The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
The Mechanosensitive Ion Channel Piezo1 Significantly Mediates In Vitro Ultrasonic Stimulation of Neurons
Open accessAbstract
Ultrasound brain stimulation is a promising modality for probing brain function and treating brain disease non-invasively and with high spatiotemporal resolution.However, the mechanism underlying its effects remains unclear.Here, we examine the role that the mouse piezo-type mechanosensitive ion channel component 1 (Piezo1) plays in mediating the in vitro effects of ultrasound in mouse primary cortical neurons and a neuronal cell line.We show that ultrasound alone could activate heterologous and endogenous Piezo1, initiating calcium influx and increased nuclear c-Fos expression in primary neurons but not when pre-treated with a Piezo1 inhibitor.We also found that ultrasound significantly increased the expression of the important proteins phospho-CaMKII, phospho-CREB, and c-Fos in a neuronal cell line, but Piezo1 knockdown significantly reduced this effect.Our findings demonstrate that the activity of mechanosensitive ion channels such as Piezo1 stimulated by ultrasound is an important contributor to its ability to stimulate cells in vitro.Among the possible ultrasound-responsive mechanosensitive ion channels, members of the Piezo family are eminent candidates (Coste et al., 2010).The Piezos are very large, evolutionarily conserved transmembrane proteins, and Piezo1 is among the channels most sensitive to physical force (Cox et al., 2017).It can activate and deactivate in the range of milliseconds (Coste et al., 2010) to forces estimated to be as low as
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Piezo1 and Piezo2 Are Essential Components of Distinct Mechanically Activated Cation Channels
Coste, B., Mathur, J., Schmidt, M., Earley, T. J., Ranade, S., Petrus, M. J., Dubin, A. E., & Patapoutian, A. (2010) · Science · doi:10.1126/science.1193270 · Cited by 3,316
The regulation and function of c-fos and other immediate early genes in the nervous system
Sheng, M., & Greenberg, M. E. (1990) · Neuron · doi:10.1016/0896-6273(90)90106-p · Cited by 2,429
Piezo1 integration of vascular architecture with physiological force
Li, J., Hou, B., Tumova, S., Muraki, K., Bruns, A., Ludlow, M. J., Sedo, A., Hyman, A. J., McKeown, L., Young, R. S., et al. (2014) · Nature · doi:10.1038/nature13701 · Cited by 1,223
Transcranial focused ultrasound modulates the activity of primary somatosensory cortex in humans
Legon, W., Sato, T. F., Opitz, A., Mueller, J., Barbour, A., Williams, A., & Tyler, W. J. (2014) · Nature Neuroscience · doi:10.1038/nn.3620 · Cited by 981
Transcranial Pulsed Ultrasound Stimulates Intact Brain Circuits
Tufail, Y., Matyushov, A., Baldwin, N., Tauchmann, M. L., Georges, J., Yoshihiro, A., Tillery, S. I. H., & Tyler, W. J. (2010) · Neuron · doi:10.1016/j.neuron.2010.05.008 · Cited by 977
Piezo1, a mechanically activated ion channel, is required for vascular development in mice
Ranade, S. S., Qiu, Z., Woo, S.-H., Hur, S. S., Murthy, S. E., Cahalan, S. M., Xu, J., Mathur, J., Bandell, M., Coste, B., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1409233111 · Cited by 901
Self-citationChemical activation of the mechanotransduction channel Piezo1
Syeda, R., Xu, J., Dubin, A. E., Coste, B., Mathur, J., Huynh, T., Matzen, J., Lao, J., Tully, D. C., Engels, I. H., et al. (2015) · eLife · doi:10.7554/elife.07369 · Cited by 737
Remote Excitation of Neuronal Circuits Using Low-Intensity, Low-Frequency Ultrasound
Tyler, W. J., Tufail, Y., Finsterwald, M., Tauchmann, M. L., Olson, E. J., & Majestic, C. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003511 · Cited by 707
Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells
Pathak, M. M., Nourse, J. L., Tran, T., Hwe, J., Arulmoli, J., Le, D. T. T., Bernardis, E., Flanagan, L. A., & Tombola, F. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1409802111 · Cited by 657
Mechanosensing is critical for axon growth in the developing brain
Koser, D. E., Thompson, A. J., Foster, S. K., Dwivedy, A., Pillai, E. K., Sheridan, G. K., Svoboda, H., Viana, M., Costa, L. D. F., Guck, J., et al. (2016) · Nature Neuroscience · doi:10.1038/nn.4394 · Cited by 649
Cited by
Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets
Di, X., Gao, X., Peng, L., Ai, J., Jin, X., Qi, S., Li, H., Wang, K., & Luo, D. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01501-9 · Cited by 622
Ultrasound-responsive systems as components for smart materials
Athanassiadis, A. G., Ma, Z., Moreno-Gomez, N., Melde, K., Choi, E., Goyal, R., & Fischer, P. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00622 · Cited by 293
A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel
Geng, J., Liu, W., Zhou, H., Zhang, T., Wang, L., Zhang, M., Li, Y., Shen, B., Li, X., & Xiao, B. (2020) · Neuron · doi:10.1016/j.neuron.2020.02.010 · Cited by 113
Song, H., Kim, M., Kim, E., Lee, J., Jeong, I., Lim, K., Ryu, S. Y., Oh, M., Kim, Y., & Park, J. (2024) · BMEMat · doi:10.1002/bmm2.12048 · Cited by 61
The Emergence of Functional Ultrasound for Noninvasive Brain–Computer Interface
Zheng, H., Niu, L., Qiu, W., Liang, D., Long, X., Li, G., Liu, Z., & Meng, L. (2023) · Research · doi:10.34133/research.0200 · Cited by 27
Self-citation
Metadata
- Authors
- Qiu, Z., Guo, J., Kala, S., Zhu, J., Xian, Q., Qiu, W., Li, G., Zhu, T., Meng, L., Zhang, R., Chan, H. C., Zheng, H., & Sun, L.
- Venue
- iScience
- Publisher
- Elsevier BV
- Published
- 2019-11
- Volume
- 21
- Pages
- 448-457
- ISSN
- 2589-0042 (ISSN-L)
- OpenAlex
- W2948777239
- Cited by
- 266
- References
- 48
- FWCI
- 7.13
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Mechanosensitive Ion Channel Piezo1 Significantly Mediates In Vitro Ultrasonic Stimulation of Neurons. https://app.cochat.ai/papers/10.1016/j.isci.2019.10.037
- The paper
- Qiu, Z., Guo, J., Kala, S., Zhu, J., Xian, Q., Qiu, W., Li, G., Zhu, T., Meng, L., Zhang, R., Chan, H. C., Zheng, H., & Sun, L. (2019). The Mechanosensitive Ion Channel Piezo1 Significantly Mediates In Vitro Ultrasonic Stimulation of Neurons. iScience. https://doi.org/10.1016/j.isci.2019.10.037
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
