The effects of autophagy on vascular endothelial cells induced by airborne PM2.5
Abstract
The purpose of this study was to examine the direct toxicity of PM2.5 collected from Beijing on human umbilical vein endothelial cells (HUVEC). A Cell Counting Kit 8 (CCK8) assay demonstrated that PM2.5 exposure decreased the proliferation of HUVECs in a dose-dependent manner. We also found that PM2.5 exposure induced autophagy in HUVECs, as evidenced by: (1) an increased number of double-membrane vesicles; (2) enhanced conversion and punctuation of the microtubule-associated protein light chain 3 (LC3); and (3) decreased levels of the selective autophagy substrate p62 in a time-dependent manner. Furthermore, promoting autophagy in PM2.5-exposed HUVECs with rapamycin increased the cell survival rate, whereas inhibiting autophagy via 3-methyladenine significantly decreased cell survival. These results demonstrate that PM2.5 exposure can induce cytotoxicity and autophagy in HUVECs and that autophagy play a protective role against PM2.5-induced cytotoxicity. The findings of the present study imply a direct toxic effect of PM2.5 on HUVECs and provide novel insight into the mechanism of cardiovascular diseases caused by PM2.5 exposure.
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PM2.5-induced oxidative stress could trigger autophagy in various cell types.
“It has been reported that PM2.5-induced oxidative stress could trigger autophagy in various cell types (Deng et al. 2013, Su et al. 2017; Zhou et al. 2017).”
From Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis (2018) · cites this paper as Zhou et al. 2017
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“5-induced oxidative stress could trigger autophagy in various cell types (Deng et al. 2013, Su et al. 2017; Zhou et al. 2017).”
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- Authors
- Zhou, Z., Shao, T., Qin, M., Miao, X., Chang, Y., Sheng, W., Wu, F., & Yu, Y.
- Affiliations
- Beijing University of Technology (CN)
- Chinese Research Academy of Environmental Sciences (CN)
- Ministry of Ecology and Environment (CN)
- Corresponding author
- Zhou, Zhixiang
- Yu, Yunjiang
- Venue
- Journal of Environmental Sciences
- Publisher
- Elsevier BV
- Published
- 2018-04
- Volume
- 66
- Pages
- 182-187
- ISSN
- 1001-0742 (ISSN-L)
- OpenAlex
- W2616406385
- Cited by
- 64
- References
- 23
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 9 · 2023: 5 · 2022: 12 · 2021: 7 · 2020: 13 · 2019: 10 · 2018: 3
- FWCI
- 3.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Environmental Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21677006, 21177119)
- Chinese Research Academy of Environmental Sciences (SKLECRA2016OFP)
- Chinese Academy of Sciences (KF2015-19)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Indoor Air Quality and Microbial Exposure
- Keywords
- PM2.5, autophagy, vascular endothelial cells, human umbilical vein endothelial cells, air pollution toxicity, endothelial cell proliferation, LC3 conversion, p62, rapamycin, 3-methyladenine, cardiovascular disease, Beijing air pollution
- MeSH terms
- Beijing, Air Pollutants, Autophagy, Cell Survival, Humans, Toxicity Tests, Particulate Matter, Human Umbilical Vein Endothelial Cells
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