RNAi-mediated resistance to whitefly (Bemisia tabaci) in genetically engineered lettuce (Lactuca sativa)
Abstract
RNA interference (RNAi)-based transgenic technologies have evolved as potent biochemical tools for silencing specific genes of plant pathogens and pests. The approach has been demonstrated to be useful in silencing genes in insect species. Here, we report on the successful construction of RNAi-based plasmid containing an interfering cassette designed to generate dsRNAs that target a novel v-ATPase transcript in whitefly (Bemisia tabaci), an important agricultural pest in tropical and sub-tropical regions. The presence of the transgene was confirmed in T 0 and T 1 generations of transgenic lettuce lines, segregating in a Mendelian fashion. Seven lines were infested with whiteflies and monitored over a period of 32 days. Analysis of mortality showed that within five days of feeding, insects on transgenic plants showed a mortality rate of 83.8-98.1%. In addition, a reduced number of eggs (95 fold less) was observed in flies feeding on transgenic lettuce plants than insects on control lines. Quantitative reverse transcription PCR showed decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. This technology is a foundation for the production of whitefly-resistant commercial crops, improving agricultural sustainability and food security, reducing the use of more environmentally aggressive methods of pest control.
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RNAi can target hemipteran pests, including phloem-feeders, that have not yet been targeted by Bt proteins.
“What makes the technology interesting is the fact that one can also target hemipteran pests (including phloem-feeders) that have not yet been targeted by Bt proteins (Baum and Roberts, 2014;Ibrahim et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Ibrahim et al., 2017
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429 · see citing works
Pahlich, E., & Gerlitz, C. (1980) · Phytochemistry · doi:10.1016/0031-9422(80)85004-7 · Cited by 10,682 · see citing works
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837 · see citing works
Control of coleopteran insect pests through RNA interference
Baum, J. A., Bogaert, T., Clinton, W., Heck, G. R., Feldmann, P., Ilagan, O., Johnson, S., Plaetinck, G., Munyikwa, T., Pleau, M., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1359 · Cited by 1,789 · see citing works
Vacuolar ATPases: rotary proton pumps in physiology and pathophysiology
Forgac, M. D. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2272 · Cited by 1,626 · see citing works
Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358 · cited 2× · see citing works
“…in experiments showing silencing of genes in western corn rootworm (Diabrotica virgifera) (Baum et al. 2007), cotton bollworm (Helicoverpa armigera) (Mao et al. 2007), brown plant hopper (Nilaparvara lugens) (Zha et al. 2011) and tobacco hornworm (Manduca sexta) (Kumar et al. 2012) among others.”
BackgroundRevision of Begomovirus taxonomy based on pairwise sequence comparisons
Brown, J. K., Júnior, F. M. Z., Navas‐Castillo, J., et al. (2015) · Archives of Virology · doi:10.1007/s00705-015-2398-y · Cited by 824 · see citing works
MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasis
Pant, B. D., Buhtz, A., Kehr, J., et al. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03363.x · Cited by 799 · see citing works
The V-type H+ ATPase: molecular structure and function,physiological roles and regulation
Beyenbach, K. W., & Wieczorek, H. (2006) · Journal of Experimental Biology · doi:10.1242/jeb.02014 · Cited by 630 · see citing works
RNAi-mediated crop protection against insects
Price, D. R. G., & Gatehouse, J. A. (2008) · Trends in biotechnology · doi:10.1016/j.tibtech.2008.04.004 · Cited by 585 · see citing works
Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium
Tomoyasu, Y., Miller, S., Tomita, S., Schoppmeier, M., Großmann, D., & Bucher, G. (2008) · Genome biology · doi:10.1186/gb-2008-9-1-r10 · Cited by 578 · see citing works
A Systemic Small RNA Signaling System in Plants
