · Cited by 12
Kingsley, J., Costello, J. R., Raghunand, N., & Rejniak, K. A. (2021) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1009206 · Cited by 12
In Situ Crosslinkable Collagen-Based Hydrogels for 3D Printing of Dermis-Mimetic Constructs
Kang, M. S., Kwon, M., Lee, S. Y., Lee, S. H., Jo, H. J., Kim, B., Kim, K. S., & Han, D. (2022) · ECS Journal of Solid State Science and Technology · doi:10.1149/2162-8777/ac6897 · Cited by 12
Lee, J., Kim, E., Chong, K., Ryu, S., Kim, C., Choi, K., Kim, J.-H., & Choi, C. (2022) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-022-04529-2 · Cited by 12
Brochado, A. C. B., Costa-Silva, D., da Silva, J. C., Lowenstein, A., Gameiro, V. S., Mavropoulos, E., Mourão, C. F., & Alves, G. G. (2023) · Applied Sciences · doi:10.3390/app13031602 · Cited by 10
Dorrigiv, D., Goyette, P.-A., St‐Georges‐Robillard, A., Mes‐Masson, A., & Gervais, T. (2023) · Cancers · doi:10.3390/cancers15041060 · Cited by 12
Reactive oxygen species may cause amyloid beta aggregation by free radical polymerization
Majid, A., & Garg, S. (2024) · Journal of Applied Polymer Science · doi:10.1002/app.55190 · Cited by 11
Generating human skeletal myoblast spheroids for vascular myogenic tissue engineering
Minne, M., Terrie, L., Wüst, R., Hasevoets, S., Kerchove, K. V., Nimako, K., Lambrichts, I., Thorrez, L., & Declercq, H. (2024) · Biofabrication · doi:10.1088/1758-5090/ad2fd5 · Cited by 11
Liu, Y., Suhail, Y., Novin, A., Afzal, J. M., Pant, A., & Gupta, K. (2024) · Human Cell · doi:10.1007/s13577-024-01046-1 · Cited by 10
Reaction of peroxynitrite with thiols, hydrogen sulfide and persulfides
Trujillo, M., Cuevasanta, E., Turell, L., Benchoam, D., Ferrer‐Sueta, G., Zeida, A., Quijano, C., Carballal, S., Radí, R., & Álvarez, B. (2024) · Redox Biochemistry and Chemistry · doi:10.1016/j.rbc.2024.100039 · Cited by 12
Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate
Yao, M., Sattler, T., Rabbani, Z. N., Pulliam, T. H., Walker, G. M., & Gamcsik, M. P. (2018) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00276.2018 · Cited by 11
Jones, M., Nankervis, B., Roballo, K. C. S., Pham, H., Bushman, J. S., & Coeshott, C. M. (2020) · Cell Transplantation · doi:10.1177/0963689720923578 · Cited by 11
Pezet, M. G., Gómez-Durán, A., Klimm, F., Aryaman, J., Burr, S. P., Wei, W., Saitou, M., Prudent, J., & Chinnery, P. F. (2021) · Communications Biology · doi:10.1038/s42003-021-02069-2 · Cited by 11
Effect of nutrient metabolism on cartilaginous tissue formation
Tarantino, R., Chiu, L. L. Y., Weber, J. F., Tse, M. Y., Bardana, D. D., Pang, S. C.-N., & Waldman, S. D. (2021) · Biotechnology and Bioengineering · doi:10.1002/bit.27888 · Cited by 11
Tedjo, W., Obeidat, Y., Catandi, G. D., Carnevale, E. M., & Chen, T. W. (2021) · Biosensors · doi:10.3390/bios11080256 · Cited by 11
Redox biology and electrochemistry. Towards evaluation of bioactive electron donors and acceptors
Vacek, J., Zatloukalová, M., & Kabeláč, M. (2022) · Current Opinion in Electrochemistry · doi:10.1016/j.coelec.2022.101142 · Cited by 11
Airway Basal Cells Mediate Hypoxia-Induced EMT by Increasing Ribosome Biogenesis
Hou, Y., Ding, Y. P., Du, D., Yu, T., Zhou, W., Cui, Y., & Nie, H. (2021) · Frontiers in Pharmacology · doi:10.3389/fphar.2021.783946 · Cited by 11
Callaghan, N. I., Durland, L. J., Ireland, R. G., Santerre, J. P., Simmons, C. A., & Huyer, L. D. (2022) · npj Regenerative Medicine · doi:10.1038/s41536-022-00246-3 · Cited by 10
Dittfeld, C., Winkelkotte, M., Scheer, A., Voigt, E., Schmieder, F., Behrens, S., Jannasch, A., Matschke, K., Sonntag, F., & Tugtekin, S. (2023) · Journal of Biological Engineering · doi:10.1186/s13036-023-00377-1 · Cited by 10
Fragomeni, G., NAPOLI, L. D., Gregorio, V. D., Genovese, V., Barbato, V., Serratore, G., Morrone, G., Travaglione, A., Candela, A., Gualtieri, R., et al. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1310696 · Cited by 10
Oxygen control in cell culture – Your cells may not be experiencing what you think!
