Detection of the osteogenic differentiation of mesenchymal stem cells in 2D and 3D cultures by electrochemical impedance spectroscopy
Abstract
Human mesenchymal stem cells are promising candidates for cell-based therapies since they have the capacity to differentiate into a variety of cell types. However, the acceptance of hMSCs for clinical applications as well as in vitro tissue models will depend on strategies for standard characterisations. Impedance spectroscopy is a proven and powerful tool for non-invasive monitoring of cellular processes. The aim of this study was to prove the hypothesis, that the process of osteogenic differentiation can be monitored non-invasively and time-continuously by using impedance spectroscopy. This hypothesis was examined for 2D cell layers of hMSCs by continuous impedance spectroscopy employing a planar electrode-based chip and for 3D aggregates of hMSCs after 21 and 25 days of osteogenic treatment by using a capillary measurement system. The impedance spectra of osteogenic treated hMSCs reported a significant increase of the magnitude of impedance compared to controls cultivated in normal growth medium. The osteogenic status of the cells was determined by alkaline phosphatase expression and von Kossa staining. In respect to that finding it is concluded that impedance spectroscopy is an appropriate method for non-invasive characterisation of osteogenic differentiation of hMSCs, which is relevant for quality control of cell-based implants and cell-based test systems for drug development.
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Hildebrandt et al. used the capillary system to measure the impedance of mesenchymal stem cell spheroids at three different conditions.
“Hildebrandt et al . used the capillary system to measure the impedance of mesenchymal stem cell ( MSC) spheroids at three different conditions. 90”
From Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors (2014) · cites this paper as [90]
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- Authors
- Hildebrandt, C., Büth, H., Cho, S., Impidjati, & Thielecke, H.
- Affiliations
- Fraunhofer Institute for Biomedical Engineering (DE)
- Corresponding author
- Thielecke, Hagen
- ORCID
- Cho, Sungbo
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 148
- Issue
- 1
- Pages
- 83-90
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W1985983525
- Cited by
- 95
- References
- 46
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 7 · 2019: 4 · 2018: 10 · 2017: 5 · 2016: 5 · 2015: 10 · 2014: 5 · 2013: 6 · 2012: 9
- FWCI
- 3.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft) (submittedVersion)
- Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- human mesenchymal stem cells, osteogenic differentiation, electrochemical impedance spectroscopy, non-invasive cell monitoring, 3D cell aggregates, alkaline phosphatase, von Kossa staining
- MeSH terms
- Aged, Cell Aggregation, Cell Differentiation, Cells, Cultured, Humans, Osteogenesis, Spectrum Analysis, Electric Impedance, Cell Culture Techniques, Mesenchymal Stem Cells
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