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Cited by 378

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Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications

Open access
Pereira, Jorge F.B., Gudiña, Eduardo J., Costa, Rita, Vitorino, Rui, Teixeira, José A., Coutinho, João A.P., Rodrigues, Lígia R.·2013·Fuel

Abstract

Biosurfactant production by three Bacillus subtilis strains (#309, #311 and #573) isolated from Brazilian crude oils was optimized based on different carbon and nitrogen sources. The lowest surface tension values were obtained using sucrose containing media for the three isolates. Biosurfactants produced by each strain were characterized by Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ( 1 H NMR), and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). The chemical characterization showed that the three isolates produced very similar mixtures, containing C13-, C14- and C15-surfactin, although in different proportions. The structure of surfactins produced by the three strains was shown to be in good agreement with their surface-activities. The interfacial-activities of the biosurfactants were characterized and compared with chemical surfactants. Furthermore, the application of biosurfactants and chemical surfactants in oil recovery was evaluated. The results obtained showed that biosurfactants have better interfacial-activity and oil recovery efficiency than common chemical surfactants, thus being more attractive to be applied in Microbial Enhanced Oil Recovery.

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Metadata

Authors
Pereira, J. F. B., Gudiña, E. J., Costa, R., Vitorino, R., Teixeira, J. A., Coutinho, J. A. P., & Rodrigues, L. R.
Venue
Fuel
Publisher
Elsevier BV
Published
2013-09
Volume
111
Pages
259-268
ISSN
0016-2361 (ISSN-L)
OpenAlex
W2136848594
Cited by
378
References
6
FWCI
22.27
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. https://app.cochat.ai/papers/10.1016/j.fuel.2013.04.040
The paper
Pereira, J. F. B., Gudiña, E. J., Costa, R., Vitorino, R., Teixeira, J. A., Coutinho, J. A. P., & Rodrigues, L. R. (2013). Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. Fuel. https://doi.org/10.1016/j.fuel.2013.04.040

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.