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Cited by 34MicroRNA in disease regulationLipid metabolism and disordersLipid metabolism and biosynthesis

MicroRNA-124 Regulates Fatty Acid and Triglyceride Homeostasis

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Shaw, Tyler A., Singaravelu, Ragunath, Powdrill, Megan H., Nhan, Jordan, Ahmed, Nadine, Özcelik, Dennis, Pezacki, John Paul·2018·iScience
DOI 10.1016/j.isci.2018.11.028PMC PMC6282456
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Abstract

MicroRNAs (miRNAs) are part of a complex regulatory network that modulates cellular lipid metabolism. Here, we identify miR-124 as a regulator of triglyceride (TG) metabolism. This study advances our knowledge of the role of miR-124 in human hepatoma cells. Transcriptional profiling of Huh7.5 cells overexpressing miR-124 reveals enrichment for host factors involved in fatty acid oxidation among repressed miRNA targets. In addition, miR-124 down-regulates arylacetamide deacetylase (AADAC) and adipose triglyceride lipase, lipases proposed to mediate breakdown of hepatic TG stores for lipoprotein assembly and mitochondrial β-oxidation. Consistent with the inhibition of TG and fatty acid catabolism, miR-124 expression promotes cellular TG accumulation. Interestingly, miR-124 inhibits the production of hepatitis C virus, a virus that hijacks lipid pathways during its life cycle. Antiviral activity of miR-124 is consistent with repression of AADAC, a pro-viral host factor. Overall, our data highlight miR-124 as a novel regulator of TG metabolism in human hepatoma cells.

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  • Such mechanisms may not be microglia-specific because miR-124 has been shown to increase fatty acid and triglyceride content in liver cells.

    “However, such mechanisms may not be microglia-specific due to the fact that miR-124 has been shown to increase fatty acid and triglyceride content in liver cells [66, 67].”

    From HIV-TAT dysregulates microglial lipid metabolism through SREBP2/miR-124 axis: Implication of lipid droplet accumulation microglia in NeuroHIV (2025) · cites this paper as [66]

    The Results section, “miR-124 Promotes Cellular TG Accumulation” (page not provided), reports significantly increased triglyceride content in miR-124-treated Huh7.5 liver cells. It discusses free fatty acid accumulation as a rationale but does not report measuring an increase in fatty acid content, so that part of the claim is not established. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

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Authors
Shaw, T. A., Singaravelu, R., Powdrill, M. H., Nhan, J., Ahmed, N., Özcelik, D., & Pezacki, J. P.
Affiliations
University of Ottawa (CA)
Corresponding author
Pezacki, John Paul
ORCID
Venue
iScience
Publisher
Elsevier BV
Published
2018-12
Volume
10
Pages
149-157
ISSN
2589-0042 (ISSN-L)
DOI
10.1016/j.isci.2018.11.028
PMC
PMC6282456
OpenAlex
W2901951586
Cited by
34
References
4
Citations by year
2026: 2 · 2025: 6 · 2024: 4 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 8 · 2019: 2
FWCI
0.98
Type
journal article
Language
en
Open access
Licence
Locations
  • iScience (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Canadian Institutes of Health Research
  • Natural Sciences and Engineering Research Council of Canada
  • Canadian Network
  • Life Science Research Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
miR-124, triglyceride metabolism, fatty acid homeostasis, arylacetamide deacetylase, adipose triglyceride lipase, fatty acid oxidation, hepatitis C virus, antiviral activity, host factors, lipid metabolism, triglyceride accumulation

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