The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Highly efficient gene targeting in the Aspergillus niger kusA mutant
Abstract
Gene targeting frequencies in Aspergillus niger are often very low and hamper efficient functional genomics in this biotechnologically important fungus. Deletion of the A. niger kusA gene encoding the ortholog of the Ku70 protein in other eukaryotes, dramatically improved homologous integration efficiency and reached more than 80% compared to 7% in the wild-type background, when 500bp homologous flanks were used. Furthermore, the use of the DeltakusA strain resulted in a high frequency of heterokaryon formation (70%) in primary transformants in the case disrupting an essential gene. Deletion of kusA had no obvious effect on the growth of the fungus, but renders the DeltakusA strain 10 times more sensitive to X-ray irradiation and two to three times more sensitive to UV exposure. The highly efficient gene targeting in combination with the A. niger genome sequence allows a systematic approach to generate gene knockouts and will help in improving the capacities of A. niger as producer of commercially interesting proteins and metabolites.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Recombination Proteins in Yeast
Krogh, B. O., & Symington, L. S. (2004) · Annual Review of Genetics · doi:10.1146/annurev.genet.38.072902.091500 · Cited by 805
A Versatile and Efficient Gene-Targeting System for Aspergillus nidulans
Nayak, T., Szewczyk, E., Oakley, C. E., Osmani, A., Ukil, L., Murray, S. L., Hynes, M. J., Osmani, S. A., & Oakley, B. R. (2006) · Genetics · doi:10.1534/genetics.105.052563 · Cited by 610
Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining
Ninomiya, Y., Suzuki, K., Ishii, C., & Inoue, H. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402780101 · Cited by 599
Bleomycin: New Perspectives on the Mechanism of Action
Hecht, S. M. (2000) · Journal of Natural Products · doi:10.1021/np990549f · Cited by 559
da Silva Ferreira, M. E., Kress, M. R. V. Z., Savoldi, M., Goldman, M. H. S., Härtl, A., Heinekamp, T., Brakhage, A. A., & Goldman, G. H. (2006) · Eukaryotic Cell · doi:10.1128/ec.5.1.207-211.2006 · Cited by 403
Development of a homologous transformation system for Aspergillus niger based on the pyrG gene
van Hartingsveldt, W., Mattern, I. E., van Zeijl, C. M. J., Pouwels, P. H., & van den Hondel, C. A. M. J. J. (1987) · Molecular and General Genetics MGG · doi:10.1007/bf00326538 · Cited by 336
Self-citationDNA double-strand breaks and alkali-labile bonds produced by bleomycin
Povirk, L. F., Wübker, W., Köhnlein, W., & Hutchinson, F. (1977) · Nucleic Acids Research · doi:10.1093/nar/4.10.3573 · Cited by 291
Krappmann, S., Sasse, C., & Braus, G. H. (2006) · Eukaryotic Cell · doi:10.1128/ec.5.1.212-215.2006 · Cited by 287
Osmani, S. A., Engle, D. B., Doonan, J. H., & Morris, N. R. (1988) · Cell · doi:10.1016/0092-8674(88)90513-2 · Cited by 231
Takahashi, T., Masuda, T., & Koyama, Y. (2006) · Molecular Genetics and Genomics · doi:10.1007/s00438-006-0104-1 · Cited by 181
Cited by
Genetic engineering of filamentous fungi — Progress, obstacles and future trends
Meyer, V. (2008) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.12.001 · Cited by 358
Self-citationNäätsaari, L., Mistlberger, B., Ruth, C., Hajek, T., Hartner, F. S., & Glieder, A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0039720 · Cited by 281
Efficient Gene Replacements in Toxoplasma gondii Strains Deficient for Nonhomologous End Joining
Fox, B. A., Ristuccia, J. G., Gigley, J. P., & Bzik, D. J. (2009) · Eukaryotic Cell · doi:10.1128/ec.00357-08 · Cited by 263
Garrigues, S., Peng, M., Kun, R. S., & de Vries, R. P. (2023) · mBio · doi:10.1128/mbio.00668-23 · Cited by 22
Metadata
- Authors
- Meyer, V., Arentshorst, M., El-Ghezal, A., Drews, A.-C., Kooistra, R., van den Hondel, C. A. M. J. J., & Ram, A. F. J.
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 128
- Issue
- 4
- Pages
- 770-775
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W1974618342
- Cited by
- 307
- References
- 20
- FWCI
- 6.47
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Highly efficient gene targeting in the Aspergillus niger kusA mutant. https://app.cochat.ai/papers/10.1016/j.jbiotec.2006.12.021
- The paper
- Meyer, V., Arentshorst, M., El-Ghezal, A., Drews, A.-C., Kooistra, R., van den Hondel, C. A. M. J. J., & Ram, A. F. J. (2007). Highly efficient gene targeting in the Aspergillus niger kusA mutant. Journal of Biotechnology. https://doi.org/10.1016/j.jbiotec.2006.12.021
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
