Dose-dependent food allergy induction against ovalbumin under acid-suppression: A murine food allergy model
Open accessAbstract
Background Animal models are essential for analyzing the allergenic potential of food proteins and for investigating mechanisms underlying food allergy. Based on previous studies revealing acid-suppression medication as risk factor for food allergy induction, we aimed to establish a mouse model mimicking the natural route of sensitization in patients.Methods The effect of acid-suppressing medication on murine gastric pH was assessed by intragastric pH measurements after two injections of a proton pump inhibitor (PPI). To investigate dose-dependency, mice were fed different concentrations of ovalbumin (OVA; 0.2, 0.5, 1.0, 2.5 or 5.0mg) either with or without anti-ulcer medication. Additionally, different routes of exposure (i.p. vs. oral) were compared in a second immunization experiment. Sera were screened for OVA-specific antibody titers (IgG1, IgG2a and IgE) in ELISA and RBL assay. Clinical reactivity was evaluated by measuring rectal temperature after oral challenge and by type I skin tests.Results Two intravenous injections of PPI significantly elevated the gastric pH from 2.97 to 5.3. Only oral immunization with 0.2mg OVA under anti-acid medication rendered elevated IgG1, IgG2a and IgE titers compared to all other concentrations. Protein feeding alone altered antibody titers only marginally. Even though also i.p. immunizations induced high levels of specific IgE, only oral immunizations under anti-acids induced anaphylactic reactions evidenced by a significant decrease of body temperature.Conclusion Only low-dosage ovalbumin feedings under anti-acid medication resulted in IgE mediated food allergy. Based on this knowledge we have established a suitable food allergy model for further investigations of food adverse reactions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Encapsulation of the food allergen OVA, which is degraded under physiological conditions during GI transit, remained stable when entrapped within PLGA NPs.
“In this study, encapsulation of the food allergen OVA, which was found to be degraded under physiological conditions during the GI transit [77], remained stable when entrapped within PLGA NPs.”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [77]
NA is highly stable under gastric and intestinal proteolytic conditions, making it an ideal targeting molecule for oral delivery.
“In addition, NA was revealed to be extremely stable under gastric and intestinal proteolytic conditions and, thus, represents an ideal targeting molecule for the oral route [19,36].”
From Characterization of Vibrio cholerae neuraminidase as an immunomodulator for novel formulation of oral allergy immunotherapy (2018) · cites this paper as [19]
Sample size calculation was based on previous own data with a power calculation based on two-sided, two-sample t-test.
“Sample size calculation was based on previous own data [19] with a power calculation based on two-sided, two-sample t-test.”
From Characterization of Vibrio cholerae neuraminidase as an immunomodulator for novel formulation of oral allergy immunotherapy (2018) · cites this paper as [19]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311 · see citing works
Of Mice and Not Men: Differences between Mouse and Human Immunology
Mestas, J., & Hughes, C. C. W. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.172.5.2731 · Cited by 3,822 · see citing works
Sampson, H. A. (2004) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2004.03.014 · Cited by 1,344 · see citing works
Barsumian, E. L., Isersky, C., Petrino, M. G., & Siraganian, R. P. (1981) · European Journal of Immunology · doi:10.1002/eji.1830110410 · Cited by 575 · see citing works
Rising prevalence of allergy to peanut in children: Data from 2 sequential cohorts
Grundy, J. D., Matthews, S., Bateman, B., Dean, T., & Arshad, S. H. (2002) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2002.128802 · Cited by 559 · see citing works
Brewer, J. M., Conacher, M., Hunter, C. A., Mohrs, M., Brombacher, F., & Alexander, J. K. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.12.6448 · Cited by 442 · see citing works
Fu, T.-J., Abbott, U. R., & Hatzos, C. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020599h · Cited by 403 · see citing works
Fu, T., Abbott, U. R., & Hatzos, C. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030375e · Cited by 384 · see citing works
Pathways of anaphylaxis in the mouse
Strait, R. T., Morris, S. C., Yang, M., Qu, X.-W., & Finkelman, F. D. (2002) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2002.123302 · Cited by 312 · see citing works
Untersmayr, E. (2003) · Journal of Allergy and Clinical Immunology · doi:10.1016/s0091-6749(03)01719-6 · Cited by 240 · see citing works
Self-citationAnti‐ulcer drugs promote IgE formation toward dietary antigens in adult patients
Untersmayr, E., Bakos, N., Pali‐Schöll, I., Kundi, M., Roth‐Walter, F., Szalai, K., Riemer, A. B., Ankersmit, H. J., Scheiner, O., Boltz‐Nitulescu, G., et al. (2005) · The FASEB Journal · doi:10.1096/fj.04-3170fje · Cited by 217 · see citing works
Self-citationMolecular mechanisms of anaphylaxis: Lessons from studies with murine models
Finkelman, F. D., Rothenberg, M. E., Brandt, E. B., Morris, S. C., & Strait, R. T. (2005) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2004.12.1125 · Cited by 211 · see citing works
Snider, D. P., Marshall, J. S., Perdue, M. H., et al. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.153.2.647 · Cited by 193 · see citing works
Pali‐Schöll, I., Untersmayr, E., Bakos, N., et al. (2005) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/81.1.154 · Cited by 152 · see citing works
Self-citationWill genetically modified foods be allergenic?
