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Cited by 36Burkholderia infections and melioidosisInfections and bacterial resistanceBacterial biofilms and quorum sensing

IL-8 released from human lung epithelial cells induced by cystic fibrosis pathogens Burkholderia cepacia complex affects the growth and intracellular survival of bacteria

Kaza, Seshu K., McClean, Siobhán, Callaghan, Máire·2011·International Journal of Medical Microbiology
DOI 10.1016/j.ijmm.2010.06.005
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Abstract

Burkholderia cepacia complex (Bcc) is a group of Gram-negative pulmonary pathogens associated with life-threatening infections in patients with cystic fibrosis (CF). The airway epithelium plays a crucial role in the initiation and modulation of inflammatory responses to these pathogens. Interleukin (IL)-8 released from epithelial cells is a potent chemoattractant for neutrophils. The aims of this study were to compare the IL-8 response to Bcc infection in different epithelial cell types and to investigate the impact of IL-8 on Bcc growth and intracellular survival. To compare epithelial cell IL-8 responses, 4 human epithelial cell lines were used in the study; A549 cells, an alveolar epithelial cell line, Calu-3 cells, a sub-bronchial epithelial cell line, 16HBE14o- cells, and CFBE41o- cells, which are CFTR-positive and CFTR-negative bronchial epithelial cell lines, respectively. Two B. multivorans and 2 B. cenocepacia strains all induced a significant IL-8 response by 12 h and further increased in all cell lines at 24 h. Furthermore, the levels of IL-8 from Calu-3 and A549 cells were approximately 3 times that of 16HBE14o- or CFBE41o- cells. In 2 of the cell lines examined (16HBE14o- and CFBE41o-), B. cenocepacia LMG 16656 (J2315), an epidemic strain, induced greater levels of IL-8 (P<0.01) compared to other Bcc strains tested. The CFTR-positive and -negative cell lines secreted similar levels of IL-8 indicating a CFTR-independent induction of IL-8. However, the CFTR-negative cells did secrete constitutive levels of IL-8 greater than that of CFTR-positive cells. An investigation of the effect of IL-8 on Bcc extracellular and intracellular growth found that at low concentrations (<10 ng/ml) of recombinant human (rh) IL-8, the growth of B. cenocepacia LMG 16656 and B. multivorans LMG 13010 was enhanced, whereas at higher concentrations (10 ng/ml), growth of both strains was significantly reduced. Growth of both non-CF Bcc strains remained unchanged in the presence of rhIL-8. In contrast to extracellular growth, higher concentrations (10ng/ml) of rhIL-8 enhance the intracellular growth and survival of both LMG 16656 and LMG 13010 in 16HBE14o- and CFBE41o- cell lines. Although LMG 13010 uptake by epithelial cells was higher than LMG 16656 (P<0.01), the intracellular growth of LMG 16656 is greater than LMG 13010 (P<0.05). These studies demonstrated that the type of epithelial cells encountered by Bcc strains determines the extent of the IL-8 responses triggered and that this cytokine in addition to its well-established proinflammatory properties can enhance both the extracellular and intracellular growth of Bcc strains.

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  • Growth promotion of bacteria is also seen with TNFα, interleukins 1, 6, and 8, and glucocorticoids.

    “as well as catecholamines, growth promotion is seen with TNFα, interleukins 1, 6, and 8, and glucocorticoids.”

    From The microbiome and critical illness (2016) · cites this paper as [124]

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Authors
Kaza, S. K., McClean, S., & Callaghan, M.
Affiliations
Institute of Technology Tallaght (IE)
Corresponding author
Callaghan, Máire
ORCID
McClean, Siobhán
Venue
International Journal of Medical Microbiology
Publisher
Elsevier BV
Published
2011-01
Volume
301
Issue
1
Pages
26-33
ISSN
1438-4221 (ISSN-L)
DOI
10.1016/j.ijmm.2010.06.005
OpenAlex
W2027248916
Cited by
36
References
55
Citations by year
2026: 1 · 2025: 1 · 2024: 3 · 2022: 4 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 5 · 2015: 7 · 2014: 4 · 2013: 1 · 2012: 1
FWCI
1.16
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • International Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Higher Education Authority
Topics
  • Health Sciences › Medicine › Epidemiology › Burkholderia infections and melioidosis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Infections and bacterial resistance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Burkholderia cepacia complex, interleukin-8, cystic fibrosis, lung epithelial cells, Burkholderia multivorans, Burkholderia cenocepacia, intracellular bacterial survival, bacterial growth, CFTR, epithelial cell lines, airway inflammation, host-pathogen interaction, neutrophil recruitment, bacterial virulence factors, virulence gene expression
MeSH terms
Cell Line, Cystic Fibrosis, Epithelial Cells, Humans, Lung, Interleukin-8, Respiratory Mucosa, Burkholderia cepacia complex, Microbial Viability

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