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Cited by 40Viral Infections and Immunology ResearchBiosensors and Analytical DetectionMolecular Biology Techniques and Applications

The DOI matches its Crossref record, with a small difference in title or year; 1 of the 1 references it cites were found in a registry or an index.

Performance of the GeneXpert® enterovirus assay for detection of enteroviral RNA in cerebrospinal fluid

Marlowe, Elizabeth M., Novak, Susan M., Dunn, James J., Smith, Amy, Cumpio, Joven, Makalintal, Edna, Barnes, Darryl, Burchette, Raoul J.·2008·Journal of Clinical Virology
DOI 10.1016/j.jcv.2008.04.006
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Abstract

Background The GeneXpert Dx System allows for automated extraction, processing, amplification and real-time detection of target nucleic acids. Objectives To evaluate the performance of the Cepheid Xpert enterovirus (EV) assay for detection of EV RNA compared to a nucleic acid sequence based amplification (NASBA) assay and a user-developed TaqMan RT-PCR assay. Study design Assays were evaluated using a 12-member proficiency panel and up to 138 CSF specimens. Samples in which EV RNA was detected by two or more assays were considered true positives. Results The GeneXpert, NASBA, and TaqMan assays correctly identified 10, 8, and 7 of 12 proficiency panel members, respectively. For detection of EV RNA in CSF, the sensitivities of the GeneXpert, NASBA, and TaqMan were 100%, 87.5%, and 96%, respectively. There were no false positives. Two samples tested by GeneXpert and NASBA yielded indeterminate or invalid results and could not be resolved. Conclusions The Xpert EV assay is a sensitive and specific method for detection of EV RNA in CSF specimens. The ease of use, random access capability, and minimal hands-on time with the automated GeneXpert system affords laboratories with little molecular diagnostics expertise an opportunity to complete a clinically useful testing within 2.5 h.

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Research works printed but not linked: 16

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  4. Kost, 2007, Multicenter beta trial of the GeneXpert enterovirus assay, J Clin Microbiol, 45, 1081, 10.1128/JCM.01718-06 · DOI
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Metadata

Authors
Marlowe, E. M., Novak, S. M., Dunn, J. J., Smith, A., Cumpio, J., Makalintal, E., Barnes, D., & Burchette, R. J.
Affiliations
  • Cook Children's Medical Center (US)
  • Kaiser Permanente (US)
Corresponding author
Marlowe, Elizabeth M.
ORCID
Venue
Journal of Clinical Virology
Publisher
Elsevier BV
Published
2008-09
Volume
43
Issue
1
Pages
110-113
ISSN
1386-6532 (ISSN-L)
DOI
10.1016/j.jcv.2008.04.006
OpenAlex
W2043776256
Cited by
40
References
1
Citations by year
2025: 1 · 2024: 1 · 2022: 6 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 6 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 1
FWCI
2.91
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Clinical Virology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Keywords
cerebrospinal fluid, TaqMan real-time PCR, assay sensitivity and specificity, proficiency panels, molecular diagnosis
MeSH terms
Enterovirus Infections, Enterovirus, Humans, RNA, Viral, Sensitivity and Specificity, Reverse Transcriptase Polymerase Chain Reaction, Self-Sustained Sequence Replication, Molecular Diagnostic Techniques

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