The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection
Open accessAbstract
Mass sequencing of cDNA libraries from salivary glands of triatomines has resulted in the identification of many novel genes of unknown function. The aim of the present work was to develop a functional RNA interference (RNAi) technique for Rhodnius prolixus, which could be widely used for functional genomics studies in triatomine bugs. To this end, we investigated whether double-stranded RNA (dsRNA) can inhibit gene expression of R. prolixus salivary nitrophorin 2 (NP2) and what impact this might have on anticoagulant and apyrase activity in the saliva. dsRNA was introduced by two injections or by ingestion. RT-PCR of the salivary glands showed that injections of 15 μg of NP2 dsRNA in fourth-instar nymphs reduced gene expression by 75±14% and that feeding 1 μg/μL of NP2 dsRNA into second-instar nymphs (approx. 13 μg in total) reduced gene expression by 42±10%. Phenotype analysis showed that saliva of normal bugs prolonged plasma coagulation by about four-fold when compared to saliva of knockdown bugs. These results and the light color of the salivary gland content from some insects are consistent with the knockdown findings. The findings suggest that RNAi will prove a highly valuable functional genomics technique in triatomine bugs. The finding that feeding dsRNA can induce knockdown is novel for insects.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Hannon, G. J. (2002) · Nature · doi:10.1038/418244a · Cited by 3,538
Expression profiling reveals off-target gene regulation by RNAi
Jackson, A. L., Bartz, S. R., Schelter, J., Kobayashi, S. V., Burchard, J., Mao, M., Li, B., Cavet, G., & Linsley, P. S. (2003) · Nature Biotechnology · doi:10.1038/nbt831 · Cited by 2,370
Specific interference by ingested dsRNA
Timmons, L., & Fire, A. (1998) · Nature · doi:10.1038/27579 · Cited by 1,899
Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
Fraser, A. G., Kamath, R. S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M., & Ahringer, J. (2000) · Nature · doi:10.1038/35042517 · Cited by 1,765
Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi
Hammond, S. M., Boettcher, S., Caudy, A. A., Kobayashi, R., & Hannon, G. J. (2001) · Science · doi:10.1126/science.1064023 · Cited by 1,580
Kamath, R. S., Martinez-Campos, M., Zipperlen, P., Fraser, A. G., & Ahringer, J. (2000) · Genome Biology · doi:10.1186/gb-2000-2-1-research0002 · Cited by 1,295
Kennerdell, J. R., & Carthew, R. W. (1998) · Cell · doi:10.1016/s0092-8674(00)81725-0 · Cited by 1,132
Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
Gönczy, P., Echeverri, C., Oegema, K., Coulson, A., Jones, S. J. M., Copley, R. R., Duperon, J., Oegema, J., Brehm, M., Cassin, E., et al. (2000) · Nature · doi:10.1038/35042526 · Cited by 867
Cited by
RNAi-mediated crop protection against insects
Price, D. R. G., & Gatehouse, J. A. (2008) · Trends in Biotechnology · doi:10.1016/j.tibtech.2008.04.004 · Cited by 585
Silencing of Aphid Genes by dsRNA Feeding from Plants
Pitino, M., Coleman, A. D., Maffei, M. E., Ridout, C. J., & Hogenhout, S. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025709 · Cited by 414
Delivery of dsRNA for RNAi in insects: an overview and future directions
Yu, N., Christiaens, O., Liu, J., Niu, J., Cappelle, K., Caccia, S., Huvenne, H., & Smagghe, G. (2013) · Insect Science · doi:10.1111/j.1744-7917.2012.01534.x · Cited by 375
Ibrahim, A. B., Monteiro, T. R., Cabral, G. B., & Aragão, F. J. L. (2017) · Transgenic Research · doi:10.1007/s11248-017-0035-0 · Cited by 66
Metadata
- Authors
- Araujo, R. N., Santos, A., Pinto, F. S., Gontijo, N. F., Lehane, M. J., & Pereira, M. H.
- Venue
- Insect Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2006-09
- Volume
- 36
- Issue
- 9
- Pages
- 683-693
- ISSN
- 0965-1748 (ISSN-L)
- OpenAlex
- W2001256177
- Cited by
- 276
- References
- 27
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection. https://app.cochat.ai/papers/10.1016/j.ibmb.2006.05.012
- The paper
- Araujo, R. N., Santos, A., Pinto, F. S., Gontijo, N. F., Lehane, M. J., & Pereira, M. H. (2006). RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection. Insect Biochemistry and Molecular Biology. https://doi.org/10.1016/j.ibmb.2006.05.012
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
