The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Toxic effects of nanoplastics with different sizes and surface charges on epithelial-to-mesenchymal transition in A549 cells and the potential toxicological mechanism
Open accessAbstract
As a newly emerging hazardous material, airborne nanoplastics are easily inhaled and accumulated in human and animal alveoli. We previously found that polystyrene nanoplastics (PS-NPs) induced apoptosis and inflammation of human alveolar epithelial A549 cells, implying they increase the risk of pulmonary fibrosis. In this study, we investigated whether PS-NPs induce epithelial-to-mesenchymal transition (EMT), the prelude to lung fibrosis, in A549 cells. A549 cells treated with PS-NPs of different sizes and surface charges exhibited increased migration and EMT markers accompanied with up-regulation of reactive oxygen species (ROS) and NADPH oxidase 4 (NOX4), an ROS generator located in the mitochondria and endoplasmic reticulum (ER). Moreover, PS-NPs caused mitochondrial dysfunction as demonstrated by membrane potential changes and impaired cellular energy metabolism. PS-NPs also activated ER stress as indicated by the up-regulated ER stress markers. As expected, smaller PS-NPs with a positive surface charge had stronger effects. Furthermore, the effects of PS-NPs on A549 cells were reversed by NOX4 gene knock-down, which verified the involvement of NOX4. Our results suggest that PS-NPs induce EMT in A549 cells through multiple mechanisms, and NOX4 is a key mediator in this process. Our findings contribute to understanding the toxicological mechanisms of nanoplastics on the respiratory system.
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Cites
Mitochondrial Reactive Oxygen Species (ROS) and ROS-Induced ROS Release
Zorov, D. B., Juhaszova, M., & Sollott, S. J. (2014) · Physiological Reviews · doi:10.1152/physrev.00026.2013 · Cited by 5,383
NOX enzymes and the biology of reactive oxygen
Lambeth, J. D. (2004) · Nature Reviews Immunology · doi:10.1038/nri1312 · Cited by 3,125
Regulatory networks defining EMT during cancer initiation and progression
Craene, B. D., & Berx, G. (2013) · Nature Reviews Cancer · doi:10.1038/nrc3447 · Cited by 2,487
Pathways for degradation of plastic polymers floating in the marine environment
Gewert, B., Plassmann, M. M., & MacLeod, M. (2015) · Environmental Science: Processes & Impacts · doi:10.1039/c5em00207a · Cited by 2,168
Song, Y. K., Hong, S. H., Jang, M., Han, G. M., Jung, S. W., & Shim, W. J. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.6b06155 · Cited by 1,568
UPR, autophagy, and mitochondria crosstalk underlies the ER stress response
Senft, D., & Ronai, Z. A. (2015) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2015.01.002 · Cited by 1,121
Xu, M., Halimu, G., Zhang, Q., Song, Y., Fu, X., Li, Y., Li, Y., & Zhang, H. (2019) · Science of The Total Environment · doi:10.1016/j.scitotenv.2019.133794 · Cited by 659
Self-citation
Cited by
Microplastic sources, formation, toxicity and remediation: a review
Osman, A. I., Hosny, M., Eltaweil, A. S., Omar, S., Elgarahy, A. M., Farghali, M., Yap, P.-S., Wu, Y.-S., Nagandran, S., Batumalaie, K., et al. (2023) · Environmental Chemistry Letters · doi:10.1007/s10311-023-01593-3 · Cited by 816
Metabolomics Reveal Nanoplastic-Induced Mitochondrial Damage in Human Liver and Lung Cells
Lin, S., Zhang, H., Wang, C., Su, X.-L., Song, Y., Wu, P., Yang, Z., Wong, M.-H., Cai, Z., & Zheng, C. (2022) · Environmental Science & Technology · doi:10.1021/acs.est.2c03980 · Cited by 391
Self-citationPłuciennik, K., Sicińska, P., Misztal, W., & Bukowska, B. (2024) · Cells · doi:10.3390/cells13090768 · Cited by 132
Metadata
- Authors
- Halimu, G., Zhang, Q., Liu, L., Zhang, Z., Wang, X., Gu, W., Zhang, B., Dai, Y., Zhang, H., Zhang, C., & Xu, M.
- Venue
- Journal of Hazardous Materials
- Publisher
- Elsevier BV
- Published
- 2022-05
- Volume
- 430
- Pages
- 128485
- ISSN
- 0304-3894 (ISSN-L)
- OpenAlex
- W4212891862
- Cited by
- 221
- References
- 7
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Halimu, G., Zhang, Q., Liu, L., Zhang, Z., Wang, X., Gu, W., Zhang, B., Dai, Y., Zhang, H., Zhang, C., & Xu, M. (2022). Toxic effects of nanoplastics with different sizes and surface charges on epithelial-to-mesenchymal transition in A549 cells and the potential toxicological mechanism. Journal of Hazardous Materials. https://doi.org/10.1016/j.jhazmat.2022.128485
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