Regulation of biohydrogen production by protonophores in novel green microalgae Parachlorella kessleri
Abstract
The green microalgae Parachlorella kessleri RA-002 isolated in Armenia can produce biohydrogen (H 2) during oxygenic photosynthesis. Addition of protonophores, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (DNF) enhances H 2 yield in P. kessleri. The maximal H 2 yield of ~2.20 and 2.08 mmol L-1 was obtained in the presence of 15 μM CCCP and 50 μM DNF, respectively. During dark conditions H 2 production by P. kessleri was not observed even in the presence of protonophores, indicating that H 2 formation in these algae was mediated by light conditions. The enhancing effect of protonophores can be coupled with dissipation of proton motive force across thylakoid membrane in P. kessleri, facilitating the availability of protons and electrons to [Fe-Fe]-hydrogenase, which led to formation of H 2. At the same time H 2 production was not observed in the presence of diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a specific inhibitor of PS II. Moreover, diuron inhibits H 2 yield in P. kessleri in the presence of protonophores. The inhibitory effect of diuron coupled with suppression of electron transfer from PS II. The results showed that in these algae operates PS II-dependent pathway of H 2 generation. This study is important for understanding of the mechanisms of H 2 production by green microalgae P. kessleri and developing of its biotechnology.
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The wide range of variation in the chemical composition of algae gives potential to generate a diversity of fuel types such as bioethanol, biohydrogen, biomethane, bio-oil, and biodiesel.
“Given the wide range of variation in the chemical composition of algae, the potential exits to generate a diversity of fuel types from them such as bioethanol [3], biohydrogen [4], biomethane [5], bio-oil [6], and biodiesel [7].”
From Key Targets for Improving Algal Biofuel Production (2021) · cites this paper as [4]
Under conditions of low O2, the electrons are channelled to an [Fe-Fe] hydrogenase enzyme, which utilises H+ and the electron source to generate H2.
“However, under conditions of low O2, the electrons are channelled to an [Fe-Fe] hydrogenase enzyme, which utilises H + and the electron source to generate H2 [4].”
From Key Targets for Improving Algal Biofuel Production (2021) · cites this paper as [4]
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Key Targets for Improving Algal Biofuel Production
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- Authors
- Manoyan, J., Gabrielyan, L., Kozel, N., & Trchounian, A.
- Affiliations
- Yerevan State University (AM)
- National Academy of Sciences of Belarus (BY)
- Venue
- Journal of Photochemistry and Photobiology B Biology
- Publisher
- Elsevier BV
- Published
- 2019-10
- Volume
- 199
- Pages
- 111597
- ISSN
- 1011-1344 (ISSN-L)
- OpenAlex
- W2969915386
- Cited by
- 37
- References
- 28
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 3 · 2023: 7 · 2022: 7 · 2021: 10 · 2020: 4
- FWCI
- 1.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Photochemistry and Photobiology B Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Research Grants from the Committee of Science
- Ministry of Education and Science of Armenia (18T-1F045)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- biohydrogen production, Parachlorella kessleri, protonophores, CCCP, 2,4-dinitrophenol, diuron, photosystem II, [FeFe]-hydrogenase, thylakoid membranes, oxygenic photosynthesis, green microalgae, electron transfer
- MeSH terms
- Chlorophyta, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Diuron, Electron Transport, Hydrogen, Hydrogenase, Iron-Sulfur Proteins, Light, Oxidation-Reduction, Oxygen, Photosynthesis, Protons, Signal Transduction, Photosensitizing Agents, 2,4-Dinitrophenol, Microalgae
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