Yoo, B.-C., Kragler, F., Varkonyi‐Gasic, E., Haywood, V., Archer-Evans, S., Lee, Y. M., Lough, T. J., & Lucas, W. J. (2004) · The Plant Cell · doi:10.1105/tpc.104.023614 · Cited by 533 · see citing works
Towards the elements of successful insect RNAi
Scott, J. G., Michel, K., Bartholomay, L. C., Siegfried, B. D., Hunter, W. B., Smagghe, G. J., Zhu, K. Y., & Douglas, A. E. (2013) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2013.08.014 · Cited by 490 · see citing works
Biological control of Bemisia tabaci using predators and parasitoids
Gerling, D., Alomar, Ò., & Arnó, J. (2001) · Crop Protection · doi:10.1016/s0261-2194(01)00111-9 · Cited by 397 · see citing works
Delivery of dsRNA for RNAi in insects: an overview and future directions
Yu, N., Christiaens, O., Liu, J., et al. (2012) · Insect Science · doi:10.1111/j.1744-7917.2012.01534.x · Cited by 376 · see citing works
Identification and characterization of small RNAs from the phloem of Brassica napus
Buhtz, A., Springer, F., Chappell, L., Baulcombe, D. C., & Kehr, J. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03368.x · Cited by 372 · see citing works
Gordon, K. H. J., & Waterhouse, P. M. (2007) · Nature Biotechnology · doi:10.1038/nbt1107-1231 · Cited by 361 · see citing works
Biological control of Bemisia tabaci with fungi
de Faria, M. R., & Wraight, S. P. (2001) · Crop Protection · doi:10.1016/s0261-2194(01)00110-7 · Cited by 357 · see citing works
Whitefly-Transmitted Geminiviruses and Associated Disorders in the Americas and the Caribbean Basin
Brown, J. K. (1992) · Plant Disease · doi:10.1094/pd-76-0220 · Cited by 351 · see citing works
Bonfim, K., de Faria, J. C., Nogueira, E. O. P. L., Mendes, É. A., & Aragão, F. J. L. (2007) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-20-6-0717 · Cited by 338 · see citing works
Self-citationTurner, C. T., Davy, M. W., MacDiarmid, R. M., Plummer, K. M., Birch, N. P., & Newcomb, R. D. (2006) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2006.00656.x · Cited by 336 · see citing works
Zha, W., Peng, X., Chen, R., Du, B., Zhu, L., & He, G. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020504 · Cited by 328 · see citing works
Long distance transport and movement of RNA through the phloem
Kehr, J., & Buhtz, A. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm176 · Cited by 291 · see citing works
Araujo, R., Santos, A., Pinto, F. S., Gontijo, N. F., Lehane, M., & Pereira, M. H. (2006) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2006.05.012 · Cited by 275 · see citing works
RNA interference for the control of whiteflies (Bemisia tabaci) by oral route
Upadhyay, S. K., Chandrashekar, K., Thakur, N., Verma, P. C., Borgio, J. F., Singh, P. K., & Tuli, R. (2011) · Journal of Biosciences · doi:10.1007/s12038-011-9009-1 · Cited by 249 · see citing works
Feasibility, limitation and possible solutions of RNAi‐based technology for insect pest control
Zhang, H., Li, H., & Miao, X. (2012) · Insect Science · doi:10.1111/j.1744-7917.2012.01513.x · Cited by 217 · see citing works
Biotype and insecticide resistance status of the whitefly Bemisia tabaci from China
Wang, Z., Yan, H., Yang, Y., et al. (2010) · Pest Management Science · doi:10.1002/ps.2023 · Cited by 216 · see citing works
Thakur, N., Upadhyay, S. K., Verma, P. C., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0087235 · Cited by 189 · see citing works
Tinoco, M. L. P., Dias, B. B. A., D’Allastta, R. C., et al. (2010) · BMC Biology · doi:10.1186/1741-7007-8-27 · Cited by 173 · see citing works
Kumar, M., Gupta, G. P., & Rajam, M. V. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2008.12.005 · Cited by 169 · see citing works
Kumar, P., V, Pandit, S. S., & Baldwin, I. T. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031347 · Cited by 152 · see citing works
Host plants and natural enemies of Bemisia tabaci (Hemiptera: Aleyrodidae) in China
Li, S., Xia, X., Ahmed, M. Z., et al. (2011) · Insect Science · doi:10.1111/j.1744-7917.2010.01395.x · Cited by 131 · see citing works
Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius)
Ghanim, M., Kontsedalov, S., & Czosnek, H. H. (2007) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2007.04.006 · Cited by 130 · see citing works
