Cited by 110Immune Response and InflammationSepsis Diagnosis and TreatmentNeutrophil, Myeloperoxidase and Oxidative Mechanisms

Effects of cytokines and endotoxin on the intracellular growth of bacteria

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Kanangat, Siva, Meduri, G. Umberto, Tolley, Elizabeth A., Patterson, David R., Meduri, Christopher U., Pak, Chol, Griffin, John P., Bronze, Michael S. +1 more·1999·Infection and Immunity

Abstract

Patients with unresolving acute respiratory distress syndrome (ARDS) have persistently elevated levels of proinflammatory cytokines in the lungs and circulation and increased rates of bacterial infections. Phagocytic cells hyperactivated with lipopolysaccharide (LPS), which induces high levels of proinflammatory cytokines in monocytic cells, are inefficient in killing ingested bacteria despite having intact phagocytic activity. On the other hand, phagocytic cells that are activated with an analogue of LPS that does not induce the expression of proinflammatory cytokines effectively ingest and kill bacteria. We hypothesized that in the presence of high concentrations of proinflammatory cytokines, bacteria may adapt and utilize cytokines to their growth advantage. To test our hypothesis, we primed a human monocytic cell line (U937) with escalating concentrations of the proinflammatory cytokines tumor necrosis factor alpha, interleukin-1beta (IL-1beta), and IL-6 and with LPS. These cells were then exposed to fresh isolates of three common nosocomial pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, and an Acinetobacter sp. In human monocytes primed with lower concentrations of proinflammatory cytokines (10 to 250 pg) or LPS (1 and 10 ng), intracellular bacterial growth decreased. However, when human monocytes were primed with higher concentrations of proinflammatory cytokines (1 to 10 ng) or LPS (1 to 10 micrograms), intracellular growth of the tested bacteria increased significantly (P <0.0001). These results were reproduced with peripheral blood monocytes obtained from normal healthy volunteers. The specificity of the cytokine activity was demonstrated by neutralizing the cytokines with specific antibodies. Our findings provide a possible mechanism to explain the frequent development of bacterial infections in patients with an intense and protracted inflammatory response.

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Crossref · 10.1128/iai.67.6.2834-2840.1999

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  • Growth promotion of bacteria is also seen with TNFα, interleukins 1, 6, and 8, and glucocorticoids.

    “as well as catecholamines, growth promotion is seen with TNFα, interleukins 1, 6, and 8, and glucocorticoids.”

    From The microbiome and critical illness (2016) · cites this paper as [24]

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Authors
Kanangat, S., Meduri, G. U., Tolley, E. A., Patterson, D. R., Meduri, C. U., Pak, C., Griffin, J. P., Bronze, M. S., & Schaberg, D. R.
Affiliations
  • <!--label omitted: 1-->Department of Medicine, Pulmonary and Critical Care Division,1
  • <!--label omitted: 2-->Department of Preventive Medicine, Division of Biostatistics and Epidemiology,2 and
  • <!--label omitted: 3-->Division of Infectious Diseases,3 University of Tennessee—Memphis, Memphis, Tennessee 38163
Venue
Infection and Immunity
Published
1999-06
Volume
67
Issue
6
Pages
2834-2840
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W1955721074
Cited by
110
References
20
Citations by year
2026: 2 · 2025: 4 · 2024: 4 · 2023: 5 · 2022: 9 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 8 · 2014: 4 · 2013: 1
FWCI
2.05
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Epidemiology › Sepsis Diagnosis and Treatment
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
intracellular bacterial growth, inflammatory cytokines, lipopolysaccharide, endotoxin, acute respiratory distress syndrome, nosocomial infections, monocytes, U937 cells, TNF-α, IL-1β, interleukin-6, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter, phagocyte activation
MeSH terms
Acinetobacter, Cells, Cultured, Humans, Interleukin-1, Intracellular Fluid, Lipopolysaccharides, Monocytes, Pseudomonas aeruginosa, RNA, Messenger, Staphylococcus aureus, Tumor Necrosis Factor-alpha, Interleukin-6, Gene Expression, Cytokines, Interleukin-10, U937 Cells

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