Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes

Bäck, Karolina; Arnqvist, Hans J

doi:10.1016/j.ghir.2008.06.004

Cited by 40Adipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic DiseasesGrowth Hormone and Insulin-like Growth Factors

Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes

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Bäck, Karolina, Arnqvist, Hans J·2009·Growth Hormone & IGF Research

DOI 10.1016/j.ghir.2008.06.004

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Abstract

Mature adipocytes originate from fibroblast-like precursor cells, preadipocytes, which differentiate to obtain the characteristics of adipocytes. Our aim was to investigate how differentiation of human preadipocytes affects the distribution of insulin receptors (IR) and IGF-I receptors (IGF-IR) and other cell characteristics. Preadipocytes were differentiated using indomethacine, dexamethasone, isobutyl-methylxantine (IBMX) and high concentration of insulin. Gene expression was quantified by real-time RT-PCR in preadipocytes (PA), differentiated preadipocytes (dPA) and mature adipocytes (mAD). The amount of expressed receptor protein was analyzed using receptor specific ELISAs and Western blot. We also studied DNA synthesis with radiolabeled thymidine incorporation and glucose accumulation with radiolabeled glucose. Differentiation of PA increased gene expression of IR but not IGF-IR. GLUT4, growth hormone receptor (GHR) and adiponectin appeared or increased. In PA and dPA only IR-A was expressed whereas also IR-B was detected in mAD. By Western blot and ELISA, IR and IGF-IR were detected in PA, dPA and mAD. During differentiation the ratio of IR to IGF-IR increased severalfold. In PA both the IR and the IGF-IR was phosphorylated by their own ligand at 1 nM and in dPA the activation of both receptors was stimulated by IGF-I, but not insulin, at 1 nM. Accumulation of glucose in PA was increased by insulin at 10nM and by IGF-I at 1 nM and 10nM. DNA synthesis was increased by insulin and IGF-I at 10nM. In conclusion, both IR and IGF-IR are present in human preadipocytes and adipocytes. Differentiation is characterized by an increased IR/IGF-IR ratio.

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Besides lipids themselves, local levels of insulin, glucocorticoids, sex steroids, and bone morphogenetic proteins (BMPs) also directly regulate peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α (CEBPα), to facilitate preadipocyte differentiation.
“Besides lipids themselves, local levels of insulin, glucocorticoids, sex steroids, and bone morphogenetic proteins (BMPs) also directly regulate peroxisome proliferator‐activated receptor‐γ (PPARγ) and CCAAT/enhancer‐binding protein‐α (CEBPα), to facilitate preadipocyte differentiation.[ 29 ]”
From Pathogenesis of sarcopenia and the relationship with fat mass: descriptive review (2022) · cites this paper as [29]
Hyperlipidaemia, hyperinsulinaemia, and other metabolic signalling cues drive the creation and expansion of new lipid storage depots during fat redistribution.
“Thus, hyperlipidaemia, hyperinsulinaemia, and other metabolic signalling cues drive the creation and expansion of new lipid storage depots during fat redistribution.[ 29 ]”
From Pathogenesis of sarcopenia and the relationship with fat mass: descriptive review (2022) · cites this paper as [29]

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Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes
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Characterization of the insulin and insulin-like growth factor receptors and responsitivity of a fibroblast/adipocyte cell line before and after differentiation
Shimizu, M., Torti, F. M., & Roth, R. A. (1986) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(86)91246-5 · Cited by 51 · see citing works
Differential effects of IGF-I, IGF-II and insulin in human preadipocytes and adipocytes – Role of insulin and IGF-I receptors
Bäck, K., Brännmark, C., Strålfors, P., & Arnqvist, H. J. (2011) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2011.04.005 · Cited by 30 · see citing works
Self-citation
Insulin-like growth factor-I is an essential regulator of the differentiation of 3T3-L1 adipocytes.
Smith, P. J., Wise, L. S., Berkowitz, R., Wan, C. F., & Rubin, C. S. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)76555-7 · Cited by 511 · see citing works
P47 Differential effects of IGF-I, IGF-II and insulin in human preadipocytes and adipocytes – role of insulin and IGF-I receptors
Bäck, K., Brännmark, C., Strålfors, P., & Arnqvist, H. J. (2010) · Growth Hormone & IGF Research · doi:10.1016/s1096-6374(10)70147-7
Self-citation

Authors: Bäck, K., & Arnqvist, H. J.
Affiliations: Linköping University (SE)
Corresponding author: Bäck, Karolina
ORCID: Arnqvist, Hans J
Venue: Growth Hormone & IGF Research
Publisher: Elsevier BV
Published: 2009-04
Volume: 19
Issue: 2
Pages: 101-111
ISSN: 1096-6374 (ISSN-L)
DOI: 10.1016/j.ghir.2008.06.004
OpenAlex: W2033392856
Cited by: 40
References: 37
Citations by year: 2026: 2 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 4 · 2017: 1 · 2016: 4 · 2015: 2 · 2013: 4 · 2012: 3
FWCI: 2.48
Type: journal article
Language: en
Open access: green
urn.kb.se
Licence: other-oa
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Growth Hormone & IGF Research (publishedVersion)
PubMed (publishedVersion)
KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
Topics: Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords: insulin receptor, IGF-1 receptor, preadipocyte differentiation, adipogenesis, insulin-like growth factor I, insulin, GLUT4, growth hormone receptor, adiponectin, receptor phosphorylation, glucose uptake, DNA synthesis, gene expression
MeSH terms: Adult, Aged, Aged, 80 and over, Antigens, Differentiation, Cell Differentiation, Cells, Cultured, Female, Humans, Insulin, Insulin-Like Growth Factor I, Middle Aged, Phosphorylation, Receptor, Insulin, Receptor, IGF Type 1, Adipocytes, Adult Stem Cells

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Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes

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