Taylor, S. L., & Hefle, S. L. (2001) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2001.114241 · Cited by 142 · see citing works
A novel model of sensitization and oral tolerance to peanut protein
Strid, J., Thomson, M., Hourihane, J. O., Kimber, I., & Ströbel, S. (2004) · Immunology · doi:10.1111/j.1365-2567.2004.01989.x · Cited by 124 · see citing works
Digestibility of peanut and hazelnut allergens investigated by a simple in vitro procedure
Vieths, S., Reindl, J., Müller, U., Hoffmann, A., & Haustein, D. (1999) · European Food Research and Technology · doi:10.1007/s002170050513 · Cited by 94 · see citing works
The effect of gastric digestion on food allergy
Untersmayr, E., & Jensen‐Jarolim, E. (2006) · Current Opinion in Allergy and Clinical Immunology · doi:10.1097/01.all.0000225163.06016.93 · Cited by 76 · see citing works
Self-citationHelm, R. M. (2002) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2002.tb04139.x · Cited by 74 · see citing works
A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice
SALDANHA, J. C. S., Gargiulo, D., Silva, S. S., et al. (2004) · Brazilian Journal of Medical and Biological Research · doi:10.1590/s0100-879x2004000600005 · Cited by 69 · see citing works
Pali‐Schöll, I., Ackermann, U., Özdemir, C., Blümer, N., Dicke, T., Sel, S., Sel, S., Wegmann, M., Szalai, K., Knittelfelder, R., et al. (2007) · The FASEB Journal · doi:10.1096/fj.06-7223com · Cited by 69 · see citing works
Self-citationEpitope characterization of ovalbumin in BALB/c mice using different entry routes
Mine, Y., & Yang, M. (2006) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2006.12.003 · Cited by 62 · see citing works
Hoffmann, A., Jamin, A., Foetisch, K., May, S., Aulepp, H., Haustein, D., & Vieths, S. (1999) · Allergy · doi:10.1034/j.1398-9995.1999.00917.x · Cited by 60 · see citing works
Elsayed, S., Hammer, A. S., Kalvenes, M. B., et al. (1986) · PubMed · Cited by 59 · see citing works
Animal models of food allergy: Opportunities and barriers
McClain, S., & Bannon, G. A. (2006) · Current Allergy and Asthma Reports · doi:10.1007/s11882-006-0052-1 · Cited by 56 · see citing works
Nonmurine animal models of food allergy.
