Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651
Abstract
The proteome of Aggregatibacter actinomycetemcomitans HK1651 (JP2 clone) and immunoreactive antigens were studied by two-dimensional (2D) gel electrophoresis, matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS), and 2D immunoblotting. The highly leukotoxic JP2 clone of A. actinomycetemcomitans is strongly associated with aggressive periodontitis (AgP) in adolescents of North-West African descent and the pathogenicity of this bacterium is of major interest. Hence, we developed a comprehensive 2D proteome reference map of A. actinomycetemcomitans proteins with 167 identified spots representing 114 different proteins of which 15 were outer membrane proteins. To unravel immunoreactive antigens, we applied 2D-gel and subsequent immunoblotting analyses using sera from five individuals with A. actinomycetemcomitans infections and one healthy control. The analysis revealed 32 immunoreactive proteins. Antibodies to two outer membrane proteins, YaeT (85 kDa) and Omp39 (39 kDa), not previously described as immunoreactive, were found only in subjects with current or previous A. actinomycetemcomitans JP2 infection. Further proteome-based studies of A. actinomycetemcomitans combined with analyses of the humoral immune response and targeted against outer membrane proteins may provide important insight into the host relationship of this important pathogen.
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Proteomic applications have been applied to understand changes in the proteome that diverge states of periodontal health from disease and to further characterize various periodontal disease states, including gingivitis, mild, moderate, chronic, and aggressive periodontitis.
“Proteomic applications have been applied to understand changes in the proteome that diverge states of periodontal health from disease and to further characterize various periodontal disease states, including gingivitis, mild, moderate, chronic, and aggressive periodontitis.70, 101, 102, 103, 104”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [103]
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BackgroundThe cell envelope proteome of Aggregatibacter actinomycetemcomitans
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ResultProteomic Investigations of In Vitro and In Vivo Models of Periodontal Disease
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“Two-dimensional electrophoresis, MALDI-TOF mass spectrometry, and 2D immunoblotting have also been employed ([23]).”
MethodAggregatibacter actinomycetemcomitans
Fu, Y. (2023) · doi:10.33612/diss.621543190
- Authors
- Rylev, M., Abduljabar, A. B., Reinholdt, J., Ennibi, O.-K., Haubek, D., Birkelund, S., & Kilian, M.
- Affiliations
- Aarhus University (DK)
- Mohammed V University (MA)
- Corresponding author
- Rylev, Mette
- Venue
- Journal of Proteomics
- Publisher
- Elsevier BV
- Published
- 2011-11
- Volume
- 74
- Issue
- 12
- Pages
- 2972-2985
- ISSN
- 1874-3919 (ISSN-L)
- OpenAlex
- W2000272161
- Cited by
- 20
- References
- 47
- Citations by year
- 2023: 1 · 2022: 3 · 2021: 3 · 2019: 4 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 4 · 2012: 1
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteomics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Danish Dental Society's Research Foundation
- Karen Elise Jensen Foundation
- Faculty of Health Sciences, Aarhus University
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- Aggregatibacter actinomycetemcomitans, proteome, outer membrane proteins, aggressive periodontitis, humoral immune response, host-pathogen interaction, bacterial virulence
- MeSH terms
- Antigens, Bacterial, Bacterial Outer Membrane Proteins, Humans, Immunosuppressive Agents, Periodontitis, Immunoblotting, Electrophoresis, Gel, Two-Dimensional, Pasteurellaceae, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteomics
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