Influence of sensory neuropeptides on human cutaneous wound healing process
Abstract
Background Close interactions exist between primary sensory neurons of the peripheral nervous system (PNS) and skin cells. The PNS may be implicated in the modulation of different skin functions as wound healing.Objective Study the influence of sensory neurons in human cutaneous wound healing.Methods We incubated injured human skin explants either with rat primary sensory neurons from dorsal root ganglia (DRG) or different neuropeptides (vasoactive intestinal peptide or VIP, calcitonin gene-related peptide or CGRP, substance P or SP) at various concentrations. Then we evaluated their effects on the proliferative and extracellular matrix (ECM) remodeling phases, dermal fibroblasts adhesion and differentiation into myofibroblasts.Results Thus, DRG and all studied neuromediators increased fibroblasts and keratinocytes proliferation and act on the expression ratio between collagen type I and type III in favor of collagen I, particularly between the 3rd and 7th day of culture. Furthermore, the enzymatic activities of matrix metalloprotesases (MMP-2 and MMP-9) were increased in the first days of wound healing process. Finally, the adhesion of human dermal fibroblasts and their differentiation into myofibroblasts were promoted after incubation with neuromediators. Interestingly, the most potent concentrations for each tested molecules, were the lowest concentrations, corresponding to physiological concentrations.Conclusion Sensory neurons and their derived-neuropeptides are able to promote skin wound healing.
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Co-culture with rat primary sensory neurons from DRG as well as SP, CGRP and VIP facilitate fibroblast and keratinocyte proliferation and induce collagen I production.
“Co-culture with rat primary sensory neurons from DRG and as well as SP, CGRP and vasoactive intestinal peptide or VIP facilitate fibroblasts and keratinocytes proliferation and induce collagen I production81.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [81]
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- Authors
- Chéret, J., Lebonvallet, N., Buhé, V., Carre, J. L., Misery, L., & Le Gall-Ianotto, C.
- Affiliations
- Université de Bretagne Occidentale (FR)
- Corresponding author
- Misery, L.
- Venue
- Journal of Dermatological Science
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 74
- Issue
- 3
- Pages
- 193-203
- ISSN
- 0923-1811 (ISSN-L)
- OpenAlex
- W2084961143
- Cited by
- 103
- References
- 62
- Citations by year
- 2026: 10 · 2025: 7 · 2024: 9 · 2023: 6 · 2022: 11 · 2021: 12 · 2020: 9 · 2019: 8 · 2018: 5 · 2017: 8 · 2016: 7 · 2015: 8 · 2014: 3
- FWCI
- 8.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Dermatological Science (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- sensory neuropeptides, skin wound healing, primary sensory neurons, dorsal root ganglia, vasoactive intestinal peptide, calcitonin gene-related peptide, substance P, dermal fibroblasts, myofibroblast differentiation, keratinocyte proliferation, extracellular matrix remodeling, type I collagen, matrix metalloproteinases, MMP-2, MMP-9, skin explants
- MeSH terms
- Animals, Cell Adhesion, Cell Differentiation, Collagen, Humans, Neuropeptides, Sensory Receptor Cells, Skin, Wound Healing, Coculture Techniques, Matrix Metalloproteinases, Cell Proliferation, Rats, Myofibroblasts, Re-Epithelialization, In Vitro Techniques
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