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Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity
Open accessAbstract
SUMMARY Stromal cells (SCs) establish the compartmentalization of lymphoid tissues critical to the immune response. However, the full diversity of lymph node (LN) SCs remains undefined. Using droplet‐based single‐cell RNA sequencing, we identified nine peripheral LN non‐endothelial SC clusters. Included are the established subsets, Ccl19hi T‐zone reticular cells (TRCs), marginal reticular cells, follicular dendritic cells (FDCs), and perivascular cells. We also identified Ccl19lo TRCs, likely including cholesterol‐25‐hydroxylase+ cells located at the T‐zone perimeter, Cxcl9+ TRCs in the T‐zone and interfollicular region, CD34+ SCs in the capsule and medullary vessel adventitia, indolethylamine N‐methyltransferase+ SCs in the medullary cords, and Nr4a1+ SCs in several niches. These data help define how transcriptionally distinct LN SCs support niche‐restricted immune functions and provide evidence that many SCs are in an activated state. Graphical Abstract Figure. No Caption available. HighlightsSingle‐cell RNA sequencing of lymph node stromal cells reveals nine clustersKnown subsets TRCs, MRCs, FDCs, and perivascular cells identifiedFive additional stromal cell clusters identified and anatomical locations determinedTwo clusters, Cxcl9+ TRCs and Nr4a1+ SCs, are defined by activation signatures &NA; Lymph node stromal cells support diverse processes, but bulk assessments obscure their niche‐specific functions. Rodda et al. identify transcriptional profiles for nine lymph node stromal cell clusters using single‐cell RNA sequencing, validate subset markers in situ, and suggest niche‐restricted functions.
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Metadata
- Authors
- Rodda, L. B., Lu, E., Bennett, M. L., Sokol, C. L., Wang, X., Luther, S. A., Barres, B. A., Luster, A. D., Ye, C. J., & Cyster, J. G.
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2018-05
- Volume
- 48
- Issue
- 5
- Pages
- 1014-1028.e6
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W2801762127
- Cited by
- 438
- References
- 81
- FWCI
- 15.46
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rodda, L. B., Lu, E., Bennett, M. L., Sokol, C. L., Wang, X., Luther, S. A., Barres, B. A., Luster, A. D., Ye, C. J., & Cyster, J. G. (2018). Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity. Immunity. https://doi.org/10.1016/j.immuni.2018.04.006
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