Cited by 10Enzyme Structure and FunctionBiochemical and biochemical processesEnzyme Production and Characterization

Structural and Functional Characterization of a Gene Cluster Responsible for Deglycosylation of C-glucosyl Flavonoids and Xanthonoids by Deinococcus aerius

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Furlanetto, Valentina, Kalyani, Dayanand C., Kostelac, Anja, Puc, Jolanta, Haltrich, Dietmar, Hällberg, B. Martin, Divne, Christina·2024·Journal of Molecular Biology

Abstract

Plant C-glycosylated aromatic polyketides are important for plant and animal health. These are specialized metabolites that perform functions both within the plant, and in interaction with soil or intestinal microbes. Despite the importance of these plant compounds, there is still limited knowledge of how they are metabolized. The Gram-positive aerobic soil bacterium Deinococcus aerius strain TR0125 and other Deinococcus species thrive in a wide range of harsh environments. In this work, we identified a C-glycoside deglycosylation gene cluster in the genome of D. aerius. The cluster includes three genes coding for a GMC-type oxidoreductase (DaCGO1) that oxidizes the glucosyl C3 position in aromatic C-glucosyl compounds, which in turn provides the substrate for the C-glycoside deglycosidase (DaCGD; composed of α+β subunits) that cleaves the glucosyl-aglycone C-C bond. Our results from size-exclusion chromatography, single particle cryo-electron microscopy and X-ray crystallography show that DaCGD is an α 2 β 2 heterotetramer, which represents a novel oligomeric state among bacterial CGDs. Importantly, the high-resolution X-ray structure of DaCGD provides valuable insights into the activation of the catalytic hydroxide ion by Lys261. DaCGO1 is specific for the 6-C-glucosyl flavones isovitexin, isoorientin and the 2-C-glucosyl xanthonoid mangiferin, and the subsequent C-C-bond cleavage by DaCGD generated apigenin, luteolin and norathyriol, respectively. Of the substrates tested,…

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Crossref · 10.1016/j.jmb.2024.168547

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  • The gut microbiota serves a critical role in the deglycosylation and subsequent metabolism of MGF, representing a key determinant of its systemic availability and pharmacological effects.

    “The gut microbiota serves a critical role in the deglycosylation and subsequent metabolism of MGF, representing a key determinant of its systemic availability and pharmacological effects (24).”

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Authors
Furlanetto, V., Kalyani, D. C., Kostelac, A., Puc, J., Haltrich, D., Hällberg, B. M., & Divne, C.
Affiliations
  • KTH Royal Institute of Technology (SE)
  • BOKU University (AT)
  • Karolinska Institutet (SE)
Corresponding author
Divne, Christina
Venue
Journal of Molecular Biology
Publisher
Elsevier BV
Published
2024-05
Volume
436
Issue
9
Pages
168547
ISSN
0022-2836 (ISSN-L)
OpenAlex
W4392907664
Cited by
9
References
61
Citations by year
2026: 3 · 2025: 2 · 2024: 4
FWCI
0.88
Type
journal article
Language
en
Open access
Locations
  • Journal of Molecular Biology (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
KTH Royal Institute of Technology
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Keywords
enzyme structure, isovitexin, isoorientin, mangiferin, apigenin, luteolin, norathyriol, soil bacteria
MeSH terms
Bacterial Proteins, Flavonoids, Genes, Bacterial, Multigene Family, Glycosides, Glycosylation, Models, Molecular, Crystallography, X-Ray, Deinococcus, Xanthones

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