CoChat
Sign Up
Cited by 54Dermatology and Skin DiseasesAsthma and respiratory diseasesContact Dermatitis and Allergies

The prostaglandin D2 receptor CRTH2 is important for allergic skin inflammation after epicutaneous antigen challenge

Open access
He, Rui, Oyoshi, Michiko K., Wang, James Y.T., Hodge, Martin R., Jin, Haoli, Geha, Raif S.·2010·Journal of Allergy and Clinical Immunology
DOI 10.1016/j.jaci.2010.07.006PMC PMC2950231
Open this paper in CoChat — free account

Abstract

Background Cutaneous prostaglandin (PG) D₂ levels increase after scratching. Chemoattractant receptor-homologous molecule expressed on receptor on T(H)2 cells (CRTH2) mediates chemotaxis to PGD₂ and is expressed on T(H)2 cells and eosinophils, which infiltrate skin lesions in patients with atopic dermatitis.Objective We sought to examine the role of CRTH2 in a murine model of atopic dermatitis.Methods CRTH2(-/-) mice and wild-type control animals were epicutaneously sensitized by means of repeated application of ovalbumin (OVA) to tape-stripped skin for 7 weeks and then challenged by means of OVA application to tape-stripped previously unsensitized skin for 1 week. Skin histology was assessed by means of hematoxylin and eosin staining and immunohistochemistry. Cytokine mRNA expression was examined by means of quantitative RT-PCR. Levels of PGD₂, antibody, and cytokines were measured by means of ELISA.Results PGD₂ levels significantly increased in skin 24 hours after tape stripping, although not in skin subjected to repeated sensitization with OVA. Allergic skin inflammation developed normally at sites of chronic epicutaneous sensitization with OVA in CRTH2(-/-) mice but was severely impaired in previously unsensitized skin challenged with OVA, as evidenced by significantly decreased skin infiltration with eosinophils and CD4(+) cells and impaired T(H)2 cytokine mRNA expression. Impaired skin inflammation at sites of acute OVA challenge in CRTH2(-/-) mice was not due to an impaired systemic response to epicutaneous sensitization because OVA-specific IgG1 and IgE antibody levels and OVA-driven splenocyte secretion of cytokines in these mice were comparable with those seen in wild-type control animals.Conclusions CRTH2 promotes allergic skin inflammation in response to cutaneous exposure to antigen in previously sensitized mice.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.jaci.2010.07.006

Cites

40 of 40 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In vitro and animal-model evidence indicates that CRTH2 has an important role in TH2-mediated AD inflammation by directing migration into the skin.

    “as shown in vitro and in animal models154,155, has an important role in TH2-mediated inflammation of AD by directing their migration into the skin.”

    From Atopic dermatitis: an expanding therapeutic pipeline for a complex disease (2022) · cites this paper as [155]

    The Introduction reports CRTH2-mediated chemotaxis in vitro, and the Results section on acute OVA challenge reports reduced skin infiltration by CD4+ cells and eosinophils in CRTH2-deficient mice (page numbers unavailable). But the Results on repeated sensitization find no important role for CRTH2 in chronic skin inflammation. The evidence therefore supports migration-related inflammation in an acute mouse model, not an unqualified role in AD inflammation. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
He, R., Oyoshi, M. K., Wang, J. Y. T., Hodge, M. R., Jin, H., & Geha, R. S.
Affiliations
  • Boston Children's Hospital (US)
  • Biogen (United States) (US)
Corresponding author
Geha, Raif S.
ORCID
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
2010-10
Volume
126
Issue
4
Pages
784-790
ISSN
0091-6749 (ISSN-L)
DOI
10.1016/j.jaci.2010.07.006
PMC
PMC2950231
OpenAlex
W2029473902
Cited by
54
References
40
Citations by year
2025: 3 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 8 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 7
FWCI
6.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (n01ai40030-7-0-0)
  • National Institute of Allergy and Infectious Diseases (N01 AI040030, AI 40030, N01 AI400030)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR047417, AR-047417)
Topics
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
Keywords
CRTH2, prostaglandin D2, allergic skin inflammation, atopic dermatitis, epicutaneous sensitization, ovalbumin, eosinophils, Th2 cells, IgE, cytokine expression
MeSH terms
Administration, Cutaneous, Animals, Chemotaxis, Leukocyte, Dermatitis, Atopic, Female, Humans, Mice, Inbred BALB C, Ovalbumin, Receptors, Immunologic, Receptors, Prostaglandin, Skin, Prostaglandin D2, Mice, Knockout, Th2 Cells, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review