The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Antioxidant capacity of flavonoids from Folium Artemisiae Argyi and the molecular mechanism in Caenorhabditis elegans
Abstract
Ethnopharmacological relevance Folium Artemisiae Argyi (FAA) is one kind of Chinese herbal medicine with a long history. It has widespread pharmacological activities such as antibacterial, anti-inflammatory, antioxidative, and hemostatic, among others. FAA is traditionally used for the treatment of eczema, respiratory diseases and gynecological diseases for hundreds of years. Flavonoids are reported as the main components of them. Recent studies focused on the antioxidant effect of its flavonoids in vitro, while few studies focused on the antioxidant effect in vivo, and the underlying mechanisms have not yet been elucidated.Aim of the study The aim of this study was to evaluate the antioxidant activity of Folium Artemisia Argyi flavonoids (FAAF) and explore its possible molecular mechanism in Caenorhabditis elegans. The research and development of its medicinal value will beneficial to the resource utilization of FAA.Materials and methods Firstly, FAAF was prepared, purified and then qualitatively and quantitatively analyzed using LC-DAD-MS. Then, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), hydroxyl radical and ferric reducing antioxidant power (FRAP) assays were applied to investigate the antioxidant effect of FAAF in vitro. Meanwhile, a stress resistance assay was carried out to evaluate the antioxidant effect of FAAF in vivo. Moreover, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and reactive oxygen species (ROS) accumulation were determined to ascertain whether FAAF can increase the oxidant defense system of nematodes and reduce the accumulation of ROS. Lipofuscin and protein carbonylation assays were employed to test whether FAAF can increase the antioxidant capacity of nematodes to resist the growth health indicators related to antioxidation. At last, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate the expression of genes related to antioxidation. The expression of green fluorescent protein (GFP) was further investigated using a fluorescence microscope in transgenic strains (SOD-3::GFP, GST-4::GFP, and HSP-16.2::GFP).Results FAAF exhibited a strong antioxidant capacity and enhanced stress resistance in C. elegans. FAAF reduced ROS accumulation and improved the antioxidant defense system under acute stress. Moreover, FAAF prevented the accumulation of lipofuscin and protein carbonylation in C. elegans. FAAF also upregulated the gene expression levels of hsp-16.2, gst-4, sod-3, skn-1, daf-16, ctl-2, hsf-1 and increased SOD-3::GFP and GST-4::GFP expression.Conclusion These results demonstrated that FAAF exerted antioxidant activity in C. elegans. It was perhaps regulated by the insulin/insulin-like growth factor-1(IGF-1) signaling pathway.
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“The significant presence of flavonoids in the ethanolic extracts, especially from the North and South Zones, aligns with their superior antioxidant properties in the TFC assay [19,35].”
From Comparative Analysis of Infusions and Ethanolic Extracts of Annona muricata Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity (2024) · cites this paper as [35]
Citations
Cites
Oxidants, oxidative stress and the biology of ageing
Finkel, T., & Holbrook, N. J. (2000) · Nature · doi:10.1038/35041687 · Cited by 9,413
ROS function in redox signaling and oxidative stress
Schieber, M., & Chandel, N. S. (2014) · Current Biology · doi:10.1016/j.cub.2014.03.034 · Cited by 6,931
Lin, K., Hsin, H., Libina, N., & Kenyon, C. J. (2001) · Nature Genetics · doi:10.1038/88850 · Cited by 1,027
DAF-16 Target Genes That Control C. elegans Life-Span and Metabolism
