The DOI matches its Crossref record; 72 of the 72 references it cites were found in a registry or an index.
Delivery of dsRNA for RNAi in insects: an overview and future directions
Abstract
Abstract RNA interference (RNAi) refers to the process of exogenous double‐stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi‐based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.
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Cites
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“The plants selected in these studies were transgenic models such as rice Oryza sativa (Zha et al., 2011), tobacco Nicotiana tabacum (Mao et al., 2007), Arabidopsis thaliana, and cotton Gossypium hirsutum (Mao et al., 2011).”
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Cited by
Mechanisms, Applications, and Challenges of Insect RNA Interference
Zhu, K. Y., & Palli, S. R. (2020) · Annual Review of Entomology · doi:10.1146/annurev-ento-011019-025224 · Cited by 709
Insect immunology and hematopoiesis
Hillyer, J. F. (2016) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2015.12.006 · Cited by 561
Silencing of Aphid Genes by dsRNA Feeding from Plants
Pitino, M., Coleman, A. D., Maffei, M. E., Ridout, C. J., & Hogenhout, S. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025709 · Cited by 414
Next-generation insect-resistant plants: RNAi-mediated crop protection
Zhang, J., Khan, S. A., Heckel, D. G., & Bock, R. (2017) · Trends in Biotechnology · doi:10.1016/j.tibtech.2017.04.009 · Cited by 348
Yan, S., Ren, B., & Shen, J. (2021) · Insect Science · doi:10.1111/1744-7917.12822 · Cited by 303
Metadata
- Authors
- Yu, N., Christiaens, O., Liu, J., Niu, J., Cappelle, K., Caccia, S., Huvenne, H., & Smagghe, G.
- Venue
- Insect Science
- Publisher
- Wiley
- Published
- 2012-07-05
- Volume
- 20
- Issue
- 1
- Pages
- 4-14
- ISSN
- 1005-295X (ISSN-L)
- 1672-9609
- 1744-7917
- OpenAlex
- W2100394661
- Cited by
- 375
- References
- 72
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yu, N., Christiaens, O., Liu, J., Niu, J., Cappelle, K., Caccia, S., Huvenne, H., & Smagghe, G. (2013). Delivery of dsRNA for RNAi in insects: an overview and future directions. Insect Science. https://doi.org/10.1111/j.1744-7917.2012.01534.x
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