Development of an MRM assay panel with application to biobank samples from patients with myocardial infarction
Abstract
As part of a Swedish national cardiological research initiative, the development of a quantitative MRM assay is reported for the quantification of eleven putative cardiovascular disease markers. Within the study, patient samples from the LUNDHEARTGENE biobank were processed and nanoLC-MS/MS analysis was performed together with a stable isotope dilution strategy for absolute quantification of the target proteins. Excellent linear regressions were achieved for 9 of the 11 peptides with LOQ ranged in the attomolar range. We have utilized the assay for the screening of plasma samples from patients with chest pain, and performed a comparative analysis of patients with ST-segment elevation myocardial infarction and chest pain due to other causes. The assay demonstrates high reproducibility and correlate with clinical findings. Strong correlations were found for several of the apolipoproteins and their respective lipid subfractions (LDL, HDL or triglycerides). APOC1, APOC2 and APOE were elevated in patients with STEMI. Biological significance An MRM assay were developed for putative cardiovascular disease markers as target proteins, and applied to biobanking sample material. The comparative analysis of patients with ST-segment elevation myocardial infarction and chest pain due to other causes showed elevated levels of APOC1, APOC2 and APOE in patients with STEMI. These observations raise interesting novel hypotheses about the role of apolipoproteins C1, C2 and E in the pathophysiolo…
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Various LC-MS/MS assays for multiplexed quantification of apolipoproteins or phenotyping of apoE have been reported.
“Various LC-MS/MS assays for multiplexed quantification of apolipoproteins (14-23) or phenotyping of apoE (24-27) have been reported.”
From Automated multiplex LC-MS/MS assay for quantifying serum apolipoproteins A-I, B, C-I, C-II, C-III, and E with qualitative apolipoprotein E phenotyping (2016) · cites this paper as [20]
Cites
Vaisar, T., Pennathur, S., Green, P. S., Gharib, S. A., Hoofnagle, A. N., Cheung, M. C., Byun, J., Vuletic, S., Kassim, S., Singh, P., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci26206 · Cited by 942 · see citing works
On the Development of Plasma Protein Biomarkers
Surinova, S., Schiess, R., Hüttenhain, R., Cerciello, F., Wollscheid, B., & Aebersold, R. (2011) · Journal of Proteome Research · doi:10.1021/pr1008515 · Cited by 334 · cited 2× · see citing works
“[25] Surinova S, Schiess R, Huttenhain R, Cerciello F, Wollscheid B, Aebersold R.”
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van den Broek, I., Romijn, F. P. H. T. M., Nouta, J., van der Laarse, A., Drijfhout, J. W., Smit, N. P. M., van der Burgt, Y. E. M., & Cobbaert, C. M. (2016) · Clinical Chemistry · doi:10.1373/clinchem.2015.246702 · Cited by 105 · see citing works
Kahl, K. G., Stapel, B., & Frieling, H. (2019) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2018.09.004 · Cited by 47 · see citing works
- Authors
- Rezeli, M., Végvári, Á., Donnarumma, F., Gidlöf, O., Smith, J. G., Erlinge, D., & Marko-Varga, G.
- Affiliations
- Lund University (SE)
- Corresponding author
- Rezeli, Melinda
- Venue
- Journal of Proteomics
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 87
- Pages
- 16-25
- ISSN
- 1874-3919 (ISSN-L)
- OpenAlex
- W1990839024
- Cited by
- 33
- References
- 2
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 9 · 2013: 1
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteomics (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Swedish Foundation for Strategic Research, “TOTAL-AMI”, Swedish Academy of Pharmaceutical Sciences, Swedish Research Council, Vinnova, Ingabritt & Arne Lundbergs forskningsstiftelse
- Crafoord Foundation
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- cardiovascular biomarkers, ST-elevation myocardial infarction, APOC1, apolipoprotein C2, apolipoprotein E, apolipoproteins, chest pain, biobank samples, absolute quantification
- MeSH terms
- Apolipoproteins, Female, Humans, Male, Myocardial Infarction, Mass Spectrometry, Reproducibility of Results, Biological Specimen Banks
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