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Limitations of oxygen delivery to cells in culture: An underappreciated problem in basic and translational research
Open accessAbstract
Molecular oxygen is one of the most important variables in modern cell culture systems. Fluctuations in its concentration can affect cell growth, differentiation, signaling, and free radical production. In order to maintain culture viability, experimental validity, and reproducibility, it is imperative that oxygen levels be consistently maintained within physiological "normoxic" limits. Use of the term normoxia, however, is not consistent among scientists who experiment in cell culture. It is typically used to describe the atmospheric conditions of a standard incubator, not the true microenvironment to which the cells are exposed. This error may lead to the situation where cells grown in a standard "normoxic" oxygen concentration may actually be experiencing a wide range of conditions ranging from hyperoxia to near-anoxic conditions at the cellular level. This apparent paradox is created by oxygen's sluggish rate of diffusion through aqueous medium, and the generally underappreciated effects that cell density, media volume, and barometric pressure can have on pericellular oxygen concentration in a cell culture system. This review aims to provide an overview of this phenomenon we have termed "consumptive oxygen depletion" (COD), and includes a basic review of the physics, potential consequences, and alternative culture methods currently available to help circumvent this largely unrecognized problem.
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“Specifically, O2 first dissolves at the air-medium interface and then diffuses through the liquid (at least several millimeters) to reach and oxygenate cell microenvironments (Place et al., 2017) even as cells undergo exponential growth.”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
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“In concert, these processes interact to create a changing environmental gradient from the surface of the medium down to the microenvironment of the cells (Place et al., 2017).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
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“In the case of O2, medium deoxygenation is caused by the disparity between rates of O2 consumption via cellular metabolism and the replenishment of O2 at the air-medium interface (Place et al., 2017; Al-Ani et al., 2018).”
From Toward Best Practices for Controlling Mammalian Cell Culture Environments (2022) · cites this paper as Place et al., 2017
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The Ki-67 protein: From the known and the unknown
Scholzen, T. E., & Gerdes, J. (2000) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(200003)182:3 · Cited by 4,707
Kim, J.-W., Tchernyshyov, I., Semenza, G. L., & Dang, C. V. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.02.002 · Cited by 3,905
HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption
Papandreou, I., Cairns, R. A., Fontana, L., Lim, A. L., & Denko, N. C. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.01.012 · Cited by 2,267
THE THEORY AND APPLICATIONS OF THE EXCHANGE OF INERT GAS AT THE LUNGS AND TISSUES
Kety, S. S. (1951) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)07246-1 · Cited by 1,665
Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices
Halldorsson, S., Lucumi, E., Gómez-Sjöberg, R., & Fleming, R. M. T. (2015) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.07.029 · Cited by 1,027
(1943) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/4.2.165 · Cited by 833
Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor
Hirsilä, M., Koivunen, P., Günzler, V., Kivirikko, K. I., & Myllyharju, J. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304982200 · Cited by 794
Krogh, A. (1919) · The Journal of Physiology · doi:10.1113/jphysiol.1919.sp001838 · Cited by 633
The Multiple Origins of Complex Multicellularity
Knoll, A. H. (2011) · Annual Review of Earth and Planetary Sciences · doi:10.1146/annurev.earth.031208.100209 · Cited by 562
The supply of oxygen to the tissues and the regulation of the capillary circulation
Krogh, A. (1919) · The Journal of Physiology · doi:10.1113/jphysiol.1919.sp001844 · Cited by 530
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Dalfino, S., Savadori, P., Piazzoni, M., Connelly, S., Giannì, A. B., FABBRO, M. D., Tartaglia, G. M., & Moroni, L. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300128 · Cited by 50
Kishimoto, S., Brender, J., Chandramouli, G. V. R., Saida, Y., Yamamoto, K., Mitchell, J. B., & Krishna, M. C. (2020) · Antioxidants and Redox Signaling · doi:10.1089/ars.2020.8131 · Cited by 48
Gavert, N. S., Zwang, Y., Weiser, R., Greenberg, O., Halperin, S., Jacobi, O., Mallel, G., Sandler, O., Berger, A. J., Stossel, E., et al. (2022) · Nature Cancer · doi:10.1038/s43018-021-00325-2 · Cited by 46
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Tanumihardja, E., Slaats, R. H., van der Meer, A. D., Passier, R. C., Olthuis, W., & van den Berg, A. (2020) · ACS Sensors · doi:10.1021/acssensors.0c02282 · Cited by 48
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Metadata
- Authors
- Place, T. L., Domann, F. E., & Case, A. J.
- Affiliations
- University of Iowa (US)
- University of Nebraska Medical Center (US)
- Corresponding author
- Domann, Frederick E.
- Case, Adam J.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 113
- Pages
- 311-322
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC5699948
- OpenAlex
- W2762502468
- Cited by
- 434
- References
- 74
- Citations by year
- 2026: 50 · 2025: 50 · 2024: 68 · 2023: 47 · 2022: 69 · 2021: 72 · 2020: 46 · 2019: 19 · 2018: 12
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Update notices
- erratum (2021-01-01)
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Free Radical Biology and Medicine (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R00HL123471)
- National Cancer Institute (P30CA086862)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Health Sciences › Medicine › Physiology (medical) › Biomedical and Chemical Research
- Keywords
- oxygen delivery, cell culture, normoxia, hyperoxia, anoxia, oxygen diffusion, cell density, atmospheric pressure, culture vessel design, reproducibility
- MeSH terms
- Cell Count, Cell Differentiation, Cells, Cultured, Diffusion, Humans, Oxygen, Oxygen Consumption, Reproducibility of Results, Cell Hypoxia, Hyperoxia, Cell Culture Techniques, Cell Proliferation, Translational Research, Biomedical
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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