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Cited by 30Gut microbiota and healthNatural Compounds in Disease TreatmentT-cell and B-cell Immunology

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Regulation of gut microbiota substantially contributes to the induction of intestinal Treg cells and consequent anti-arthritis effect of madecassoside

Qiao, Simiao, Lian, Xingpan, Yue, Mengfan, Zhang, Qin, Wei, Zhifeng, Chen, Li, Xia, Yufeng, Dai, Yue·2020·International Immunopharmacology
DOI 10.1016/j.intimp.2020.107047
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Abstract

Previously, we reported that oral administration of madecassoside, the main active triterpene in Centella asiatica L., exerted anti-arthritis effect by inducing the generation of regulatory T (Treg) cells in small intestine. This study investigates the action site and mechanism of madecassoside to induce Treg cells. In collagen-induced arthritis (CIA) of rats, oral administration of madecassoside significantly alleviated arthritis symptoms, but its main metabolite madecassic acid did not, suggesting that madecassoside functions in the parent form. Madecassoside was shown to increase the number of Treg cells and promote the expression of Foxp3 and IL-10 in rat ileum rather than duodenum and jejunum, as detected using the immunohistochemistry assay and quantitative PCR assay, respectively. Unexpectedly, madecassoside was absent of significant effect on in vitro Treg cell differentiation and the expression of Foxp3 and IL-10. A combined use of broad-spectrum antibiotics resulted in significant reduction of the anti-arthritis effect of madecassoside in CIA rats. The 16S rRNA gene sequence showed that madecassoside could reverse the changes of gut microbiota under arthritis condition, and enrich several bacteria such as Lachnospiraceae, Butyricicoccus, Faecalibacterium, Butyricicoccus pullicaecorum and so on. GC-MS assay showed that madecassoside elevated the levels of acetic acid and butyric acid, but not other short chain fatty acids (SCFAs) in the cecum contents of CIA rats. Bu…

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  • Multiple studies verify role of orally administered madecassoside in protecting against arthritis by inhibiting pro-inflammatory cytokines and upregulating anti-inflammatory ones.

    “Furthermore, multiple studies verify the role of orally administered madecassoside in protecting against arthritis by similar action of inhibiting pro‐inflammatory cytokines and upregulating anti‐inflammatory ones.”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [138]

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Metadata

Authors
Qiao, S., Lian, X., Yue, M., Zhang, Q., Wei, Z., Chen, L., Xia, Y., & Dai, Y.
Affiliations
China Pharmaceutical University (CN)
Corresponding author
  • Xia, Yufeng
  • Dai, Yue
ORCID
Venue
International Immunopharmacology
Publisher
Elsevier BV
Published
2020-12
Volume
89
Issue
Pt A
Pages
107047
ISSN
1567-5769 (ISSN-L)
DOI
10.1016/j.intimp.2020.107047
OpenAlex
W3092079686
Cited by
30
References
1
Citations by year
2026: 3 · 2025: 10 · 2024: 2 · 2023: 8 · 2022: 5 · 2021: 2
FWCI
1.22
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Immunopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Double First Class University Plan
  • National Natural Science Foundation of China
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
madecassoside, Centella asiatica, gut microbiota, regulatory T cells, collagen-induced arthritis, butyrate, short-chain fatty acids, Foxp3, IL-10, butyrate-producing bacteria, Faecalibacterium, Lachnospiraceae, ileum
MeSH terms
Gastrointestinal Microbiome, Animals, Anti-Inflammatory Agents, Arthritis, Experimental, Female, Triterpenes, Molecular Structure, Rats, Wistar, T-Lymphocytes, Regulatory, Rats

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