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Influence of nitrogen on growth, biomass composition, production, and properties of polyhydroxyalkanoates (PHAs) by microalgae
Abstract
This study sought to evaluate influence of nitrogen availability on cell growth, biomass composition, production, and the properties of polyhydroxyalkanoates during cultivation of microalgae Chlorella minutissima, Synechococcus subsalsus, and Spirulina sp. LEB-18. The cellular growth of microalgae reduced with the use of limited nitrogen medium, demonstrating that nitrogen deficiency interferes with the metabolism of microorganisms and the production of biomass. The biochemical composition of microalgae was also altered, which was most notable in the degradation of proteins and chlorophylls and the accumulation of carbonaceous storage molecules such as lipids and polyhydroxyalkanoates. Chlorella minutissima did not produce these polymers even in a nitrogen deficient environment. The largest accumulations of the polyhydroxyalkanoates occurred after a 15 days culture, with a concentration of 16% (dry cell weight) produced by the Synechococcus subsalsus strain and 12% by Spirulina sp. LEB-18. Polyhydroxyalkanoates produced by Synechococcus subsalsus and Spirulina sp. LEB-18 presented different thermal and physical properties, indicating the influence of producing strain on polyhydroxyalkanoates properties. The polymers obtained consisted of long chain monomers with 14 to 18 carbon atoms. This composition is novel, as it has not previously been found in PHAs obtained from Synechococcus subsalsus and Spirulina sp. LEB-18.
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“For instance, Coelho et al. [197] and Costa et al. [198] have studied PHA production from Spirulina sp., showing yields of 30.7% PHA (w/w dry biomass) and 12.0% PHA (w/w dry biomass), respectively.”
From Emerging Applications of Chlorella sp. and Spirulina (Arthrospira) sp. (2023) · cites this paper as [198]
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Metadata
- Authors
- Costa, S. S., Miranda, A. L., Andrade, B. B., Assis, D. D. J., Souza, C. O., de Morais, M. G., Costa, J. A. V., & Druzian, J. I.
- Affiliations
- Universidade Federal da Bahia (BR)
- Universidade Federal do Rio Grande (BR)
- Corresponding author
- Costa, Samantha Serra
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 116
- Pages
- 552-562
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2800907662
- Cited by
- 177
- References
- 45
- Citations by year
- 2026: 21 · 2025: 23 · 2024: 33 · 2023: 24 · 2022: 32 · 2021: 19 · 2020: 13 · 2019: 9 · 2018: 3
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Science Technology and Innovation (01200.005005/2014-49)
- Topics
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
- Keywords
- polyhydroxyalkanoates, nitrogen limitation, microalgae, Chlorella minutissima, microalgae-bacteria co-culture, biomass composition, biopolymer production
- MeSH terms
- Chlorella, Nitrogen, Biomass, Synechococcus, Spirulina, Polyhydroxyalkanoates, Microalgae
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