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Nlrp6- and ASC-Dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition
Open accessAbstract
The gut microbiota regulate susceptibility to multiple human diseases. The Nlrp6-ASC inflammasome is widely regarded as a hallmark host innate immune axis that shapes the gut microbiota composition. This notion stems from studies reporting dysbiosis in mice lacking these inflammasome components when compared with non-littermate wild-type animals. Here, we describe microbial analyses in inflammasome-deficient mice while minimizing non-genetic confounders using littermate-controlled Nlrp6-deficient mice and ex-germ-free littermate-controlled ASC-deficient mice that were all allowed to shape their gut microbiota naturally after birth. Careful microbial phylogenetic analyses of these cohorts failed to reveal regulation of the gut microbiota composition by the Nlrp6- and ASC-dependent inflammasomes. Our results obtained in two geographically separated animal facilities dismiss a generalizable impact of Nlrp6- and ASC-dependent inflammasomes on the composition of the commensal gut microbiota and highlight the necessity for littermate-controlled experimental design in assessing the influence of host immunity on gut microbial ecology.
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“In fact, in different facilities, mice lacking ASC, NLRP6 (199), or NLRP3 (200) fail to show any detectable differences in the composition of their fecal flora compared to wild-type controlled littermates (199), either co-housed or individually housed, contrary to what has been described in other facilities (194).”
From The gut-brain axis: how microbiota and host inflammasome influence brain physiology and pathology (2020) · cites this paper as [199]
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Metadata
- Authors
- Mamantopoulos, M., Ronchi, F., Van Hauwermeiren, F., Vieira-Silva, S., Yilmaz, B., Martens, L., Saeys, Y., Drexler, S. K., Yazdi, A. S., Raes, J., Lamkanfi, M., McCoy, K. D., & Wullaert, A.
- Affiliations
- Ghent University (BE)
- University of Bern (CH)
- University of Basel (CH)
- University of Tübingen (DE)
- VIB-KU Leuven Center for Microbiology (BE)
- Corresponding author
- McCoy, Kathy D.
- Wullaert, Andy
- ORCID
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2017-08
- Volume
- 47
- Issue
- 2
- Pages
- 339-348.e4
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W2742175177
- Cited by
- 181
- References
- 39
- Citations by year
- 2026: 4 · 2025: 14 · 2024: 18 · 2023: 14 · 2022: 17 · 2021: 15 · 2020: 28 · 2019: 33 · 2018: 28 · 2017: 9
- FWCI
- 10.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- BORIS (University Library Bern) (submittedVersion)
- Digital Access to Libraries (submittedVersion, other-oa)
- Lirias (submittedVersion)
- Funders
- SNSF (SNSF310030_134902, PMPDP3_145453)
- European Research Council (FP/2007-2013, 281785, 281600, 683144)
- Marie Heim-Vögtlin fellowship
- ECCO (Odysseus grant G.0C49.13N)
- Fund for Scientific Research-Flanders
- Ghent University Concerted Research Actions (BOF14/GOA/013)
- Baillet Latour Medical Research Grant
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- NLRP6, ASC, inflammasome, gut microbiota composition, commensal microbiota, host innate immunity, dysbiosis, germ-free mice, host-microbe interactions
- MeSH terms
- Genetic Background, Gastrointestinal Microbiome, Animals, Bacteria, Cells, Cultured, Colitis, Female, Immunity, Innate, Mice, Inbred C57BL, Receptors, Cell Surface, RNA, Ribosomal, 16S, Sodium Dodecyl Sulfate, Mice, Knockout, Apoptosis Regulatory Proteins, Mice, CARD Signaling Adaptor Proteins, Inflammasomes, Microbiota, Dysbiosis
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