Davies, S. A., Goodwin, S. F., Kelly, D. C., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.48.30677 · Cited by 128 · see citing works
Host‐induced gene silencing in the necrotrophic fungal pathogen Sclerotinia sclerotiorum
Andrade, C. M., Tinoco, M. L. P., Rieth, A., et al. (2015) · Plant Pathology · doi:10.1111/ppa.12447 · Cited by 111 · see citing works
Luan, J., Ghanim, M., Liu, S., & Czosnek, H. H. (2013) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2013.05.012 · Cited by 111 · see citing works
Malik, H. J., Raza, A., Amin, I. U., Scheffler, J., Scheffler, B. E., Brown, J. K., & Mansoor, S. (2016) · Scientific Reports · doi:10.1038/srep38469 · Cited by 99 · see citing works
Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci
Chen, X., Li, L., Hu, Q., et al. (2015) · BMC Biotechnology · doi:10.1186/s12896-015-0170-8 · Cited by 91 · see citing works
Cuthbertson, A. G. S., & Walters, K. F. A. (2005) · Mycopathologia · doi:10.1007/s11046-005-0122-2 · Cited by 85 · see citing works
Dias, B. B. A., Cunha, W. G., Morais, L. S., et al. (2006) · Plant Pathology · doi:10.1111/j.1365-3059.2006.01342.x · Cited by 74 · see citing works
Raza, A., Malik, H. J., Shafiq, M., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0153883 · Cited by 74 · see citing works
Karatolos, N., Denholm, I., Williamson, M. S., et al. (2010) · Pest Management Science · doi:10.1002/ps.2014 · Cited by 68 · see citing works
Molecular Genetic Analysis of V-ATPase Function in Drosophila Melanogaster
Dow, J. A. T., Davies, S. A., Guo, Y., et al. (1997) · Journal of Experimental Biology · doi:10.1242/jeb.200.2.237 · Cited by 68 · see citing works
Cuthbertson, A. G. S., Walters, K. F. A., & Northing, P. (2005) · Mycopathologia · doi:10.1007/s11046-004-3127-3 · Cited by 65 · see citing works
Shah, M. M. R., & Liu, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0077368 · Cited by 52 · see citing works
Insect gut nucleases: a challenge for RNA interference mediated insect control strategies
Katoch, R., & Thakur, N. (2012) · Cited by 52 · see citing works
Golden Mosaic of Common Beans in Brazil: Management with a Transgenic Approach
de Faria, J. C., Aragão, F. J. L., SOUZA, T. L. P. O., et al. (2016) · APSnet Feature Articles · doi:10.1094/apsfeature-2016-10 · Cited by 43 · see citing works
Self-citationAsokan, R., Rebijith, K. B., Roopa, H. K., & Kumar, N. K. K. (2015) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-014-1437-6 · Cited by 24 · see citing works
RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants
Ibrahim, A. B., & Aragão, F. J. L. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2453-0_5 · Cited by 24 · see citing works
Self-citationCathrin, P. B., & Ghanim, M. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-411584-2.00004-4 · Cited by 22 · see citing works
Rivnay, T., & Gerling, D. (1987) · BioControl · doi:10.1007/bf02373515 · Cited by 21 · see citing works
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Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
- Authors
- Ibrahim, A. B., Monteiro, T. R., Cabral, G. B., & Aragão, F. J. L.
- Affiliations
- Ahmadu Bello University (NG)
- Universidade de Brasília (BR)
- Embrapa (BR)
- Corresponding author
- Aragão, Francisco J. L.
- Venue
- Transgenic Research
- Published
- 2017-07-15
- Volume
- 26
- Issue
- 5
- Pages
- 613-624
- ISSN
- 0962-8819 (ISSN-L)
- 1573-9368
- OpenAlex
- W2735687824
- Cited by
- 66
- References
- 52
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 7 · 2023: 10 · 2022: 11 · 2021: 10 · 2020: 5 · 2019: 4 · 2018: 5
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Transgenic Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- RNA interference, Bemisia tabaci, whitefly resistance, transgenic lettuce, lettuce, V-ATPase, insect pest control, transgene segregation, insect mortality
- MeSH terms
- Animals, Genetic Engineering, Hemiptera, Pest Control, RNA, Double-Stranded, Lactuca, Crops, Agricultural, Vacuolar Proton-Translocating ATPases, Plants, Genetically Modified, Disease Resistance
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