Lee, S. S., Kennedy, S., Tolonen, A. C., & Ruvkun, G. (2003) · Science · doi:10.1126/science.1083614 · Cited by 669
Stress resistance as a determinate of C. elegans lifespan
Lithgow, G. J., & Walker, G. A. (2002) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(01)00422-5 · Cited by 212
Worming pathways to and from DAF-16/FOXO
Mukhopadhyay, A., Oh, S. W., & Tissenbaum, H. A. (2006) · Experimental Gerontology · doi:10.1016/j.exger.2006.05.020 · Cited by 198
Ruan, D., Wang, W., Lin, C., Fouad, A. M., Chen, W., Xia, W., Wang, S., Luo, X., Zhang, W. H., Yan, S., et al. (2018) · animal · doi:10.1017/s1751731118000678 · Cited by 106
Sobeh, M., Mahmoud, M. F., Abdelfattah, M. A. O., Cheng, H., El-Shazly, A. M., & Wink, M. (2017) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2017.11.007 · Cited by 104
Oxidative stress-a key emerging impact factor in health, ageing, lifestyle and aesthetics
Kandola, K., Bowman, A., & Birch‐Machin, M. A. (2015) · International Journal of Cosmetic Science · doi:10.1111/ics.12287 · Cited by 103
Chen, L., Zhang, H., Chao, J., & Liu, J. (2017) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2017.04.017 · Cited by 98
Research works printed but not linked: 12
- Bao, 2018, Studies on the antioxidant activity of flavonoids from Osmanthus fragrans in vitro and in vitro, Food Res. Dev., 39, 14
- Gong, 2011, Experimental study on long-term toxicity of different components of Artemisia argyi leaves to normal rats, Chin. J. Pharm., 8, 401
- Hu, 2012, Advances in pharmacological effects and mechanism of Artemisia in the prevention and treatment of gastrointestinal diseases, Drugs Clinic, 27, 304
- Huang, 2017, Study on the contents of brown caryophyllin and isophoraxanthin in Artemisia argyi leaves from different habitats recorded in ancient Chinese materia Medica, China J. Chin. Mater. Med., 42, 3504
- Jin, 2018, Studies on the toxic effects of three commonly used preservatives on Caenorhabditis elegans, J. Food Safety Qual., 9, 905
- Luo, 2020, Based on UPLC-Q-TOF-MS analysis, the chemical constituents of Artemisia annua L. and Artemisia argyi were compared, China J. Chin. Mater. Med., 45, 4057
- Moliner, 2020, vol. 9
- Sun, 2010, Comparative study on acute toxicity of different components of Artemisia argyi in mice, Chin. J. Pharm., 7, 392
- Sun, 2020, Optimization of extraction technology of flavonoids from wild jujube leaves and its activity against oxidative damage of C. elegans, Sci. Technol. Food Indus., 41, 143
- Wang, 2019, Qualitative and quantitative analysis of phenolic acids and flavonoids in Artemisia argyi by UPLC-DAD-MS, China J. Chin. Mater. Med., 44, 983
- Wang, 2015, Research progress on the relationship between protein carbonyl and aging diseases, Chin. J. Gen. Surg., 13, 47
- Yuan, 2019, Studies on the antioxidant activity of flavonoid compounds from black olive leaves in vitro and in vivo, Food Res. Dev., 40, 46
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Cao, Y., Ren, S., Li, X., Xiao, X., & Xiao, Y. (2026) · Frontiers in Pharmacology · doi:10.3389/fphar.2026.1816985 · Cited by 1
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Jo, Y., & Cho, J. H. (2024) · Journal of the Korean Society of Food Science and Nutrition · doi:10.3746/jkfn.2024.53.12.1252
Zhang, Y., Zhao, D., Liu, X., Sun, S., Zheng, L., Liu, G., Chen, Y., Xu, H., Tian, Y., & Feng, S. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5380926
Ma, R., Zhang, R., Feng, W., Liu, X., Lian, C., Liu, J., Yang, J., Gui, X., & chen, S. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5393307
Zhang, Q., Zhang, X., Jiang, Q., Li, X., & Jiang, M. (2025) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.10129
