Oligodendrocyte precursor cells express a functional chemokine receptor CCR3: Implications for myelination
Abstract
Myelination in the central nervous system requires an accurate interplay between oligodendrocyte precursor cells (OPC) and axons. By as yet not fully understood mechanisms, OPC proliferate, migrate to the axon to be myelinated and finally differentiate into mature oligodendrocytes. The recent finding that OPC express CXC chemokine receptors led us to the investigation of the expression and functional importance of CC chemokine receptors. Using RT-PCR, we show that primary OPC from neonatal rats express CCR3, while CCR1, CCR2, CCR4, CCR5, and CCR7 are not expressed. Immunofluorescence staining of OPC could further demonstrate protein expression of CCR3. A rise of intracellular Ca2+ upon stimulation with the appropriate ligand CCL11 showed that this receptor is functional. Moreover, CCL11 led to a concentration specific increase in proliferation, inhibition of migration, and augmentation of differentiation in primary OPC. Thus, CCR3 may influence the process of myelination. This is of general importance for both developmental tissue patterning and for repair processes in demyelinating diseases like multiple sclerosis.
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OPCs have been suggested to express the primary CCL11 receptor CCR3, and elevated CCL11 levels are associated with MS disease duration and severity.
“Moreover, OPCs have been suggested to express the primary CCL11 receptor, CCR360 and elevated CCL11 levels are associated with MS disease duration and severity61,62.”
From Senescent-like microglia limit remyelination through the senescence associated secretory phenotype (2025) · cites this paper as [60]
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- Authors
- Maysami, S., Nguyen, D., Zobel, F., Heine, S., Höpfner, M., & Stangel, M.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Stiftung Charité (DE)
- Venue
- Journal of Neuroimmunology
- Publisher
- Elsevier BV
- Published
- 2006-09
- Volume
- 178
- Issue
- 1-2
- Pages
- 17-23
- ISSN
- 0165-5728 (ISSN-L)
- OpenAlex
- W2134505623
- Cited by
- 25
- References
- 30
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 1
- FWCI
- 0.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroimmunology (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Keywords
- oligodendrocyte precursor cells, CCR3, CC chemokine receptors, myelination, eotaxin, oligodendrocyte differentiation, cell proliferation, cell migration, intracellular calcium signaling, demyelinating disease, multiple sclerosis, central nervous system development
- MeSH terms
- Animals, Cell Differentiation, Cell Movement, Cells, Cultured, Immunohistochemistry, Myelin Sheath, Oligodendroglia, RNA, Messenger, Stem Cells, Rats, Sprague-Dawley, Receptors, Chemokine, Chemokines, CC, Reverse Transcriptase Polymerase Chain Reaction, Cell Proliferation, Rats, Receptors, CCR3, Chemokine CCL11
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