Helm, R. M., Ermel, R. W., & Frick, O. L. (2003) · Environmental Health Perspectives · doi:10.1289/ehp.5705 · Cited by 47 · see citing works
Antigenic and Allergenic Determinants of Ovalbumin
Elsayed, S., Hammer, A. S. E., Kalvenes, M. B., et al. (2009) · International Archives of Allergy and Applied Immunology · doi:10.1159/000233952 · Cited by 41 · see citing works
Faquim-Mauro, E. L., & de Macedo, M. S. (2000) · International Immunology · doi:10.1093/intimm/12.12.1733 · Cited by 40 · see citing works
Ito, K., Inagaki‐Ohara, K., Murosaki, S., et al. (1997) · European Journal of Immunology · doi:10.1002/eji.1830271243 · Cited by 39 · see citing works
Characteristics of antibody responses induced in mice by protein allergens
Hilton, J., Dearman, R. J., Sattar, N., Basketter, D. A., & Kimber, I. (1997) · Food and Chemical Toxicology · doi:10.1016/s0278-6915(97)00119-1 · Cited by 38 · see citing works
Dearman, R. J., Caddick, H. T., Stone, S., et al. (2002) · Food and Chemical Toxicology · doi:10.1016/s0278-6915(01)00132-6 · Cited by 38 · see citing works
The impact of aluminium in acid‐suppressing drugs on the immune response of BALB/c mice
Brunner, R., Wallmann, J., Szalai, K., et al. (2007) · Clinical & Experimental Allergy · doi:10.1111/j.1365-2222.2007.02813.x · Cited by 35 · see citing works
Fall in Rectal Temperature as an Indication of Anaphylactic Shock in the Mouse
Kind, L. S. (1955) · The Journal of Immunology · doi:10.4049/jimmunol.74.5.387 · Cited by 20 · see citing works
von Garnier, C., Astori, M., Kettner, A., et al. (2002) · Clinical & Experimental Allergy · doi:10.1046/j.1365-2222.2002.01304.x · Cited by 16 · see citing works
Effect of daily oral omeprazole on 24 hour intragastric acidity.
Prichard, P. J., Yeomans, N. D., Mihaly, G. W., et al. (1983) · BMJ · doi:10.1136/bmj.287.6402.1378-c · Cited by 15 · see citing works
Eosinophils Accumulate in the Gastric Mucosa of Food-Allergic Mice
Untersmayr, E., Ellinger, A., Beil, W. J., et al. (2004) · International Archives of Allergy and Immunology · doi:10.1159/000080035 · Cited by 13 · see citing works
Self-citationWakabayashi, A., Utsuyama, M., Hosoda, T., et al. (2006) · PubMed · Cited by 9 · see citing works
The influence of antigen digestion on orally induced immunity and tolerance.
Jain, S. L., & Michael, J. G. (1995) · PubMed · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Use of lectin-functionalized particles for oral immunotherapy
Diesner, S. C., Wang, X.-Y., Jensen-Jarolim, E., Untersmayr, E., & Gabor, F. (2012) · Therapeutic Delivery · doi:10.4155/tde.11.146 · Cited by 40 · see citing works
Self-citationDiesner, S. C., Bergmayr, C., Wang, X.-Y., Heiden, D., Exenberger, S., Roth-Walter, F., Starkl, P., Ret, D., Pali-Schöll, I., Gabor, F., et al. (2018) · Clinical Immunology · doi:10.1016/j.clim.2018.03.017 · Cited by 9 · see citing works
Self-citation
- Authors
- Diesner, S. C., Knittelfelder, R., Krishnamurthy, D., Pali-Schöll, I., Gajdzik, L., Jensen-Jarolim, E., & Untersmayr, E.
- Affiliations
- Medical University of Vienna (AT)
- Corresponding author
- Untersmayr, E.
- Venue
- Immunology Letters
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 121
- Issue
- 1
- Pages
- 45-51
- ISSN
- 0165-2478 (ISSN-L)
- PMC
- PMC2999746
- OpenAlex
- W2063277409
- Cited by
- 81
- References
- 39
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 2 · 2020: 2 · 2019: 6 · 2018: 7 · 2017: 4 · 2016: 9 · 2015: 4 · 2014: 6 · 2013: 9 · 2012: 9
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Immunology Letters (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Oesterreichische Nationalbank
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
- Health Sciences › Medicine › Pharmacology › Drug-Induced Adverse Reactions
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Keywords
- food allergy, ovalbumin, proton pump inhibitors, gastric pH, oral immunization, intraperitoneal immunization, IgE, IgG1, IgG2a, anaphylaxis
- MeSH terms
- Administration, Oral, Animals, Disease Models, Animal, Female, Food Hypersensitivity, Gastric Acidity Determination, Hydrogen-Ion Concentration, Immunoglobulin E, Immunoglobulin G, Interferon-gamma, Mice, Inbred BALB C, Ovalbumin, Skin Tests, Interleukin-5, Mice, Proton Pump Inhibitors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