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Liu, P., Zhong, L., Xiao, J., Hu, Y., Liu, T., Ren, Z., Wang, Y., & Zheng, K. (2023) · Figshare · doi:10.6084/m9.figshare.22606427
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Li, J., Shi, L., Ren, Z., & Weng, W. (2026) · Food Chemistry · doi:10.1016/j.foodchem.2026.148685
Ouyang, B., Wu, Q., Liu, F., Liang, J., Ma, W., Zhao, Y., Cao, H., & Xiao, J. (2026) · AgriFood Journal of Agricultural Products for Food · doi:10.1002/agf2.70014 · Cited by 1
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Shi, X., Yang, S., Dong, Y.-L., Li, X., Meng, L., Li, Y., Wang, D., Zhou, X.-W., & Wang, B. (2026) · Phytochemistry · doi:10.1016/j.phytochem.2026.114908
Ma, M., Lee, Y. J., Park, J., Son, D., KIM, H. K., Jo, Y.-H., Lee, S. B., Kim, G., Choi, E., Seo, M., et al. (2026) · Food Science & Nutrition · doi:10.1002/fsn3.71764
ZHANG, J., Yumin, S. H. A. N., Wang, Y., Hou, X., Liu, Y., Zhang, H., Wang, H., Simal‐Gándara, J., Xiao, J., & Cao, H. (2026) · Food Research International · doi:10.1016/j.foodres.2026.119842
Ferreira, B. R., Campos, M. F., Rodrigues, S. B. F., Lima, A. B., Mendonça, S. C., Vilanova, C. M., Coutinho, D. F., Allonso, D., do Amaral, F. M. M., & Leitão, S. G. (2026) · Plants · doi:10.3390/plants15131998
Zhou, J., Yang, C., Guo, Y., Zhao, L., Lu, S., Luo, L., Zhou, D., & Yin, Z. (2026) · Nutrition & Metabolism · doi:10.1186/s12986-026-01173-6
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Cui, X., Luo, J., Ding, X., Zeng, Q., Bai, S., Mao, X., Jia, G., Liu, Y., & Wang, J. (2026) · Poultry Science · doi:10.1016/j.psj.2026.107624
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Wang, D. (2026) · Springer eBooks · doi:10.1007/978-981-92-5166-7_7
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Wang, D. (2026) · Springer eBooks · doi:10.1007/978-981-92-5166-7_11
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Wang, D. (2026) · Springer eBooks · doi:10.1007/978-981-92-5166-7_4
Liu, Y., Wen, X., Li, R., & He, Y. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.110055
Xu, M., Ma, Z., Liu, J., Wang, L., Ocholi, S. S., Zhang, Z., Wang, F., & Han, L. (2026) · Journal of Chromatography B · doi:10.1016/j.jchromb.2026.125314
Wan, J., Wang, Y., Yang, X., & Guo, Q. (2026) · Chemistry & Biodiversity · doi:10.1002/cbdv.71686
Santos, P. A., de Andrade, E. M., Siqueira, I. R., & Pereira, P. (2026) · Molecular Neurobiology · doi:10.1007/s12035-026-06212-0
Huang, L., Yin, F., Fu, X., Huang, Y., Tang, Y., Liao, G., Wang, B., Yang, T., Huang, G., & Chen, X. (2026) · Biogerontology · doi:10.1007/s10522-026-10507-z
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Metadata
- Authors
- Hu, Q., Liu, Z., Guo, Y., Lu, S., Du, H., & Cao, Y.
- Affiliations
- Hubei University of Chinese Medicine (CN)
- Hubei University of Medicine (CN)
- Corresponding author
- Cao, Yan
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 2021-10
- Volume
- 279
- Pages
- 114398
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W3182293960
- Cited by
- 139
- References
- 28
- Citations by year
- 2026: 30 · 2025: 35 · 2024: 35 · 2023: 27 · 2022: 12
- FWCI
- 10.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Health Sciences › Medicine › Pharmacology (medical) › Flavonoids in Medical Research
- Keywords
- flavonoids, antioxidant activity, Caenorhabditis elegans, oxidative stress resistance, reactive oxygen species, superoxide dismutase, glutathione peroxidase, lipofuscin, protein carbonylation, sod-3, gst-4, hsp-16.2, antioxidant defense genes, Chinese herbal medicine
- MeSH terms
- Animals, Antioxidants, Dose-Response Relationship, Drug, Flavonoids, Plant Extracts, Caenorhabditis elegans, Reactive Oxygen Species, Toxicity Tests, Artemisia
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