Rogers, Z. J., Flood, D., Bencherif, S. A., & Taylor, C. T. (2024) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2024.11.036 · Cited by 13
Kasmi, N., Pieruccioni, L., Pitot, E., Fourquaux, I., Wodrinski, A., Gibot, L., & Fitremann, J. (2025) · Journal of Materials Chemistry B · doi:10.1039/d4tb02658f · Cited by 10
Vervust, W., Safaei, S., Witschas, K., Leybaert, L., & Ghysels, A. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2422437122 · Cited by 10
Liquid marble microbioreactor aeration facilitated by on-demand electrolysis
Lin, E. S., Song, Z., Ong, J. W., Abid, H. A., Liew, O. W., & Ng, T. W. (2022) · Results in Chemistry · doi:10.1016/j.rechem.2022.100334 · Cited by 10
Hypothermia Alleviates Reductive Stress, a Root Cause of Ischemia Reperfusion Injury
Eskla, K., Vellama, H., Tarve, L., Eichelmann, H. Y., Jagomäe, T., Porosk, R., Oja, V., Rämma, H., Peet, N., Laisk, A., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231710108 · Cited by 10
Louw, E.-V., Liebenberg, W., Willers, C., Dube, A., Aucamp, M. E., & Gerber, M. (2022) · Drug Delivery and Translational Research · doi:10.1007/s13346-022-01267-7 · Cited by 10
Submersion and hypoxia inhibit alveolar epithelial Na+ transport through ERK/NF-κB signaling pathway
Zhou, W., Hou, Y., Yu, T., Wang, T., Ding, Y. P., & Nie, H. (2023) · Respiratory Research · doi:10.1186/s12931-023-02428-z · Cited by 10
Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments
Yao, M., Walker, G. M., & Gamcsik, M. P. (2023) · Cytotechnology · doi:10.1007/s10616-023-00584-0 · Cited by 10
Modeling Preclinical Cancer Studies under Physioxia to Enhance Clinical Translation
Adebayo, A. K., & Nakshatri, H. (2022) · Cancer Research · doi:10.1158/0008-5472.can-22-2311 · Cited by 12
Lamela, F., Bologna‐Molina, R. E., Parietti, F., Pereira‐Prado, V., Millán, M., Silva, A., Llaguno, J. M., Alonso, J. R., Fernández, A., Sotelo-Silveira, J. R., et al. (2024) · Tissue and Cell · doi:10.1016/j.tice.2024.102408 · Cited by 10
Chronic hypoxia for the adaptation of extracellular vesicle phenotype
Pridgeon, C. S., Airavaara, K., Monola, J., Jokela, A., Palmer, D. H., Yliperttula, M. L., & Harjumäki, R. (2024) · Scientific Reports · doi:10.1038/s41598-024-73453-1 · Cited by 10
Daumová, L., Manakov, D., Petrák, J., Sovilj, D., Běhounek, M., Anděra, L., Vít, O., Součková, O., Havránek, O., Dolníková, A., et al. (2025) · Cell Death Discovery · doi:10.1038/s41420-025-02341-y · Cited by 10
Hyperbaric oxygen therapy induces transcriptome changes in elderly: a prospective trial
Hadanny, A., Forer, R., Volodarsky, D., Daniel-Kotovsky, M., Catalogna, M., Zemel, Y., Bechor, Y., & Efrati, S. (2021) · Aging · doi:10.18632/aging.203709 · Cited by 9
Sampson, K., Koo, S., Gadola, C., Vasiukhina, A., Singh, A., Spartano, A., Gollapudi, R., Duley, M. L., Mueller, J., James, P. F., et al. (2021) · SN Applied Sciences · doi:10.1007/s42452-021-04871-3 · Cited by 9
Mathematical modelling of oxygen transport in a muscle-on-chip device
Hardman, D. T. A., Nguyen, M., Descroix, S., & Bernabéu, M. O. (2022) · Interface Focus · doi:10.1098/rsfs.2022.0020 · Cited by 9
Optimizing the Cell Culture Microenvironment
Bertoncello, I. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9086-3_2 · Cited by 9
Metadata
- Authors
- Place, T. L., Domann, F. E., & Case, A. J.
- Affiliations
- University of Iowa (US)
- University of Nebraska Medical Center (US)
- Corresponding author
- Domann, Frederick E.
- Case, Adam J.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 113
- Pages
- 311-322
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC5699948
- OpenAlex
- W2762502468
- Cited by
- 434
- References
- 74
- Citations by year
- 2026: 50 · 2025: 50 · 2024: 68 · 2023: 47 · 2022: 69 · 2021: 72 · 2020: 46 · 2019: 19 · 2018: 12
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Update notices
- erratum (2021-01-01)
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Free Radical Biology and Medicine (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R00HL123471)
- National Cancer Institute (P30CA086862)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Physiology (medical) › Biomedical and Chemical Research
- Keywords
- oxygen delivery, cell culture, normoxia, hyperoxia, anoxia, oxygen diffusion, cell density, atmospheric pressure, culture vessel design, reproducibility
- MeSH terms
- Cell Count, Cell Differentiation, Cells, Cultured, Diffusion, Humans, Oxygen, Oxygen Consumption, Reproducibility of Results, Cell Hypoxia, Hyperoxia, Cell Culture Techniques, Cell Proliferation, Translational Research, Biomedical
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
