Cited by 177biodegradable polymer synthesis and propertiesAlgal biology and biofuel productionBiopolymer Synthesis and Applications

Influence of Nitrogen on Growth, Biomass Composition, Production, and Properties of Polyhydroxyalkanoates (PHAs) by Microalgae

Costa, Samantha Serra, Miranda, Andréa Lobo, Andrade, Bianca Bomfim, Assis, Denilson de Jesus, Souza, Carolina Oliveira, de Morais, Michele Greque, Costa, Jorge Alberto Vieira, Druzian, Janice Izabel·2018·International Journal of Biological Macromolecules

Abstract

This study sought to evaluate influence of nitrogen availability on cell growth, biomass composition, production, and the properties of polyhydroxyalkanoates during cultivation of microalgae Chlorella minutissima, Synechococcus subsalsus, and Spirulina sp. LEB-18. The cellular growth of microalgae reduced with the use of limited nitrogen medium, demonstrating that nitrogen deficiency interferes with the metabolism of microorganisms and the production of biomass. The biochemical composition of microalgae was also altered, which was most notable in the degradation of proteins and chlorophylls and the accumulation of carbonaceous storage molecules such as lipids and polyhydroxyalkanoates. Chlorella minutissima did not produce these polymers even in a nitrogen deficient environment. The largest accumulations of the polyhydroxyalkanoates occurred after a 15 days culture, with a concentration of 16% (dry cell weight) produced by the Synechococcus subsalsus strain and 12% by Spirulina sp. LEB-18. Polyhydroxyalkanoates produced by Synechococcus subsalsus and Spirulina sp. LEB-18 presented different thermal and physical properties, indicating the influence of producing strain on polyhydroxyalkanoates properties. The polymers obtained consisted of long chain monomers with 14 to 18 carbon atoms. This composition is novel, as it has not previously been found in PHAs obtained from Synechococcus subsalsus and Spirulina sp. LEB-18.

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Crossref · 10.1016/j.ijbiomac.2018.05.064

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  • Coelho et al. and Costa et al. studied PHA production from Spirulina sp., showing yields of 30.7% PHA and 12.0% PHA (w/w dry biomass), respectively.

    “For instance, Coelho et al. [197] and Costa et al. [198] have studied PHA production from Spirulina sp., showing yields of 30.7% PHA (w/w dry biomass) and 12.0% PHA (w/w dry biomass), respectively.”

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Authors
Costa, S. S., Miranda, A. L., Andrade, B. B., Assis, D. D. J., Souza, C. O., de Morais, M. G., Costa, J. A. V., & Druzian, J. I.
Affiliations
  • Universidade Federal da Bahia (BR)
  • Universidade Federal do Rio Grande (BR)
Corresponding author
Costa, Samantha Serra
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2018-09
Volume
116
Pages
552-562
ISSN
0141-8130 (ISSN-L)
OpenAlex
W2800907662
Cited by
177
References
45
Citations by year
2026: 21 · 2025: 23 · 2024: 33 · 2023: 24 · 2022: 32 · 2021: 19 · 2020: 13 · 2019: 9 · 2018: 3
FWCI
3.56
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Science Technology and Innovation (01200.005005/2014-49)
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
Keywords
polyhydroxyalkanoates, nitrogen limitation, microalgae, Chlorella minutissima, microalgae-bacteria co-culture, biomass composition, biopolymer production
MeSH terms
Chlorella, Nitrogen, Biomass, Synechococcus, Spirulina, Polyhydroxyalkanoates, Microalgae

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