Consumption of ellagic acid and dihydromyricetin synergistically protects against UV-B induced photoaging, possibly by activating both TGF-β1 and wnt signaling pathways
Abstract
Ellagic acid (EGA) and dihydromyricetin (DHM) are both found in fruits and vegetables are used for anti-aging treatment for the skin. The anti-photoaging efficacy of EGA and DHM was investigated in UV-B irradiated skin in vivo and the involvement of transforming growth factor (TGF)-β1 and wnt signaling pathways were examined in vitro. HaCaT cells were treated with either 50μM EGA, 50μM DHM or 25μM EGA+25μM DHM before 100mJ/cm 2 UV-B exposure, and then oxidative stress and inflammation was measured. The involvement of TGF-β1 and wnt signaling was measured using their inhibitors, respectively, in HaCaT cells. Mice were fed a high fat diet with either 0.7% cellulose, 0.7% EGA, 0.7% DHM or 0.35% EGA+0.35% DHM for 3weeks and the dorsal skin of the mice had UV-B irradiation. 3% cellulose, 3% EGA, 3% DHM or 1.5% EGA+1.5% DHM in 1,3-buthylene glycol was applied onto the dorsal skin at 30min before 1 MED UV-B exposure. In 100mJ/cm 2 UVB irradiation, EGA and DHM mainly decreased oxidative stress and inflammation, respectively in HaCaT cells. Their activities were blocked by the TGF-β1 inhibitor, indicating their actions were mediated by TGF-β1 signaling (TGF-β1➔pSmad3➔Smad7). DHM enhanced wnt signaling by increasing β-catenin and decreasing Dickkopf-related protein-1. In mice, 1 MED UV-B exposure induced sunburn, redness, and blistering. EGA, DHM and especially EGA+DHM lessened their severity. UV-B increased epidermal thickness and damaged epidermal nucleus and cell structures. DHM and especially EGA+DHM prevented damage to the nucleus and cell structures. Expressions of circulating and dorsal skin IL-1β and TNF-α mRNA were lower in descending order of: control, EGA, DHM, EGA+DHM and normal-control. In conclusion, the consumption of EGA+DHM had a synergistically protective action against UV-B damage in the skin tissues of mice and HaCaT cells, and it may be associated with activating of both TGF-β1 and wnt signaling.
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Beneficial effects of dihydromyricetin and ellagic acid may be associated with activation of both TGF-beta1 and wnt signaling.
“Their beneficial effects may be associated with the activation of both TGF-β1 and wnt signaling [106].”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [106]
Oral supplementation with a combination of dihydromyricetin and ellagic acid had a synergistically protective action against UVB damage in dorsal skin tissue of mice, manifested as ameliorated erythema and reduced expression of pro-inflammatory cytokines and MMP-1, compared with separate treatment.
“Oral supplementation with a combination of dihydromyricetin and ellagic acid had a synergistically protective action against UVB damage in the dorsal skin tissue of mice, manifested as significantly ameliorated erythema, and markedly reduced the expression of pro-inflammatory cytokines and MMP-1, compared with the separate treatment of dihydromyricetin and ellagic acid.”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [106]
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Boosting the Photoaged Skin: The Potential Role of Dietary Components
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Self-citation
- Authors
- Moon, N. R., Kang, S., & Park, S.
- Affiliations
- Hoseo University (KR)
- Corresponding author
- Park, Sunmin
- Venue
- Journal of Photochemistry and Photobiology B Biology
- Publisher
- Elsevier BV
- Published
- 2018-01
- Volume
- 178
- Pages
- 92-100
- ISSN
- 1011-1344 (ISSN-L)
- OpenAlex
- W2767220576
- Cited by
- 47
- References
- 35
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 9 · 2023: 5 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 7
- FWCI
- 3.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Photochemistry and Photobiology B Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Korean National Research Foundation (NRF-2015R1D1A3A01019577)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- ellagic acid, dihydromyricetin, photoaging, UVB irradiation, TGF-β1 signaling, Wnt signaling, Smad3, Smad7, β-catenin, Dickkopf-related protein 1, oxidative stress, skin inflammation, IL-1β, TNF-α, HaCaT keratinocytes, topical application, synergistic protection
- MeSH terms
- Animals, Cell Line, Cell Survival, Dose-Response Relationship, Drug, Drug Synergism, Ellagic Acid, Gene Expression Regulation, Humans, Male, Mice, Inbred ICR, Radiation-Protective Agents, RNA, Messenger, Skin Aging, Oxidative Stress, Matrix Metalloproteinase 1, Flavonols, Mice, Interleukin-1beta, Transforming Growth Factor beta1, Wnt Signaling Pathway
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